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Development of a Nanostructured Electrochemical Genosensor for the Detection of the K-ras Gene

In the scientific literature, it has been documented that electrochemical genosensors are novel analytical tools with proven clinical diagnostic potential for the identification of carcinogenic processes due to genetic and epigenetic alterations, as well as infectious diseases due to viruses or bact...

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Autores principales: Garcia-Melo, Luis Fernando, Morales-Rodríguez, Miguel, Madrigal-Bujaidar, Eduardo, Madrigal-Santillán, Eduardo O., Morales-González, José Antonio, Pineda Cruces, Rosa Natali, Campoy Ramírez, Jorge Alfredo, Damian-Matsumura, Pablo, Tellez-Plancarte, Alexandro, Batina, Nikola, Álvarez-González, Isela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9592225/
https://www.ncbi.nlm.nih.gov/pubmed/36299712
http://dx.doi.org/10.1155/2022/6575140
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author Garcia-Melo, Luis Fernando
Morales-Rodríguez, Miguel
Madrigal-Bujaidar, Eduardo
Madrigal-Santillán, Eduardo O.
Morales-González, José Antonio
Pineda Cruces, Rosa Natali
Campoy Ramírez, Jorge Alfredo
Damian-Matsumura, Pablo
Tellez-Plancarte, Alexandro
Batina, Nikola
Álvarez-González, Isela
author_facet Garcia-Melo, Luis Fernando
Morales-Rodríguez, Miguel
Madrigal-Bujaidar, Eduardo
Madrigal-Santillán, Eduardo O.
Morales-González, José Antonio
Pineda Cruces, Rosa Natali
Campoy Ramírez, Jorge Alfredo
Damian-Matsumura, Pablo
Tellez-Plancarte, Alexandro
Batina, Nikola
Álvarez-González, Isela
author_sort Garcia-Melo, Luis Fernando
collection PubMed
description In the scientific literature, it has been documented that electrochemical genosensors are novel analytical tools with proven clinical diagnostic potential for the identification of carcinogenic processes due to genetic and epigenetic alterations, as well as infectious diseases due to viruses or bacteria. In the present work, we describe the construction of an electrochemical genosensor for the identification of the k12p.1 mutation; it was based on use of Screen-Printed Gold Electrode (SPGE), Cyclic Voltammetry (CV), and Atomic Force Microscopy (AFM), for the monitoring the electron transfer trough the functionalized nanostructured surface and corresponding morphological changes. The sensitivity of the genosensor showed a linear response for the identification of the k12p.1 mutation of the K-ras gene in the concentration range of 10 fM to 1 μM with a detection limit of 7.96 fM in the presence of doxorubicin (Dox) as DNA intercalating agent and indicator of the hybridization reaction. Thus, the electrochemical genosensor developed could be useful for the identification of diseases related with the K-ras oncogene.
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spelling pubmed-95922252022-10-25 Development of a Nanostructured Electrochemical Genosensor for the Detection of the K-ras Gene Garcia-Melo, Luis Fernando Morales-Rodríguez, Miguel Madrigal-Bujaidar, Eduardo Madrigal-Santillán, Eduardo O. Morales-González, José Antonio Pineda Cruces, Rosa Natali Campoy Ramírez, Jorge Alfredo Damian-Matsumura, Pablo Tellez-Plancarte, Alexandro Batina, Nikola Álvarez-González, Isela J Anal Methods Chem Research Article In the scientific literature, it has been documented that electrochemical genosensors are novel analytical tools with proven clinical diagnostic potential for the identification of carcinogenic processes due to genetic and epigenetic alterations, as well as infectious diseases due to viruses or bacteria. In the present work, we describe the construction of an electrochemical genosensor for the identification of the k12p.1 mutation; it was based on use of Screen-Printed Gold Electrode (SPGE), Cyclic Voltammetry (CV), and Atomic Force Microscopy (AFM), for the monitoring the electron transfer trough the functionalized nanostructured surface and corresponding morphological changes. The sensitivity of the genosensor showed a linear response for the identification of the k12p.1 mutation of the K-ras gene in the concentration range of 10 fM to 1 μM with a detection limit of 7.96 fM in the presence of doxorubicin (Dox) as DNA intercalating agent and indicator of the hybridization reaction. Thus, the electrochemical genosensor developed could be useful for the identification of diseases related with the K-ras oncogene. Hindawi 2022-10-17 /pmc/articles/PMC9592225/ /pubmed/36299712 http://dx.doi.org/10.1155/2022/6575140 Text en Copyright © 2022 Luis Fernando Garcia-Melo et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Garcia-Melo, Luis Fernando
Morales-Rodríguez, Miguel
Madrigal-Bujaidar, Eduardo
Madrigal-Santillán, Eduardo O.
Morales-González, José Antonio
Pineda Cruces, Rosa Natali
Campoy Ramírez, Jorge Alfredo
Damian-Matsumura, Pablo
Tellez-Plancarte, Alexandro
Batina, Nikola
Álvarez-González, Isela
Development of a Nanostructured Electrochemical Genosensor for the Detection of the K-ras Gene
title Development of a Nanostructured Electrochemical Genosensor for the Detection of the K-ras Gene
title_full Development of a Nanostructured Electrochemical Genosensor for the Detection of the K-ras Gene
title_fullStr Development of a Nanostructured Electrochemical Genosensor for the Detection of the K-ras Gene
title_full_unstemmed Development of a Nanostructured Electrochemical Genosensor for the Detection of the K-ras Gene
title_short Development of a Nanostructured Electrochemical Genosensor for the Detection of the K-ras Gene
title_sort development of a nanostructured electrochemical genosensor for the detection of the k-ras gene
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9592225/
https://www.ncbi.nlm.nih.gov/pubmed/36299712
http://dx.doi.org/10.1155/2022/6575140
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