Cargando…

Exploration of the Protective Mechanism of Bax Removal against Ischemia Reperfusion Injury of Skin Flap through the p38 Mitogen-Activated Protein Kinase Pathway

This research is aimed at exploring the influences of the Bax gene in the p38 mitogen-activated protein kinase (MAPK) pathway and its protective mechanism against ischemia-reperfusion injury (IRI) of skin flap. Forty male Sprague-Dawley (SD) rats were equally divided into the experimental group (Bax...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Yapeng, Wu, Yongwei, Wang, Peng, Luo, Junhao, Rui, Yongjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9592197/
https://www.ncbi.nlm.nih.gov/pubmed/36299606
http://dx.doi.org/10.1155/2022/1175078
_version_ 1784814868910571520
author Wang, Yapeng
Wu, Yongwei
Wang, Peng
Luo, Junhao
Rui, Yongjun
author_facet Wang, Yapeng
Wu, Yongwei
Wang, Peng
Luo, Junhao
Rui, Yongjun
author_sort Wang, Yapeng
collection PubMed
description This research is aimed at exploring the influences of the Bax gene in the p38 mitogen-activated protein kinase (MAPK) pathway and its protective mechanism against ischemia-reperfusion injury (IRI) of skin flap. Forty male Sprague-Dawley (SD) rats were equally divided into the experimental group (Bax gene knockout rats) and control group. The dorsal flap model was prepared, and the survival rate of flap was observed after surgery. The rat flap tissue was cut and stained with hematoxylin-eosin (HE) and in situ terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL). The distribution characteristics of p38MAPK and Bax were detected to evaluate the protective mechanism of Bax gene knockout on IRI of skin flap. After surgery, the survival rate of flaps in the experimental group (82.32%, 70.28%) was significantly higher than that in the control group (57.64%, 46.14%) (P < 0.05). The results of HE staining showed that on the 1(st) day after surgery, compared with those in the control group, the skin flaps of the rats in the experimental group were arranged more neatly. The results of TUNEL staining showed that compared with that of the control group, the tissue structure of the skin flap of the experimental group was normal and only a few apoptotic cells appeared. In addition, compared with that in the control group (7.14, 4.25, 3.48, 2.18/6.46, 7.12, 4.86, and 2.44), the expression of Bax and p38 MAPK in the experimental group (0.96, 0.81, 0.76, 0.55/1.63, 1.33, 1.01, and 0.56) significantly decreased (P < 0.05). In short, after the Bax gene was knocked out, injury of the flap after ischemia-reperfusion was considerably improved, which may play a protective role on the IRI of the flap by affecting the p38MAPK pathway.
format Online
Article
Text
id pubmed-9592197
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-95921972022-10-25 Exploration of the Protective Mechanism of Bax Removal against Ischemia Reperfusion Injury of Skin Flap through the p38 Mitogen-Activated Protein Kinase Pathway Wang, Yapeng Wu, Yongwei Wang, Peng Luo, Junhao Rui, Yongjun Oxid Med Cell Longev Research Article This research is aimed at exploring the influences of the Bax gene in the p38 mitogen-activated protein kinase (MAPK) pathway and its protective mechanism against ischemia-reperfusion injury (IRI) of skin flap. Forty male Sprague-Dawley (SD) rats were equally divided into the experimental group (Bax gene knockout rats) and control group. The dorsal flap model was prepared, and the survival rate of flap was observed after surgery. The rat flap tissue was cut and stained with hematoxylin-eosin (HE) and in situ terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL). The distribution characteristics of p38MAPK and Bax were detected to evaluate the protective mechanism of Bax gene knockout on IRI of skin flap. After surgery, the survival rate of flaps in the experimental group (82.32%, 70.28%) was significantly higher than that in the control group (57.64%, 46.14%) (P < 0.05). The results of HE staining showed that on the 1(st) day after surgery, compared with those in the control group, the skin flaps of the rats in the experimental group were arranged more neatly. The results of TUNEL staining showed that compared with that of the control group, the tissue structure of the skin flap of the experimental group was normal and only a few apoptotic cells appeared. In addition, compared with that in the control group (7.14, 4.25, 3.48, 2.18/6.46, 7.12, 4.86, and 2.44), the expression of Bax and p38 MAPK in the experimental group (0.96, 0.81, 0.76, 0.55/1.63, 1.33, 1.01, and 0.56) significantly decreased (P < 0.05). In short, after the Bax gene was knocked out, injury of the flap after ischemia-reperfusion was considerably improved, which may play a protective role on the IRI of the flap by affecting the p38MAPK pathway. Hindawi 2022-10-17 /pmc/articles/PMC9592197/ /pubmed/36299606 http://dx.doi.org/10.1155/2022/1175078 Text en Copyright © 2022 Yapeng Wang et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Wang, Yapeng
Wu, Yongwei
Wang, Peng
Luo, Junhao
Rui, Yongjun
Exploration of the Protective Mechanism of Bax Removal against Ischemia Reperfusion Injury of Skin Flap through the p38 Mitogen-Activated Protein Kinase Pathway
title Exploration of the Protective Mechanism of Bax Removal against Ischemia Reperfusion Injury of Skin Flap through the p38 Mitogen-Activated Protein Kinase Pathway
title_full Exploration of the Protective Mechanism of Bax Removal against Ischemia Reperfusion Injury of Skin Flap through the p38 Mitogen-Activated Protein Kinase Pathway
title_fullStr Exploration of the Protective Mechanism of Bax Removal against Ischemia Reperfusion Injury of Skin Flap through the p38 Mitogen-Activated Protein Kinase Pathway
title_full_unstemmed Exploration of the Protective Mechanism of Bax Removal against Ischemia Reperfusion Injury of Skin Flap through the p38 Mitogen-Activated Protein Kinase Pathway
title_short Exploration of the Protective Mechanism of Bax Removal against Ischemia Reperfusion Injury of Skin Flap through the p38 Mitogen-Activated Protein Kinase Pathway
title_sort exploration of the protective mechanism of bax removal against ischemia reperfusion injury of skin flap through the p38 mitogen-activated protein kinase pathway
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9592197/
https://www.ncbi.nlm.nih.gov/pubmed/36299606
http://dx.doi.org/10.1155/2022/1175078
work_keys_str_mv AT wangyapeng explorationoftheprotectivemechanismofbaxremovalagainstischemiareperfusioninjuryofskinflapthroughthep38mitogenactivatedproteinkinasepathway
AT wuyongwei explorationoftheprotectivemechanismofbaxremovalagainstischemiareperfusioninjuryofskinflapthroughthep38mitogenactivatedproteinkinasepathway
AT wangpeng explorationoftheprotectivemechanismofbaxremovalagainstischemiareperfusioninjuryofskinflapthroughthep38mitogenactivatedproteinkinasepathway
AT luojunhao explorationoftheprotectivemechanismofbaxremovalagainstischemiareperfusioninjuryofskinflapthroughthep38mitogenactivatedproteinkinasepathway
AT ruiyongjun explorationoftheprotectivemechanismofbaxremovalagainstischemiareperfusioninjuryofskinflapthroughthep38mitogenactivatedproteinkinasepathway