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Interplay among cell migration, shaping, and traction force on a matrix with cell-scale stiffness heterogeneity
In living tissues where cells migrate, the spatial distribution of mechanical properties, especially matrix stiffness, is generally heterogeneous, with cell scales ranging from 10 to 1000 μm. Since cell migration in the body plays a critical role in morphogenesis, wound healing, and cancer metastasi...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Biophysical Society of Japan
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9592569/ https://www.ncbi.nlm.nih.gov/pubmed/36349327 http://dx.doi.org/10.2142/biophysico.bppb-v19.0036 |
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author | Ebata, Hiroyuki Kidoaki, Satoru |
author_facet | Ebata, Hiroyuki Kidoaki, Satoru |
author_sort | Ebata, Hiroyuki |
collection | PubMed |
description | In living tissues where cells migrate, the spatial distribution of mechanical properties, especially matrix stiffness, is generally heterogeneous, with cell scales ranging from 10 to 1000 μm. Since cell migration in the body plays a critical role in morphogenesis, wound healing, and cancer metastasis, it is essential to understand the migratory dynamics on the matrix with cell-scale stiffness heterogeneity. In general, cell migration is driven by the extension and contraction of the cell body owing to the force from actin polymerization and myosin motors in the actomyosin cytoskeleton. When a cell is placed on a matrix with a simple stiffness gradient, directional migration called durotaxis emerges because of the asymmetric extension and contraction of the pseudopodia, which is accompanied by the asymmetric distribution of focal adhesions. Similarly, to determine cell migration on a matrix with cell-scale stiffness heterogeneity, the interaction between cell-scale stiffness heterogeneity and cellular responses, such as the dynamics of the cell-matrix adhesion site, intracellular prestress, and cell shape, should play a key role. In this review, we summarize systematic studies on the dynamics of cell migration, shaping, and traction force on a matrix with cell-scale stiffness heterogeneity using micro-elastically patterned hydrogels. We also outline the cell migration model based on cell-shaping dynamics that explains the general durotaxis induced by cell-scale stiffness heterogeneity. This review article is an extended version of the Japanese article, Dynamics of Cell Shaping and Migration on the Matrix with Cell-scale Stiffness-heterogeneity, published in SEIBUTSU BUTSURI Vol. 61, p. 152–156 (2021). |
format | Online Article Text |
id | pubmed-9592569 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | The Biophysical Society of Japan |
record_format | MEDLINE/PubMed |
spelling | pubmed-95925692022-11-07 Interplay among cell migration, shaping, and traction force on a matrix with cell-scale stiffness heterogeneity Ebata, Hiroyuki Kidoaki, Satoru Biophys Physicobiol Review Article (Invited) In living tissues where cells migrate, the spatial distribution of mechanical properties, especially matrix stiffness, is generally heterogeneous, with cell scales ranging from 10 to 1000 μm. Since cell migration in the body plays a critical role in morphogenesis, wound healing, and cancer metastasis, it is essential to understand the migratory dynamics on the matrix with cell-scale stiffness heterogeneity. In general, cell migration is driven by the extension and contraction of the cell body owing to the force from actin polymerization and myosin motors in the actomyosin cytoskeleton. When a cell is placed on a matrix with a simple stiffness gradient, directional migration called durotaxis emerges because of the asymmetric extension and contraction of the pseudopodia, which is accompanied by the asymmetric distribution of focal adhesions. Similarly, to determine cell migration on a matrix with cell-scale stiffness heterogeneity, the interaction between cell-scale stiffness heterogeneity and cellular responses, such as the dynamics of the cell-matrix adhesion site, intracellular prestress, and cell shape, should play a key role. In this review, we summarize systematic studies on the dynamics of cell migration, shaping, and traction force on a matrix with cell-scale stiffness heterogeneity using micro-elastically patterned hydrogels. We also outline the cell migration model based on cell-shaping dynamics that explains the general durotaxis induced by cell-scale stiffness heterogeneity. This review article is an extended version of the Japanese article, Dynamics of Cell Shaping and Migration on the Matrix with Cell-scale Stiffness-heterogeneity, published in SEIBUTSU BUTSURI Vol. 61, p. 152–156 (2021). The Biophysical Society of Japan 2022-09-13 /pmc/articles/PMC9592569/ /pubmed/36349327 http://dx.doi.org/10.2142/biophysico.bppb-v19.0036 Text en 2022 THE BIOPHYSICAL SOCIETY OF JAPAN https://creativecommons.org/licenses/by-nc-sa/4.0/This article is licensed under the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. To view a copy of this license, visit
https://creativecommons.org/licenses/by-nc-sa/4.0/. |
spellingShingle | Review Article (Invited) Ebata, Hiroyuki Kidoaki, Satoru Interplay among cell migration, shaping, and traction force on a matrix with cell-scale stiffness heterogeneity |
title | Interplay among cell migration, shaping, and traction force on a matrix with cell-scale stiffness heterogeneity |
title_full | Interplay among cell migration, shaping, and traction force on a matrix with cell-scale stiffness heterogeneity |
title_fullStr | Interplay among cell migration, shaping, and traction force on a matrix with cell-scale stiffness heterogeneity |
title_full_unstemmed | Interplay among cell migration, shaping, and traction force on a matrix with cell-scale stiffness heterogeneity |
title_short | Interplay among cell migration, shaping, and traction force on a matrix with cell-scale stiffness heterogeneity |
title_sort | interplay among cell migration, shaping, and traction force on a matrix with cell-scale stiffness heterogeneity |
topic | Review Article (Invited) |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9592569/ https://www.ncbi.nlm.nih.gov/pubmed/36349327 http://dx.doi.org/10.2142/biophysico.bppb-v19.0036 |
work_keys_str_mv | AT ebatahiroyuki interplayamongcellmigrationshapingandtractionforceonamatrixwithcellscalestiffnessheterogeneity AT kidoakisatoru interplayamongcellmigrationshapingandtractionforceonamatrixwithcellscalestiffnessheterogeneity |