Cargando…
Identification of long non-coding RNA-microRNA-mRNA regulatory modules and their potential roles in drought stress response in wheat (Triticum aestivum L.)
Drought is one of the most severe abiotic stresses that influence wheat production across the globe. Understanding the molecular regulatory network of wheat in response to drought is of great importance in molecular breeding. Noncoding RNAs influence plant development and resistance to abiotic stres...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9592863/ https://www.ncbi.nlm.nih.gov/pubmed/36304395 http://dx.doi.org/10.3389/fpls.2022.1011064 |
_version_ | 1784815025375936512 |
---|---|
author | Li, Ning Liu, Tongtong Guo, Feng Yang, Jinwen Shi, Yugang Wang, Shuguang Sun, Daizhen |
author_facet | Li, Ning Liu, Tongtong Guo, Feng Yang, Jinwen Shi, Yugang Wang, Shuguang Sun, Daizhen |
author_sort | Li, Ning |
collection | PubMed |
description | Drought is one of the most severe abiotic stresses that influence wheat production across the globe. Understanding the molecular regulatory network of wheat in response to drought is of great importance in molecular breeding. Noncoding RNAs influence plant development and resistance to abiotic stresses by regulating gene expression. In this study, whole-transcriptome sequencing was performed on the seedlings of two wheat varieties with contrasting levels of drought tolerance under drought and control conditions to identify long noncoding RNAs (lncRNAs), micro RNAs (miRNAs), and mRNAs related to drought stress and explore the potential lncRNA-miRNA-mRNA regulatory modules in controlling wheat drought stress response. A total of 1515 differentially expressed lncRNAs (DELs), 209 differentially expressed miRNAs (DEMs), and 20462 differentially expressed genes (DEGs) were identified. Of the 20462 DEGs, 1025 were identified as potential wheat drought resistance-related DEGs. Based on the regulatory relationship and expression patterns of DELs, DEMs, and DEGs, 10 DEL-DEM-DEG regulatory modules related to wheat drought stress response were screened, and preliminary expression verification of two important candidate modules was performed. Our results revealed the possible roles of lncRNA-miRNA-mRNA modules in regulatory networks related to drought tolerance and provided useful information as valuable genomic resources in molecular breeding of wheat. |
format | Online Article Text |
id | pubmed-9592863 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-95928632022-10-26 Identification of long non-coding RNA-microRNA-mRNA regulatory modules and their potential roles in drought stress response in wheat (Triticum aestivum L.) Li, Ning Liu, Tongtong Guo, Feng Yang, Jinwen Shi, Yugang Wang, Shuguang Sun, Daizhen Front Plant Sci Plant Science Drought is one of the most severe abiotic stresses that influence wheat production across the globe. Understanding the molecular regulatory network of wheat in response to drought is of great importance in molecular breeding. Noncoding RNAs influence plant development and resistance to abiotic stresses by regulating gene expression. In this study, whole-transcriptome sequencing was performed on the seedlings of two wheat varieties with contrasting levels of drought tolerance under drought and control conditions to identify long noncoding RNAs (lncRNAs), micro RNAs (miRNAs), and mRNAs related to drought stress and explore the potential lncRNA-miRNA-mRNA regulatory modules in controlling wheat drought stress response. A total of 1515 differentially expressed lncRNAs (DELs), 209 differentially expressed miRNAs (DEMs), and 20462 differentially expressed genes (DEGs) were identified. Of the 20462 DEGs, 1025 were identified as potential wheat drought resistance-related DEGs. Based on the regulatory relationship and expression patterns of DELs, DEMs, and DEGs, 10 DEL-DEM-DEG regulatory modules related to wheat drought stress response were screened, and preliminary expression verification of two important candidate modules was performed. Our results revealed the possible roles of lncRNA-miRNA-mRNA modules in regulatory networks related to drought tolerance and provided useful information as valuable genomic resources in molecular breeding of wheat. Frontiers Media S.A. 2022-10-11 /pmc/articles/PMC9592863/ /pubmed/36304395 http://dx.doi.org/10.3389/fpls.2022.1011064 Text en Copyright © 2022 Li, Liu, Guo, Yang, Shi, Wang and Sun https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Li, Ning Liu, Tongtong Guo, Feng Yang, Jinwen Shi, Yugang Wang, Shuguang Sun, Daizhen Identification of long non-coding RNA-microRNA-mRNA regulatory modules and their potential roles in drought stress response in wheat (Triticum aestivum L.) |
title | Identification of long non-coding RNA-microRNA-mRNA regulatory modules and their potential roles in drought stress response in wheat (Triticum aestivum L.) |
title_full | Identification of long non-coding RNA-microRNA-mRNA regulatory modules and their potential roles in drought stress response in wheat (Triticum aestivum L.) |
title_fullStr | Identification of long non-coding RNA-microRNA-mRNA regulatory modules and their potential roles in drought stress response in wheat (Triticum aestivum L.) |
title_full_unstemmed | Identification of long non-coding RNA-microRNA-mRNA regulatory modules and their potential roles in drought stress response in wheat (Triticum aestivum L.) |
title_short | Identification of long non-coding RNA-microRNA-mRNA regulatory modules and their potential roles in drought stress response in wheat (Triticum aestivum L.) |
title_sort | identification of long non-coding rna-microrna-mrna regulatory modules and their potential roles in drought stress response in wheat (triticum aestivum l.) |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9592863/ https://www.ncbi.nlm.nih.gov/pubmed/36304395 http://dx.doi.org/10.3389/fpls.2022.1011064 |
work_keys_str_mv | AT lining identificationoflongnoncodingrnamicrornamrnaregulatorymodulesandtheirpotentialrolesindroughtstressresponseinwheattriticumaestivuml AT liutongtong identificationoflongnoncodingrnamicrornamrnaregulatorymodulesandtheirpotentialrolesindroughtstressresponseinwheattriticumaestivuml AT guofeng identificationoflongnoncodingrnamicrornamrnaregulatorymodulesandtheirpotentialrolesindroughtstressresponseinwheattriticumaestivuml AT yangjinwen identificationoflongnoncodingrnamicrornamrnaregulatorymodulesandtheirpotentialrolesindroughtstressresponseinwheattriticumaestivuml AT shiyugang identificationoflongnoncodingrnamicrornamrnaregulatorymodulesandtheirpotentialrolesindroughtstressresponseinwheattriticumaestivuml AT wangshuguang identificationoflongnoncodingrnamicrornamrnaregulatorymodulesandtheirpotentialrolesindroughtstressresponseinwheattriticumaestivuml AT sundaizhen identificationoflongnoncodingrnamicrornamrnaregulatorymodulesandtheirpotentialrolesindroughtstressresponseinwheattriticumaestivuml |