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β-carotene genetically-enriched lyophilized orange juice increases antioxidant capacity and reduces β-amyloid proteotoxicity and fat accumulation in Caenorhabditis elegans

Citrus sinensis orange juice is an excellent dietary source of β-carotene, a well-known antioxidant. However, β-carotene concentrations are relatively low in most cultivars. We developed a new orange through metabolic engineering strategy (GS) with 33.72-fold increase in β-carotene content compared...

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Autores principales: Raquel Ferreira Paulo, Iolanda, Basílio de Oliveira Caland, Ricardo, Orlando Muñoz Cadavid, Cesar, Martins Melo, Giovanna, Soares De Castro Bezerra, Liliane, Pons, Elsa, Peña, Leandro, de Paula Oliveira, Riva
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9593878/
https://www.ncbi.nlm.nih.gov/pubmed/36304081
http://dx.doi.org/10.1016/j.fochms.2022.100141
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author Raquel Ferreira Paulo, Iolanda
Basílio de Oliveira Caland, Ricardo
Orlando Muñoz Cadavid, Cesar
Martins Melo, Giovanna
Soares De Castro Bezerra, Liliane
Pons, Elsa
Peña, Leandro
de Paula Oliveira, Riva
author_facet Raquel Ferreira Paulo, Iolanda
Basílio de Oliveira Caland, Ricardo
Orlando Muñoz Cadavid, Cesar
Martins Melo, Giovanna
Soares De Castro Bezerra, Liliane
Pons, Elsa
Peña, Leandro
de Paula Oliveira, Riva
author_sort Raquel Ferreira Paulo, Iolanda
collection PubMed
description Citrus sinensis orange juice is an excellent dietary source of β-carotene, a well-known antioxidant. However, β-carotene concentrations are relatively low in most cultivars. We developed a new orange through metabolic engineering strategy (GS) with 33.72-fold increase in β-carotene content compared to its conventional counterpart (CV). Using Caenorhabditis elegans, we found that animals treated with GS showed a greater reduction in intracellular reactive oxygen species (ROS) which is associated with a greater resistance to oxidative stress and induction of the expression of antioxidant genes. Moreover, animals treated with GS orange showed a more effective protection against β-amyloid proteotoxicity and greater hypolipidemic effect under high glucose diet compared to animals treated with CV. These data demonstrate that the increased amount of β-carotene in orange actually provides a greater beneficial effect in C. elegans and a valuable proof of principle to support further studies in mammals and humans.
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spelling pubmed-95938782022-10-26 β-carotene genetically-enriched lyophilized orange juice increases antioxidant capacity and reduces β-amyloid proteotoxicity and fat accumulation in Caenorhabditis elegans Raquel Ferreira Paulo, Iolanda Basílio de Oliveira Caland, Ricardo Orlando Muñoz Cadavid, Cesar Martins Melo, Giovanna Soares De Castro Bezerra, Liliane Pons, Elsa Peña, Leandro de Paula Oliveira, Riva Food Chem (Oxf) Research Article Citrus sinensis orange juice is an excellent dietary source of β-carotene, a well-known antioxidant. However, β-carotene concentrations are relatively low in most cultivars. We developed a new orange through metabolic engineering strategy (GS) with 33.72-fold increase in β-carotene content compared to its conventional counterpart (CV). Using Caenorhabditis elegans, we found that animals treated with GS showed a greater reduction in intracellular reactive oxygen species (ROS) which is associated with a greater resistance to oxidative stress and induction of the expression of antioxidant genes. Moreover, animals treated with GS orange showed a more effective protection against β-amyloid proteotoxicity and greater hypolipidemic effect under high glucose diet compared to animals treated with CV. These data demonstrate that the increased amount of β-carotene in orange actually provides a greater beneficial effect in C. elegans and a valuable proof of principle to support further studies in mammals and humans. Elsevier 2022-10-17 /pmc/articles/PMC9593878/ /pubmed/36304081 http://dx.doi.org/10.1016/j.fochms.2022.100141 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Raquel Ferreira Paulo, Iolanda
Basílio de Oliveira Caland, Ricardo
Orlando Muñoz Cadavid, Cesar
Martins Melo, Giovanna
Soares De Castro Bezerra, Liliane
Pons, Elsa
Peña, Leandro
de Paula Oliveira, Riva
β-carotene genetically-enriched lyophilized orange juice increases antioxidant capacity and reduces β-amyloid proteotoxicity and fat accumulation in Caenorhabditis elegans
title β-carotene genetically-enriched lyophilized orange juice increases antioxidant capacity and reduces β-amyloid proteotoxicity and fat accumulation in Caenorhabditis elegans
title_full β-carotene genetically-enriched lyophilized orange juice increases antioxidant capacity and reduces β-amyloid proteotoxicity and fat accumulation in Caenorhabditis elegans
title_fullStr β-carotene genetically-enriched lyophilized orange juice increases antioxidant capacity and reduces β-amyloid proteotoxicity and fat accumulation in Caenorhabditis elegans
title_full_unstemmed β-carotene genetically-enriched lyophilized orange juice increases antioxidant capacity and reduces β-amyloid proteotoxicity and fat accumulation in Caenorhabditis elegans
title_short β-carotene genetically-enriched lyophilized orange juice increases antioxidant capacity and reduces β-amyloid proteotoxicity and fat accumulation in Caenorhabditis elegans
title_sort β-carotene genetically-enriched lyophilized orange juice increases antioxidant capacity and reduces β-amyloid proteotoxicity and fat accumulation in caenorhabditis elegans
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9593878/
https://www.ncbi.nlm.nih.gov/pubmed/36304081
http://dx.doi.org/10.1016/j.fochms.2022.100141
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