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Improved site-specific mutagenesis in Rhodococcus opacus using a novel conditional suicide plasmid
ABSTRACT: Rhodococcus opacus PD630 is a biotechnologically important bacterium with metabolic capability for bioremediation, metal recovery, and storage of triacylglycerols. Genome editing by homologous recombination in R. opacus is hampered by a very low combined frequency of DNA transfer and recom...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9592669/ https://www.ncbi.nlm.nih.gov/pubmed/36194264 http://dx.doi.org/10.1007/s00253-022-12204-6 |