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PV-IRES-Cre mouse line targets excitatory granule neurons in the cerebellum
Parvalbumin-expressing inhibitory neurons (PV-INs) are critical for the balance and fine-tuning of complex neuronal circuits. Studies of PV-IN biology require tools for their specific labeling, targeting and manipulation. Among these, the Cre/LoxP system is the most popular in mice, with the two com...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9590163/ https://www.ncbi.nlm.nih.gov/pubmed/36274179 http://dx.doi.org/10.1186/s13041-022-00972-1 |
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author | Wang, Wendy Xueyi Qiao, Julia Lefebvre, Julie L. |
author_facet | Wang, Wendy Xueyi Qiao, Julia Lefebvre, Julie L. |
author_sort | Wang, Wendy Xueyi |
collection | PubMed |
description | Parvalbumin-expressing inhibitory neurons (PV-INs) are critical for the balance and fine-tuning of complex neuronal circuits. Studies of PV-IN biology require tools for their specific labeling, targeting and manipulation. Among these, the Cre/LoxP system is the most popular in mice, with the two commonly used PV-Cre lines cited over 5600 times. Here we report in the mouse cerebellar cortex that PV-Cre activity is not restricted to inhibitory neurons. Imaging of Cre-activated reporters demonstrated recombination in excitatory granule cells. We present evidence that PV-Cre recombination is: (1) spatially regulated and lobule specific; (2) detected in granule cells in the external and internal granule cell layers arising from strong, but transient Pvalb expression in progenitors between E13-E15; and (3) delayed in a subset of inhibitory interneurons, asynchronous with PV protein expression. Together, our findings establish the spatio-temporal patterns PV-Cre activation in the mouse cerebellum, raising considerations for conditional targeting of Pvalb-expressing inhibitory populations. |
format | Online Article Text |
id | pubmed-9590163 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-95901632022-10-25 PV-IRES-Cre mouse line targets excitatory granule neurons in the cerebellum Wang, Wendy Xueyi Qiao, Julia Lefebvre, Julie L. Mol Brain Micro Report Parvalbumin-expressing inhibitory neurons (PV-INs) are critical for the balance and fine-tuning of complex neuronal circuits. Studies of PV-IN biology require tools for their specific labeling, targeting and manipulation. Among these, the Cre/LoxP system is the most popular in mice, with the two commonly used PV-Cre lines cited over 5600 times. Here we report in the mouse cerebellar cortex that PV-Cre activity is not restricted to inhibitory neurons. Imaging of Cre-activated reporters demonstrated recombination in excitatory granule cells. We present evidence that PV-Cre recombination is: (1) spatially regulated and lobule specific; (2) detected in granule cells in the external and internal granule cell layers arising from strong, but transient Pvalb expression in progenitors between E13-E15; and (3) delayed in a subset of inhibitory interneurons, asynchronous with PV protein expression. Together, our findings establish the spatio-temporal patterns PV-Cre activation in the mouse cerebellum, raising considerations for conditional targeting of Pvalb-expressing inhibitory populations. BioMed Central 2022-10-23 /pmc/articles/PMC9590163/ /pubmed/36274179 http://dx.doi.org/10.1186/s13041-022-00972-1 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Micro Report Wang, Wendy Xueyi Qiao, Julia Lefebvre, Julie L. PV-IRES-Cre mouse line targets excitatory granule neurons in the cerebellum |
title | PV-IRES-Cre mouse line targets excitatory granule neurons in the cerebellum |
title_full | PV-IRES-Cre mouse line targets excitatory granule neurons in the cerebellum |
title_fullStr | PV-IRES-Cre mouse line targets excitatory granule neurons in the cerebellum |
title_full_unstemmed | PV-IRES-Cre mouse line targets excitatory granule neurons in the cerebellum |
title_short | PV-IRES-Cre mouse line targets excitatory granule neurons in the cerebellum |
title_sort | pv-ires-cre mouse line targets excitatory granule neurons in the cerebellum |
topic | Micro Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9590163/ https://www.ncbi.nlm.nih.gov/pubmed/36274179 http://dx.doi.org/10.1186/s13041-022-00972-1 |
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