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Rhinolophus sinicus virome revealed multiple novel mosquito-borne zoonotic viruses
To exploit the Rhinolophus sinicus–specific virome, 29 Rhinolophus sinicus were gathered in Lincang, China. Enriched viral sequences of 22 virus families were acquired by metavirome techniques. Hereby, the part of virome in Rhinolophus sinicus, including Chikungunya virus (CHIKV), Getah virus, and J...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9592836/ https://www.ncbi.nlm.nih.gov/pubmed/36304937 http://dx.doi.org/10.3389/fcimb.2022.960507 |
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author | Peng, Chengcheng Zhang, Duo Li, Chenghui Li, Yiquan Zhang, He Li, Nan Xiao, Pengpeng |
author_facet | Peng, Chengcheng Zhang, Duo Li, Chenghui Li, Yiquan Zhang, He Li, Nan Xiao, Pengpeng |
author_sort | Peng, Chengcheng |
collection | PubMed |
description | To exploit the Rhinolophus sinicus–specific virome, 29 Rhinolophus sinicus were gathered in Lincang, China. Enriched viral sequences of 22 virus families were acquired by metavirome techniques. Hereby, the part of virome in Rhinolophus sinicus, including Chikungunya virus (CHIKV), Getah virus, and Japanese encephalitis virus (JEV) were validated by PCR. Five CHIKV viral sequences were amplified, among which CHIKV-China/B2016C-1 shared the highest homology to CHIKV isolated from Italy in 2007, with the genotype as African ECS. Eight JEV viral sequences were amplified, of which JEV-China/B2016E-1 shared the highest homology with at least 91.3% nt identity with the JEV sequence found in South Korea in 1988 and was classified as genotype III. Notably, JEV was isolated for the first time in Rhinolophus sinicus. The newly isolated JEV-China/B2016-1 could increase infectivity while passaging in Vero cells from BHK-21 cells. Overall, the research sheds insight into the diversity and viral susceptibility dynamics of the virome in Rhinolophus sinicus and reveals new light on the ecology of other important viral hosts. |
format | Online Article Text |
id | pubmed-9592836 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-95928362022-10-26 Rhinolophus sinicus virome revealed multiple novel mosquito-borne zoonotic viruses Peng, Chengcheng Zhang, Duo Li, Chenghui Li, Yiquan Zhang, He Li, Nan Xiao, Pengpeng Front Cell Infect Microbiol Cellular and Infection Microbiology To exploit the Rhinolophus sinicus–specific virome, 29 Rhinolophus sinicus were gathered in Lincang, China. Enriched viral sequences of 22 virus families were acquired by metavirome techniques. Hereby, the part of virome in Rhinolophus sinicus, including Chikungunya virus (CHIKV), Getah virus, and Japanese encephalitis virus (JEV) were validated by PCR. Five CHIKV viral sequences were amplified, among which CHIKV-China/B2016C-1 shared the highest homology to CHIKV isolated from Italy in 2007, with the genotype as African ECS. Eight JEV viral sequences were amplified, of which JEV-China/B2016E-1 shared the highest homology with at least 91.3% nt identity with the JEV sequence found in South Korea in 1988 and was classified as genotype III. Notably, JEV was isolated for the first time in Rhinolophus sinicus. The newly isolated JEV-China/B2016-1 could increase infectivity while passaging in Vero cells from BHK-21 cells. Overall, the research sheds insight into the diversity and viral susceptibility dynamics of the virome in Rhinolophus sinicus and reveals new light on the ecology of other important viral hosts. Frontiers Media S.A. 2022-10-11 /pmc/articles/PMC9592836/ /pubmed/36304937 http://dx.doi.org/10.3389/fcimb.2022.960507 Text en Copyright © 2022 Peng, Zhang, Li, Li, Zhang, Li and Xiao https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cellular and Infection Microbiology Peng, Chengcheng Zhang, Duo Li, Chenghui Li, Yiquan Zhang, He Li, Nan Xiao, Pengpeng Rhinolophus sinicus virome revealed multiple novel mosquito-borne zoonotic viruses |
title |
Rhinolophus sinicus virome revealed multiple novel mosquito-borne zoonotic viruses |
title_full |
Rhinolophus sinicus virome revealed multiple novel mosquito-borne zoonotic viruses |
title_fullStr |
Rhinolophus sinicus virome revealed multiple novel mosquito-borne zoonotic viruses |
title_full_unstemmed |
Rhinolophus sinicus virome revealed multiple novel mosquito-borne zoonotic viruses |
title_short |
Rhinolophus sinicus virome revealed multiple novel mosquito-borne zoonotic viruses |
title_sort | rhinolophus sinicus virome revealed multiple novel mosquito-borne zoonotic viruses |
topic | Cellular and Infection Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9592836/ https://www.ncbi.nlm.nih.gov/pubmed/36304937 http://dx.doi.org/10.3389/fcimb.2022.960507 |
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