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Amorpha-4,11-diene synthase: a key enzyme in artemisinin biosynthesis and engineering

Amorpha-4,11-diene synthase (ADS) catalyzes the first committed step in the artemisinin biosynthetic pathway, which is the first catalytic reaction enzymatically and genetically characterized in artemisinin biosynthesis. The advent of ADS in Artemisia annua is considered crucial for the emergence of...

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Detalles Bibliográficos
Autores principales: Huang, Jin Quan, Fang, Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Nature Singapore 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9590458/
https://www.ncbi.nlm.nih.gov/pubmed/36303880
http://dx.doi.org/10.1007/s42994-021-00058-x
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author Huang, Jin Quan
Fang, Xin
author_facet Huang, Jin Quan
Fang, Xin
author_sort Huang, Jin Quan
collection PubMed
description Amorpha-4,11-diene synthase (ADS) catalyzes the first committed step in the artemisinin biosynthetic pathway, which is the first catalytic reaction enzymatically and genetically characterized in artemisinin biosynthesis. The advent of ADS in Artemisia annua is considered crucial for the emergence of the specialized artemisinin biosynthetic pathway in the species. Microbial production of amorpha-4,11-diene is a breakthrough in metabolic engineering and synthetic biology. Recently, numerous new techniques have been used in ADS engineering; for example, assessing the substrate promiscuity of ADS to chemoenzymatically produce artemisinin. In this review, we discuss the discovery and catalytic mechanism of ADS, its application in metabolic engineering and synthetic biology, as well as the role of sesquiterpene synthases in the evolutionary origin of artemisinin.
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spelling pubmed-95904582022-10-26 Amorpha-4,11-diene synthase: a key enzyme in artemisinin biosynthesis and engineering Huang, Jin Quan Fang, Xin aBIOTECH Review Amorpha-4,11-diene synthase (ADS) catalyzes the first committed step in the artemisinin biosynthetic pathway, which is the first catalytic reaction enzymatically and genetically characterized in artemisinin biosynthesis. The advent of ADS in Artemisia annua is considered crucial for the emergence of the specialized artemisinin biosynthetic pathway in the species. Microbial production of amorpha-4,11-diene is a breakthrough in metabolic engineering and synthetic biology. Recently, numerous new techniques have been used in ADS engineering; for example, assessing the substrate promiscuity of ADS to chemoenzymatically produce artemisinin. In this review, we discuss the discovery and catalytic mechanism of ADS, its application in metabolic engineering and synthetic biology, as well as the role of sesquiterpene synthases in the evolutionary origin of artemisinin. Springer Nature Singapore 2021-07-30 /pmc/articles/PMC9590458/ /pubmed/36303880 http://dx.doi.org/10.1007/s42994-021-00058-x Text en © The Author(s) 2021, corrected publication 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Review
Huang, Jin Quan
Fang, Xin
Amorpha-4,11-diene synthase: a key enzyme in artemisinin biosynthesis and engineering
title Amorpha-4,11-diene synthase: a key enzyme in artemisinin biosynthesis and engineering
title_full Amorpha-4,11-diene synthase: a key enzyme in artemisinin biosynthesis and engineering
title_fullStr Amorpha-4,11-diene synthase: a key enzyme in artemisinin biosynthesis and engineering
title_full_unstemmed Amorpha-4,11-diene synthase: a key enzyme in artemisinin biosynthesis and engineering
title_short Amorpha-4,11-diene synthase: a key enzyme in artemisinin biosynthesis and engineering
title_sort amorpha-4,11-diene synthase: a key enzyme in artemisinin biosynthesis and engineering
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9590458/
https://www.ncbi.nlm.nih.gov/pubmed/36303880
http://dx.doi.org/10.1007/s42994-021-00058-x
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