Cargando…

Caveolin-1, GATA-3, and Ki67 expressions and their correlation with pathological findings in canine bladder urothelial carcinoma

The bladder urothelial carcinoma (UC) represents ~2% of malignant neoplasms in dogs and is a therapeutic challenge in veterinary medicine. Although it is considered the most common bladder cancer in dogs, few previous studies have investigated different markers that correlate with clinical and patho...

Descripción completa

Detalles Bibliográficos
Autores principales: Govoni, Verônica Mollica, Pigoli, Claudio, Brambilla, Eleonora, Ruiz Sueiro, Felipe Augusto, Torres Neto, Rafael, Laufer-Amorim, Renee, Gomes Quitzan, Juliany, Grieco, Valeria, Fonseca-Alves, Carlos Eduardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9589490/
https://www.ncbi.nlm.nih.gov/pubmed/36299636
http://dx.doi.org/10.3389/fvets.2022.986269
_version_ 1784814317635371008
author Govoni, Verônica Mollica
Pigoli, Claudio
Brambilla, Eleonora
Ruiz Sueiro, Felipe Augusto
Torres Neto, Rafael
Laufer-Amorim, Renee
Gomes Quitzan, Juliany
Grieco, Valeria
Fonseca-Alves, Carlos Eduardo
author_facet Govoni, Verônica Mollica
Pigoli, Claudio
Brambilla, Eleonora
Ruiz Sueiro, Felipe Augusto
Torres Neto, Rafael
Laufer-Amorim, Renee
Gomes Quitzan, Juliany
Grieco, Valeria
Fonseca-Alves, Carlos Eduardo
author_sort Govoni, Verônica Mollica
collection PubMed
description The bladder urothelial carcinoma (UC) represents ~2% of malignant neoplasms in dogs and is a therapeutic challenge in veterinary medicine. Although it is considered the most common bladder cancer in dogs, few previous studies have investigated different markers that correlate with clinical and pathological parameters. Therefore, this study aimed to evaluate Caveolin-1, GATA-3, and Ki67 immunostaining in canine UC samples to evaluate their correlations with histopathological variables. Thirty tumor samples were obtained, and Caveolin-1, GATA-3, and Ki67 expression was assessed by immunohistochemistry and associated with pathological factors by univariate and multivariate analyses. Among the histopathological findings, lymphatic invasion was identified in 53.33% of the tumors, and the mean mitotic count (MC) was 31.82 ± 26.26. Caveolin-1 showed mild-to-high cytoplasmic expression in neoplastic cells, whereas GATA-3 showed mild-to-high nuclear expression. The Ki67 expression revealed a mean of 24.14 ± 16.88% positive cells. In the univariate analysis, no association was found between each marker and the pathological findings. On the other hand, in multivariate analysis, we identified a positive correlation between GATA-3 and MC and a negative correlation between Caveolin-1 and MC. Moreover, lymphatic invasion was positively correlated with histological type and grade, and negatively correlated with MC. In addition, the histological type was positively correlated with the histological grade. Overall, our results indicate that Caveolin-1 and GATA-3 expression could be promising markers for bladder UC aggressiveness.
format Online
Article
Text
id pubmed-9589490
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-95894902022-10-25 Caveolin-1, GATA-3, and Ki67 expressions and their correlation with pathological findings in canine bladder urothelial carcinoma Govoni, Verônica Mollica Pigoli, Claudio Brambilla, Eleonora Ruiz Sueiro, Felipe Augusto Torres Neto, Rafael Laufer-Amorim, Renee Gomes Quitzan, Juliany Grieco, Valeria Fonseca-Alves, Carlos Eduardo Front Vet Sci Veterinary Science The bladder urothelial carcinoma (UC) represents ~2% of malignant neoplasms in dogs and is a therapeutic challenge in veterinary medicine. Although it is considered the most common bladder cancer in dogs, few previous studies have investigated different markers that correlate with clinical and pathological parameters. Therefore, this study aimed to evaluate Caveolin-1, GATA-3, and Ki67 immunostaining in canine UC samples to evaluate their correlations with histopathological variables. Thirty