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Rhinolophus sinicus virome revealed multiple novel mosquito-borne zoonotic viruses

To exploit the Rhinolophus sinicus–specific virome, 29 Rhinolophus sinicus were gathered in Lincang, China. Enriched viral sequences of 22 virus families were acquired by metavirome techniques. Hereby, the part of virome in Rhinolophus sinicus, including Chikungunya virus (CHIKV), Getah virus, and J...

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Autores principales: Peng, Chengcheng, Zhang, Duo, Li, Chenghui, Li, Yiquan, Zhang, He, Li, Nan, Xiao, Pengpeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9592836/
https://www.ncbi.nlm.nih.gov/pubmed/36304937
http://dx.doi.org/10.3389/fcimb.2022.960507
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author Peng, Chengcheng
Zhang, Duo
Li, Chenghui
Li, Yiquan
Zhang, He
Li, Nan
Xiao, Pengpeng
author_facet Peng, Chengcheng
Zhang, Duo
Li, Chenghui
Li, Yiquan
Zhang, He
Li, Nan
Xiao, Pengpeng
author_sort Peng, Chengcheng
collection PubMed
description To exploit the Rhinolophus sinicus–specific virome, 29 Rhinolophus sinicus were gathered in Lincang, China. Enriched viral sequences of 22 virus families were acquired by metavirome techniques. Hereby, the part of virome in Rhinolophus sinicus, including Chikungunya virus (CHIKV), Getah virus, and Japanese encephalitis virus (JEV) were validated by PCR. Five CHIKV viral sequences were amplified, among which CHIKV-China/B2016C-1 shared the highest homology to CHIKV isolated from Italy in 2007, with the genotype as African ECS. Eight JEV viral sequences were amplified, of which JEV-China/B2016E-1 shared the highest homology with at least 91.3% nt identity with the JEV sequence found in South Korea in 1988 and was classified as genotype III. Notably, JEV was isolated for the first time in Rhinolophus sinicus. The newly isolated JEV-China/B2016-1 could increase infectivity while passaging in Vero cells from BHK-21 cells. Overall, the research sheds insight into the diversity and viral susceptibility dynamics of the virome in Rhinolophus sinicus and reveals new light on the ecology of other important viral hosts.
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spelling pubmed-95928362022-10-26 Rhinolophus sinicus virome revealed multiple novel mosquito-borne zoonotic viruses Peng, Chengcheng Zhang, Duo Li, Chenghui Li, Yiquan Zhang, He Li, Nan Xiao, Pengpeng Front Cell Infect Microbiol Cellular and Infection Microbiology To exploit the Rhinolophus sinicus–specific virome, 29 Rhinolophus sinicus were gathered in Lincang, China. Enriched viral sequences of 22 virus families were acquired by metavirome techniques. Hereby, the part of virome in Rhinolophus sinicus, including Chikungunya virus (CHIKV), Getah virus, and Japanese encephalitis virus (JEV) were validated by PCR. Five CHIKV viral sequences were amplified, among which CHIKV-China/B2016C-1 shared the highest homology to CHIKV isolated from Italy in 2007, with the genotype as African ECS. Eight JEV viral sequences were amplified, of which JEV-China/B2016E-1 shared the highest homology with at least 91.3% nt identity with the JEV sequence found in South Korea in 1988 and was classified as genotype III. Notably, JEV was isolated for the first time in Rhinolophus sinicus. The newly isolated JEV-China/B2016-1 could increase infectivity while passaging in Vero cells from BHK-21 cells. Overall, the research sheds insight into the diversity and viral susceptibility dynamics of the virome in Rhinolophus sinicus and reveals new light on the ecology of other important viral hosts. Frontiers Media S.A. 2022-10-11 /pmc/articles/PMC9592836/ /pubmed/36304937 http://dx.doi.org/10.3389/fcimb.2022.960507 Text en Copyright © 2022 Peng, Zhang, Li, Li, Zhang, Li and Xiao https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Peng, Chengcheng
Zhang, Duo
Li, Chenghui
Li, Yiquan
Zhang, He
Li, Nan
Xiao, Pengpeng
Rhinolophus sinicus virome revealed multiple novel mosquito-borne zoonotic viruses
title Rhinolophus sinicus virome revealed multiple novel mosquito-borne zoonotic viruses
title_full Rhinolophus sinicus virome revealed multiple novel mosquito-borne zoonotic viruses
title_fullStr Rhinolophus sinicus virome revealed multiple novel mosquito-borne zoonotic viruses
title_full_unstemmed Rhinolophus sinicus virome revealed multiple novel mosquito-borne zoonotic viruses
title_short Rhinolophus sinicus virome revealed multiple novel mosquito-borne zoonotic viruses
title_sort rhinolophus sinicus virome revealed multiple novel mosquito-borne zoonotic viruses
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9592836/
https://www.ncbi.nlm.nih.gov/pubmed/36304937
http://dx.doi.org/10.3389/fcimb.2022.960507
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