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Interplay among cell migration, shaping, and traction force on a matrix with cell-scale stiffness heterogeneity

In living tissues where cells migrate, the spatial distribution of mechanical properties, especially matrix stiffness, is generally heterogeneous, with cell scales ranging from 10 to 1000 μm. Since cell migration in the body plays a critical role in morphogenesis, wound healing, and cancer metastasi...

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Detalles Bibliográficos
Autores principales: Ebata, Hiroyuki, Kidoaki, Satoru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Biophysical Society of Japan 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9592569/
https://www.ncbi.nlm.nih.gov/pubmed/36349327
http://dx.doi.org/10.2142/biophysico.bppb-v19.0036
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author Ebata, Hiroyuki
Kidoaki, Satoru
author_facet Ebata, Hiroyuki
Kidoaki, Satoru
author_sort Ebata, Hiroyuki
collection PubMed
description In living tissues where cells migrate, the spatial distribution of mechanical properties, especially matrix stiffness, is generally heterogeneous, with cell scales ranging from 10 to 1000 μm. Since cell migration in the body plays a critical role in morphogenesis, wound healing, and cancer metastasis, it is essential to understand the migratory dynamics on the matrix with cell-scale stiffness heterogeneity. In general, cell migration is driven by the extension and contraction of the cell body owing to the force from actin polymerization and myosin motors in the actomyosin cytoskeleton. When a cell is placed on a matrix with a simple stiffness gradient, directional migration called durotaxis emerges because of the asymmetric extension and contraction of the pseudopodia, which is accompanied by the asymmetric distribution of focal adhesions. Similarly, to determine cell migration on a matrix with cell-scale stiffness heterogeneity, the interaction between cell-scale stiffness heterogeneity and cellular responses, such as the dynamics of the cell-matrix adhesion site, intracellular prestress, and cell shape, should play a key role. In this review, we summarize systematic studies on the dynamics of cell migration, shaping, and traction force on a matrix with cell-scale stiffness heterogeneity using micro-elastically patterned hydrogels. We also outline the cell migration model based on cell-shaping dynamics that explains the general durotaxis induced by cell-scale stiffness heterogeneity. This review article is an extended version of the Japanese article, Dynamics of Cell Shaping and Migration on the Matrix with Cell-scale Stiffness-heterogeneity, published in SEIBUTSU BUTSURI Vol. 61, p. 152–156 (2021).
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spelling pubmed-95925692022-11-07 Interplay among cell migration, shaping, and traction force on a matrix with cell-scale stiffness heterogeneity Ebata, Hiroyuki Kidoaki, Satoru Biophys Physicobiol Review Article (Invited) In living tissues where cells migrate, the spatial distribution of mechanical properties, especially matrix stiffness, is generally heterogeneous, with cell scales ranging from 10 to 1000 μm. Since cell migration in the body plays a critical role in morphogenesis, wound healing, and cancer metastasis, it is essential to understand the migratory dynamics on the matrix with cell-scale stiffness heterogeneity. In general, cell migration is driven by the extension and contraction of the cell body owing to the force from actin polymerization and myosin motors in the actomyosin cytoskeleton. When a cell is placed on a matrix with a simple stiffness gradient, directional migration called durotaxis emerges because of the asymmetric extension and contraction of the pseudopodia, which is accompanied by the asymmetric distribution of focal adhesions. Similarly, to determine cell migration on a matrix with cell-scale stiffness heterogeneity, the interaction between cell-scale stiffness heterogeneity and cellular responses, such as the dynamics of the cell-matrix adhesion site, intracellular prestress, and cell shape, should play a key role. In this review, we summarize systematic studies on the dynamics of cell migration, shaping, and traction force on a matrix with cell-scale stiffness heterogeneity using micro-elastically patterned hydrogels. We also outline the cell migration model based on cell-shaping dynamics that explains the general durotaxis induced by cell-scale stiffness heterogeneity. This review article is an extended version of the Japanese article, Dynamics of Cell Shaping and Migration on the Matrix with Cell-scale Stiffness-heterogeneity, published in SEIBUTSU BUTSURI Vol. 61, p. 152–156 (2021). The Biophysical Society of Japan 2022-09-13 /pmc/articles/PMC9592569/ /pubmed/36349327 http://dx.doi.org/10.2142/biophysico.bppb-v19.0036 Text en 2022 THE BIOPHYSICAL SOCIETY OF JAPAN https://creativecommons.org/licenses/by-nc-sa/4.0/This article is licensed under the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 Inter­national License. To view a copy of this license, visit 
https://creativecommons.org/licenses/by-nc-sa/4.0/.
spellingShingle Review Article (Invited)
Ebata, Hiroyuki
Kidoaki, Satoru
Interplay among cell migration, shaping, and traction force on a matrix with cell-scale stiffness heterogeneity
title Interplay among cell migration, shaping, and traction force on a matrix with cell-scale stiffness heterogeneity
title_full Interplay among cell migration, shaping, and traction force on a matrix with cell-scale stiffness heterogeneity
title_fullStr Interplay among cell migration, shaping, and traction force on a matrix with cell-scale stiffness heterogeneity
title_full_unstemmed Interplay among cell migration, shaping, and traction force on a matrix with cell-scale stiffness heterogeneity
title_short Interplay among cell migration, shaping, and traction force on a matrix with cell-scale stiffness heterogeneity
title_sort interplay among cell migration, shaping, and traction force on a matrix with cell-scale stiffness heterogeneity
topic Review Article (Invited)
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9592569/
https://www.ncbi.nlm.nih.gov/pubmed/36349327
http://dx.doi.org/10.2142/biophysico.bppb-v19.0036
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