tumor samples were obtained, and Caveolin-1, GATA-3, and Ki67 expression was assessed by immunohistochemistry and associated with pathological factors by univariate and multivariate analyses. Among the histopathological findings, lymphatic invasion was identified in 53.33% of the tumors, and the mean mitotic count (MC) was 31.82 ± 26.26. Caveolin-1 showed mild-to-high cytoplasmic expression in neoplastic cells, whereas GATA-3 showed mild-to-high nuclear expression. The Ki67 expression revealed a mean of 24.14 ± 16.88% positive cells. In the univariate analysis, no association was found between each marker and the pathological findings. On the other hand, in multivariate analysis, we identified a positive correlation between GATA-3 and MC and a negative correlation between Caveolin-1 and MC. Moreover, lymphatic invasion was positively correlated with histological type and grade, and negatively correlated with MC. In addition, the histological type was positively correlated with the histological grade. Overall, our results indicate that Caveolin-1 and GATA-3 expression could be promising markers for bladder UC aggressiveness. Frontiers Media S.A. 2022-10-10 /pmc/articles/PMC9589490/ /pubmed/36299636 http://dx.doi.org/10.3389/fvets.2022.986269 Text en Copyright © 2022 Govoni, Pigoli, Brambilla, Ruiz Sueiro, Torres Neto, Laufer-Amorim, Gomes Quitzan, Grieco and Fonseca-Alves. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Govoni, Verônica Mollica
Pigoli, Claudio
Brambilla, Eleonora
Ruiz Sueiro, Felipe Augusto
Torres Neto, Rafael
Laufer-Amorim, Renee
Gomes Quitzan, Juliany
Grieco, Valeria
Fonseca-Alves, Carlos Eduardo
Caveolin-1, GATA-3, and Ki67 expressions and their correlation with pathological findings in canine bladder urothelial carcinoma
title Caveolin-1, GATA-3, and Ki67 expressions and their correlation with pathological findings in canine bladder urothelial carcinoma
title_full Caveolin-1, GATA-3, and Ki67 expressions and their correlation with pathological findings in canine bladder urothelial carcinoma
title_fullStr Caveolin-1, GATA-3, and Ki67 expressions and their correlation with pathological findings in canine bladder urothelial carcinoma
title_full_unstemmed Caveolin-1, GATA-3, and Ki67 expressions and their correlation with pathological findings in canine bladder urothelial carcinoma
title_short Caveolin-1, GATA-3, and Ki67 expressions and their correlation with pathological findings in canine bladder urothelial carcinoma
title_sort caveolin-1, gata-3, and ki67 expressions and their correlation with pathological findings in canine bladder urothelial carcinoma
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9589490/
https://www.ncbi.nlm.nih.gov/pubmed/36299636
http://dx.doi.org/10.3389/fvets.2022.986269
work_keys_str_mv AT govoniveronicamollica caveolin1gata3andki67expressionsandtheircorrelationwithpathologicalfindingsincaninebladderurothelialcarcinoma
AT pigoliclaudio caveolin1gata3andki67expressionsandtheircorrelationwithpathologicalfindingsincaninebladderurothelialcarcinoma
AT brambillaeleonora caveolin1gata3andki67expressionsandtheircorrelationwithpathologicalfindingsincaninebladderurothelialcarcinoma
AT ruizsueirofelipeaugusto caveolin1gata3andki67expressionsandtheircorrelationwithpathologicalfindingsincaninebladderurothelialcarcinoma
AT torresnetorafael caveolin1gata3andki67expressionsandtheircorrelationwithpathologicalfindingsincaninebladderurothelialcarcinoma
AT lauferamorimrenee caveolin1gata3andki67expressionsandtheircorrelationwithpathologicalfindingsincaninebladderurothelialcarcinoma
AT gomesquitzanjuliany caveolin1gata3andki67expressionsandtheircorrelationwithpathologicalfindingsincaninebladderurothelialcarcinoma
AT griecovaleria caveolin1gata3andki67expressionsandtheircorrelationwithpathologicalfindingsincaninebladderurothelialcarcinoma
AT fonsecaalvescarloseduardo caveolin1gata3andki67expressionsandtheircorrelationwithpathologicalfindingsincaninebladderurothelialcarcinoma