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Improved site-specific mutagenesis in Rhodococcus opacus using a novel conditional suicide plasmid

ABSTRACT: Rhodococcus opacus PD630 is a biotechnologically important bacterium with metabolic capability for bioremediation, metal recovery, and storage of triacylglycerols. Genome editing by homologous recombination in R. opacus is hampered by a very low combined frequency of DNA transfer and recom...

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Detalles Bibliográficos
Autores principales:	Jain, Garima, Ertesvåg, Helga
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Springer Berlin Heidelberg 2022
Materias:	Applied Genetics and Molecular Biotechnology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9592669/ https://www.ncbi.nlm.nih.gov/pubmed/36194264 http://dx.doi.org/10.1007/s00253-022-12204-6

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