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Caveolin-1, GATA-3, and Ki67 expressions and their correlation with pathological findings in canine bladder urothelial carcinoma
The bladder urothelial carcinoma (UC) represents ~2% of malignant neoplasms in dogs and is a therapeutic challenge in veterinary medicine. Although it is considered the most common bladder cancer in dogs, few previous studies have investigated different markers that correlate with clinical and patho...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9589490/ https://www.ncbi.nlm.nih.gov/pubmed/36299636 http://dx.doi.org/10.3389/fvets.2022.986269 |
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author | Govoni, Verônica Mollica Pigoli, Claudio Brambilla, Eleonora Ruiz Sueiro, Felipe Augusto Torres Neto, Rafael Laufer-Amorim, Renee Gomes Quitzan, Juliany Grieco, Valeria Fonseca-Alves, Carlos Eduardo |
author_facet | Govoni, Verônica Mollica Pigoli, Claudio Brambilla, Eleonora Ruiz Sueiro, Felipe Augusto Torres Neto, Rafael Laufer-Amorim, Renee Gomes Quitzan, Juliany Grieco, Valeria Fonseca-Alves, Carlos Eduardo |
author_sort | Govoni, Verônica Mollica |
collection | PubMed |
description | The bladder urothelial carcinoma (UC) represents ~2% of malignant neoplasms in dogs and is a therapeutic challenge in veterinary medicine. Although it is considered the most common bladder cancer in dogs, few previous studies have investigated different markers that correlate with clinical and pathological parameters. Therefore, this study aimed to evaluate Caveolin-1, GATA-3, and Ki67 immunostaining in canine UC samples to evaluate their correlations with histopathological variables. Thirty tumor samples were obtained, and Caveolin-1, GATA-3, and Ki67 expression was assessed by immunohistochemistry and associated with pathological factors by univariate and multivariate analyses. Among the histopathological findings, lymphatic invasion was identified in 53.33% of the tumors, and the mean mitotic count (MC) was 31.82 ± 26.26. Caveolin-1 showed mild-to-high cytoplasmic expression in neoplastic cells, whereas GATA-3 showed mild-to-high nuclear expression. The Ki67 expression revealed a mean of 24.14 ± 16.88% positive cells. In the univariate analysis, no association was found between each marker and the pathological findings. On the other hand, in multivariate analysis, we identified a positive correlation between GATA-3 and MC and a negative correlation between Caveolin-1 and MC. Moreover, lymphatic invasion was positively correlated with histological type and grade, and negatively correlated with MC. In addition, the histological type was positively correlated with the histological grade. Overall, our results indicate that Caveolin-1 and GATA-3 expression could be promising markers for bladder UC aggressiveness. |
format | Online Article Text |
id | pubmed-9589490 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-95894902022-10-25 Caveolin-1, GATA-3, and Ki67 expressions and their correlation with pathological findings in canine bladder urothelial carcinoma Govoni, Verônica Mollica Pigoli, Claudio Brambilla, Eleonora Ruiz Sueiro, Felipe Augusto Torres Neto, Rafael Laufer-Amorim, Renee Gomes Quitzan, Juliany Grieco, Valeria Fonseca-Alves, Carlos Eduardo Front Vet Sci Veterinary Science The bladder urothelial carcinoma (UC) represents ~2% of malignant neoplasms in dogs and is a therapeutic challenge in veterinary medicine. Although it is considered the most common bladder cancer in dogs, few previous studies have investigated different markers that correlate with clinical and pathological parameters. Therefore, this study aimed to evaluate Caveolin-1, GATA-3, and Ki67 immunostaining in canine UC samples to evaluate their correlations with histopathological variables. Thirty tumor samples were obtained, and Caveolin-1, GATA-3, and Ki67 expression was assessed by immunohistochemistry and associated with pathological factors by univariate and multivariate analyses. Among the histopathological findings, lymphatic invasion was identified in 53.33% of the tumors, and the mean mitotic count (MC) was 31.82 ± 26.26. Caveolin-1 showed mild-to-high cytoplasmic expression in neoplastic cells, whereas GATA-3 showed mild-to-high nuclear expression. The Ki67 expression revealed a mean of 24.14 ± 16.88% positive cells. In the univariate analysis, no association was found between each marker and the pathological findings. On the other hand, in multivariate analysis, we identified a positive correlation between GATA-3 and MC and a negative correlation between Caveolin-1 and MC. Moreover, lymphatic invasion was positively correlated with histological type and grade, and negatively correlated with MC. In addition, the histological type was positively correlated with the histological grade. Overall, our results indicate that Caveolin-1 and GATA-3 expression could be promising markers for bladder UC aggressiveness. Frontiers Media S.A. 2022-10-10 /pmc/articles/PMC9589490/ /pubmed/36299636 http://dx.doi.org/10.3389/fvets.2022.986269 Text en Copyright © 2022 Govoni, Pigoli, Brambilla, Ruiz Sueiro, Torres Neto, Laufer-Amorim, Gomes Quitzan, Grieco and Fonseca-Alves. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Veterinary Science Govoni, Verônica Mollica Pigoli, Claudio Brambilla, Eleonora Ruiz Sueiro, Felipe Augusto Torres Neto, Rafael Laufer-Amorim, Renee Gomes Quitzan, Juliany Grieco, Valeria Fonseca-Alves, Carlos Eduardo Caveolin-1, GATA-3, and Ki67 expressions and their correlation with pathological findings in canine bladder urothelial carcinoma |
title | Caveolin-1, GATA-3, and Ki67 expressions and their correlation with pathological findings in canine bladder urothelial carcinoma |
title_full | Caveolin-1, GATA-3, and Ki67 expressions and their correlation with pathological findings in canine bladder urothelial carcinoma |
title_fullStr | Caveolin-1, GATA-3, and Ki67 expressions and their correlation with pathological findings in canine bladder urothelial carcinoma |
title_full_unstemmed | Caveolin-1, GATA-3, and Ki67 expressions and their correlation with pathological findings in canine bladder urothelial carcinoma |
title_short | Caveolin-1, GATA-3, and Ki67 expressions and their correlation with pathological findings in canine bladder urothelial carcinoma |
title_sort | caveolin-1, gata-3, and ki67 expressions and their correlation with pathological findings in canine bladder urothelial carcinoma |
topic | Veterinary Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9589490/ https://www.ncbi.nlm.nih.gov/pubmed/36299636 http://dx.doi.org/10.3389/fvets.2022.986269 |
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