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Influence of gap junctions upon Ca(2+) propagation from stimulated keratinocytes to DRG neurons
Epidermal cells, such as keratinocytes, are regarded as the first sensory cells to transmit nociception and mechanoreception to free nerve endings extended from the dorsal root ganglion (DRG). Previous studies suggested that this transmission occurs as Ca(2+) propagation via ATP receptors. Conversel...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Biophysical Society of Japan
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9592570/ https://www.ncbi.nlm.nih.gov/pubmed/36349331 http://dx.doi.org/10.2142/biophysico.bppb-v19.0041 |
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author | Seto, Chiaki Toyoda, Kenta Inada, Kousuke Oka, Kotaro Ito, Etsuro |
author_facet | Seto, Chiaki Toyoda, Kenta Inada, Kousuke Oka, Kotaro Ito, Etsuro |
author_sort | Seto, Chiaki |
collection | PubMed |
description | Epidermal cells, such as keratinocytes, are regarded as the first sensory cells to transmit nociception and mechanoreception to free nerve endings extended from the dorsal root ganglion (DRG). Previous studies suggested that this transmission occurs as Ca(2+) propagation via ATP receptors. Conversely, the influence of gap junctions on this Ca(2+) propagation is largely unknown. Thus, we examined the localization and the role of connexin 43 among keratinocytes and DRG neurons. We co-cultured keratinocytes and DRG neurons and investigated the effect of pharmacological blockade of gap junctions on Ca(2+) propagation upon stimulation of a single keratinocyte. Immunocytochemical experiments showed that connexin 43 is localized between keratinocytes and between keratinocytes and DRG neurons. Octanol, a gap junction inhibitor, significantly suppressed the concentrical Ca(2+) propagation. Therefore, we conclude that the Ca(2+) propagation mechanism via gap junctions from stimulated keratinocytes to free nerve endings should be taken into account. |
format | Online Article Text |
id | pubmed-9592570 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | The Biophysical Society of Japan |
record_format | MEDLINE/PubMed |
spelling | pubmed-95925702022-11-07 Influence of gap junctions upon Ca(2+) propagation from stimulated keratinocytes to DRG neurons Seto, Chiaki Toyoda, Kenta Inada, Kousuke Oka, Kotaro Ito, Etsuro Biophys Physicobiol Regular Article Epidermal cells, such as keratinocytes, are regarded as the first sensory cells to transmit nociception and mechanoreception to free nerve endings extended from the dorsal root ganglion (DRG). Previous studies suggested that this transmission occurs as Ca(2+) propagation via ATP receptors. Conversely, the influence of gap junctions on this Ca(2+) propagation is largely unknown. Thus, we examined the localization and the role of connexin 43 among keratinocytes and DRG neurons. We co-cultured keratinocytes and DRG neurons and investigated the effect of pharmacological blockade of gap junctions on Ca(2+) propagation upon stimulation of a single keratinocyte. Immunocytochemical experiments showed that connexin 43 is localized between keratinocytes and between keratinocytes and DRG neurons. Octanol, a gap junction inhibitor, significantly suppressed the concentrical Ca(2+) propagation. Therefore, we conclude that the Ca(2+) propagation mechanism via gap junctions from stimulated keratinocytes to free nerve endings should be taken into account. The Biophysical Society of Japan 2022-09-22 /pmc/articles/PMC9592570/ /pubmed/36349331 http://dx.doi.org/10.2142/biophysico.bppb-v19.0041 Text en 2022 THE BIOPHYSICAL SOCIETY OF JAPAN https://creativecommons.org/licenses/by-nc-sa/4.0/This article is licensed under the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. To view a copy of this license, visit
https://creativecommons.org/licenses/by-nc-sa/4.0/. |
spellingShingle | Regular Article Seto, Chiaki Toyoda, Kenta Inada, Kousuke Oka, Kotaro Ito, Etsuro Influence of gap junctions upon Ca(2+) propagation from stimulated keratinocytes to DRG neurons |
title | Influence of gap junctions upon Ca(2+) propagation from stimulated keratinocytes to DRG neurons |
title_full | Influence of gap junctions upon Ca(2+) propagation from stimulated keratinocytes to DRG neurons |
title_fullStr | Influence of gap junctions upon Ca(2+) propagation from stimulated keratinocytes to DRG neurons |
title_full_unstemmed | Influence of gap junctions upon Ca(2+) propagation from stimulated keratinocytes to DRG neurons |
title_short | Influence of gap junctions upon Ca(2+) propagation from stimulated keratinocytes to DRG neurons |
title_sort | influence of gap junctions upon ca(2+) propagation from stimulated keratinocytes to drg neurons |
topic | Regular Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9592570/ https://www.ncbi.nlm.nih.gov/pubmed/36349331 http://dx.doi.org/10.2142/biophysico.bppb-v19.0041 |
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