Cargando…
Development of a Nanostructured Electrochemical Genosensor for the Detection of the K-ras Gene
In the scientific literature, it has been documented that electrochemical genosensors are novel analytical tools with proven clinical diagnostic potential for the identification of carcinogenic processes due to genetic and epigenetic alterations, as well as infectious diseases due to viruses or bact...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9592225/ https://www.ncbi.nlm.nih.gov/pubmed/36299712 http://dx.doi.org/10.1155/2022/6575140 |
_version_ | 1784814875700101120 |
---|---|
author | Garcia-Melo, Luis Fernando Morales-Rodríguez, Miguel Madrigal-Bujaidar, Eduardo Madrigal-Santillán, Eduardo O. Morales-González, José Antonio Pineda Cruces, Rosa Natali Campoy Ramírez, Jorge Alfredo Damian-Matsumura, Pablo Tellez-Plancarte, Alexandro Batina, Nikola Álvarez-González, Isela |
author_facet | Garcia-Melo, Luis Fernando Morales-Rodríguez, Miguel Madrigal-Bujaidar, Eduardo Madrigal-Santillán, Eduardo O. Morales-González, José Antonio Pineda Cruces, Rosa Natali Campoy Ramírez, Jorge Alfredo Damian-Matsumura, Pablo Tellez-Plancarte, Alexandro Batina, Nikola Álvarez-González, Isela |
author_sort | Garcia-Melo, Luis Fernando |
collection | PubMed |
description | In the scientific literature, it has been documented that electrochemical genosensors are novel analytical tools with proven clinical diagnostic potential for the identification of carcinogenic processes due to genetic and epigenetic alterations, as well as infectious diseases due to viruses or bacteria. In the present work, we describe the construction of an electrochemical genosensor for the identification of the k12p.1 mutation; it was based on use of Screen-Printed Gold Electrode (SPGE), Cyclic Voltammetry (CV), and Atomic Force Microscopy (AFM), for the monitoring the electron transfer trough the functionalized nanostructured surface and corresponding morphological changes. The sensitivity of the genosensor showed a linear response for the identification of the k12p.1 mutation of the K-ras gene in the concentration range of 10 fM to 1 μM with a detection limit of 7.96 fM in the presence of doxorubicin (Dox) as DNA intercalating agent and indicator of the hybridization reaction. Thus, the electrochemical genosensor developed could be useful for the identification of diseases related with the K-ras oncogene. |
format | Online Article Text |
id | pubmed-9592225 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-95922252022-10-25 Development of a Nanostructured Electrochemical Genosensor for the Detection of the K-ras Gene Garcia-Melo, Luis Fernando Morales-Rodríguez, Miguel Madrigal-Bujaidar, Eduardo Madrigal-Santillán, Eduardo O. Morales-González, José Antonio Pineda Cruces, Rosa Natali Campoy Ramírez, Jorge Alfredo Damian-Matsumura, Pablo Tellez-Plancarte, Alexandro Batina, Nikola Álvarez-González, Isela J Anal Methods Chem Research Article In the scientific literature, it has been documented that electrochemical genosensors are novel analytical tools with proven clinical diagnostic potential for the identification of carcinogenic processes due to genetic and epigenetic alterations, as well as infectious diseases due to viruses or bacteria. In the present work, we describe the construction of an electrochemical genosensor for the identification of the k12p.1 mutation; it was based on use of Screen-Printed Gold Electrode (SPGE), Cyclic Voltammetry (CV), and Atomic Force Microscopy (AFM), for the monitoring the electron transfer trough the functionalized nanostructured surface and corresponding morphological changes. The sensitivity of the genosensor showed a linear response for the identification of the k12p.1 mutation of the K-ras gene in the concentration range of 10 fM to 1 μM with a detection limit of 7.96 fM in the presence of doxorubicin (Dox) as DNA intercalating agent and indicator of the hybridization reaction. Thus, the electrochemical genosensor developed could be useful for the identification of diseases related with the K-ras oncogene. Hindawi 2022-10-17 /pmc/articles/PMC9592225/ /pubmed/36299712 http://dx.doi.org/10.1155/2022/6575140 Text en Copyright © 2022 Luis Fernando Garcia-Melo et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Garcia-Melo, Luis Fernando Morales-Rodríguez, Miguel Madrigal-Bujaidar, Eduardo Madrigal-Santillán, Eduardo O. Morales-González, José Antonio Pineda Cruces, Rosa Natali Campoy Ramírez, Jorge Alfredo Damian-Matsumura, Pablo Tellez-Plancarte, Alexandro Batina, Nikola Álvarez-González, Isela Development of a Nanostructured Electrochemical Genosensor for the Detection of the K-ras Gene |
title | Development of a Nanostructured Electrochemical Genosensor for the Detection of the K-ras Gene |
title_full | Development of a Nanostructured Electrochemical Genosensor for the Detection of the K-ras Gene |
title_fullStr | Development of a Nanostructured Electrochemical Genosensor for the Detection of the K-ras Gene |
title_full_unstemmed | Development of a Nanostructured Electrochemical Genosensor for the Detection of the K-ras Gene |
title_short | Development of a Nanostructured Electrochemical Genosensor for the Detection of the K-ras Gene |
title_sort | development of a nanostructured electrochemical genosensor for the detection of the k-ras gene |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9592225/ https://www.ncbi.nlm.nih.gov/pubmed/36299712 http://dx.doi.org/10.1155/2022/6575140 |
work_keys_str_mv | AT garciameloluisfernando developmentofananostructuredelectrochemicalgenosensorforthedetectionofthekrasgene AT moralesrodriguezmiguel developmentofananostructuredelectrochemicalgenosensorforthedetectionofthekrasgene AT madrigalbujaidareduardo developmentofananostructuredelectrochemicalgenosensorforthedetectionofthekrasgene AT madrigalsantillaneduardoo developmentofananostructuredelectrochemicalgenosensorforthedetectionofthekrasgene AT moralesgonzalezjoseantonio developmentofananostructuredelectrochemicalgenosensorforthedetectionofthekrasgene AT pinedacrucesrosanatali developmentofananostructuredelectrochemicalgenosensorforthedetectionofthekrasgene AT campoyramirezjorgealfredo developmentofananostructuredelectrochemicalgenosensorforthedetectionofthekrasgene AT damianmatsumurapablo developmentofananostructuredelectrochemicalgenosensorforthedetectionofthekrasgene AT tellezplancartealexandro developmentofananostructuredelectrochemicalgenosensorforthedetectionofthekrasgene AT batinanikola developmentofananostructuredelectrochemicalgenosensorforthedetectionofthekrasgene AT alvarezgonzalezisela developmentofananostructuredelectrochemicalgenosensorforthedetectionofthekrasgene |