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Green extraction, chemical composition, and in vitro antioxidant activity of theabrownins from Kangzhuan dark tea
Theabrownins (TBs) in dark tea have received increasing attention for their multiple health benefits. In this study, ultrasound assisted extraction with deep eutectic solvent (UAE-DES) was developed for the extraction of TBs from Kangzhuan dark tea (KZDT). The highest yield (12.59%) of TBs was obtai...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9589173/ https://www.ncbi.nlm.nih.gov/pubmed/36300163 http://dx.doi.org/10.1016/j.crfs.2022.10.019 |
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author | Liu, Yi Liu, Hong-Yan Yang, Xiao Zhu, Fan Wu, Ding-Tao Li, Hua-Bin Gan, Ren-You |
author_facet | Liu, Yi Liu, Hong-Yan Yang, Xiao Zhu, Fan Wu, Ding-Tao Li, Hua-Bin Gan, Ren-You |
author_sort | Liu, Yi |
collection | PubMed |
description | Theabrownins (TBs) in dark tea have received increasing attention for their multiple health benefits. In this study, ultrasound assisted extraction with deep eutectic solvent (UAE-DES) was developed for the extraction of TBs from Kangzhuan dark tea (KZDT). The highest yield (12.59%) of TBs was obtained using UAE-choline (ChCl)/malic acid (MA) with a liquid to solid ratio of 20:1 (v/w), ultrasonic power of 577 W, ultrasonic time of 25 min, and water content of 30%. TBs were further eluded by silica gel to obtain six theabrownine fractions (TBFs), namely, TBFs1, TBFs2, TBFs3, TBFs4, TBFs5, and TBFs6. LC-MS/MS revealed that flavonoids, terpenes, phenolic acids, alkaloids, lipids, and amino acids are the leading components of TBFs. The TBFs4, with the DPPH, ABTS, and FRAP values of 45.08 ± 0.42 μM Ascorbic acid/g DW, 178.52 ± 0.29 μM Trolox/g DW, and 370.85 ± 6.00 μM Fe(II)/g DW, respectively, showed the highest antioxidant activity among all the TBFs. Overall, this study first provided the evidence that UAE-ChCl/MA combining with silica gel was effective to extract TBs from KZDT, and the 6,7-dihydroxycoumarin-6-glucoside and neohesperidin were found as the leading compounds in the TBFs, providing a guidance for the chemical research and further utilization of dark tea and its TBs. |
format | Online Article Text |
id | pubmed-9589173 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-95891732022-10-25 Green extraction, chemical composition, and in vitro antioxidant activity of theabrownins from Kangzhuan dark tea Liu, Yi Liu, Hong-Yan Yang, Xiao Zhu, Fan Wu, Ding-Tao Li, Hua-Bin Gan, Ren-You Curr Res Food Sci Articles from the special issue: 6th International Symposium on Phytochemicals in Medicine and Food, edited by Jianbo Xiao, Jinping Si and Huifan Liu Theabrownins (TBs) in dark tea have received increasing attention for their multiple health benefits. In this study, ultrasound assisted extraction with deep eutectic solvent (UAE-DES) was developed for the extraction of TBs from Kangzhuan dark tea (KZDT). The highest yield (12.59%) of TBs was obtained using UAE-choline (ChCl)/malic acid (MA) with a liquid to solid ratio of 20:1 (v/w), ultrasonic power of 577 W, ultrasonic time of 25 min, and water content of 30%. TBs were further eluded by silica gel to obtain six theabrownine fractions (TBFs), namely, TBFs1, TBFs2, TBFs3, TBFs4, TBFs5, and TBFs6. LC-MS/MS revealed that flavonoids, terpenes, phenolic acids, alkaloids, lipids, and amino acids are the leading components of TBFs. The TBFs4, with the DPPH, ABTS, and FRAP values of 45.08 ± 0.42 μM Ascorbic acid/g DW, 178.52 ± 0.29 μM Trolox/g DW, and 370.85 ± 6.00 μM Fe(II)/g DW, respectively, showed the highest antioxidant activity among all the TBFs. Overall, this study first provided the evidence that UAE-ChCl/MA combining with silica gel was effective to extract TBs from KZDT, and the 6,7-dihydroxycoumarin-6-glucoside and neohesperidin were found as the leading compounds in the TBFs, providing a guidance for the chemical research and further utilization of dark tea and its TBs. Elsevier 2022-10-17 /pmc/articles/PMC9589173/ /pubmed/36300163 http://dx.doi.org/10.1016/j.crfs.2022.10.019 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Articles from the special issue: 6th International Symposium on Phytochemicals in Medicine and Food, edited by Jianbo Xiao, Jinping Si and Huifan Liu Liu, Yi Liu, Hong-Yan Yang, Xiao Zhu, Fan Wu, Ding-Tao Li, Hua-Bin Gan, Ren-You Green extraction, chemical composition, and in vitro antioxidant activity of theabrownins from Kangzhuan dark tea |
title | Green extraction, chemical composition, and in vitro antioxidant activity of theabrownins from Kangzhuan dark tea |
title_full | Green extraction, chemical composition, and in vitro antioxidant activity of theabrownins from Kangzhuan dark tea |
title_fullStr | Green extraction, chemical composition, and in vitro antioxidant activity of theabrownins from Kangzhuan dark tea |
title_full_unstemmed | Green extraction, chemical composition, and in vitro antioxidant activity of theabrownins from Kangzhuan dark tea |
title_short | Green extraction, chemical composition, and in vitro antioxidant activity of theabrownins from Kangzhuan dark tea |
title_sort | green extraction, chemical composition, and in vitro antioxidant activity of theabrownins from kangzhuan dark tea |
topic | Articles from the special issue: 6th International Symposium on Phytochemicals in Medicine and Food, edited by Jianbo Xiao, Jinping Si and Huifan Liu |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9589173/ https://www.ncbi.nlm.nih.gov/pubmed/36300163 http://dx.doi.org/10.1016/j.crfs.2022.10.019 |
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