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β-carotene genetically-enriched lyophilized orange juice increases antioxidant capacity and reduces β-amyloid proteotoxicity and fat accumulation in Caenorhabditis elegans
Citrus sinensis orange juice is an excellent dietary source of β-carotene, a well-known antioxidant. However, β-carotene concentrations are relatively low in most cultivars. We developed a new orange through metabolic engineering strategy (GS) with 33.72-fold increase in β-carotene content compared...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9593878/ https://www.ncbi.nlm.nih.gov/pubmed/36304081 http://dx.doi.org/10.1016/j.fochms.2022.100141 |
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author | Raquel Ferreira Paulo, Iolanda Basílio de Oliveira Caland, Ricardo Orlando Muñoz Cadavid, Cesar Martins Melo, Giovanna Soares De Castro Bezerra, Liliane Pons, Elsa Peña, Leandro de Paula Oliveira, Riva |
author_facet | Raquel Ferreira Paulo, Iolanda Basílio de Oliveira Caland, Ricardo Orlando Muñoz Cadavid, Cesar Martins Melo, Giovanna Soares De Castro Bezerra, Liliane Pons, Elsa Peña, Leandro de Paula Oliveira, Riva |
author_sort | Raquel Ferreira Paulo, Iolanda |
collection | PubMed |
description | Citrus sinensis orange juice is an excellent dietary source of β-carotene, a well-known antioxidant. However, β-carotene concentrations are relatively low in most cultivars. We developed a new orange through metabolic engineering strategy (GS) with 33.72-fold increase in β-carotene content compared to its conventional counterpart (CV). Using Caenorhabditis elegans, we found that animals treated with GS showed a greater reduction in intracellular reactive oxygen species (ROS) which is associated with a greater resistance to oxidative stress and induction of the expression of antioxidant genes. Moreover, animals treated with GS orange showed a more effective protection against β-amyloid proteotoxicity and greater hypolipidemic effect under high glucose diet compared to animals treated with CV. These data demonstrate that the increased amount of β-carotene in orange actually provides a greater beneficial effect in C. elegans and a valuable proof of principle to support further studies in mammals and humans. |
format | Online Article Text |
id | pubmed-9593878 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-95938782022-10-26 β-carotene genetically-enriched lyophilized orange juice increases antioxidant capacity and reduces β-amyloid proteotoxicity and fat accumulation in Caenorhabditis elegans Raquel Ferreira Paulo, Iolanda Basílio de Oliveira Caland, Ricardo Orlando Muñoz Cadavid, Cesar Martins Melo, Giovanna Soares De Castro Bezerra, Liliane Pons, Elsa Peña, Leandro de Paula Oliveira, Riva Food Chem (Oxf) Research Article Citrus sinensis orange juice is an excellent dietary source of β-carotene, a well-known antioxidant. However, β-carotene concentrations are relatively low in most cultivars. We developed a new orange through metabolic engineering strategy (GS) with 33.72-fold increase in β-carotene content compared to its conventional counterpart (CV). Using Caenorhabditis elegans, we found that animals treated with GS showed a greater reduction in intracellular reactive oxygen species (ROS) which is associated with a greater resistance to oxidative stress and induction of the expression of antioxidant genes. Moreover, animals treated with GS orange showed a more effective protection against β-amyloid proteotoxicity and greater hypolipidemic effect under high glucose diet compared to animals treated with CV. These data demonstrate that the increased amount of β-carotene in orange actually provides a greater beneficial effect in C. elegans and a valuable proof of principle to support further studies in mammals and humans. Elsevier 2022-10-17 /pmc/articles/PMC9593878/ /pubmed/36304081 http://dx.doi.org/10.1016/j.fochms.2022.100141 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Raquel Ferreira Paulo, Iolanda Basílio de Oliveira Caland, Ricardo Orlando Muñoz Cadavid, Cesar Martins Melo, Giovanna Soares De Castro Bezerra, Liliane Pons, Elsa Peña, Leandro de Paula Oliveira, Riva β-carotene genetically-enriched lyophilized orange juice increases antioxidant capacity and reduces β-amyloid proteotoxicity and fat accumulation in Caenorhabditis elegans |
title | β-carotene genetically-enriched lyophilized orange juice increases antioxidant capacity and reduces β-amyloid proteotoxicity and fat accumulation in Caenorhabditis elegans |
title_full | β-carotene genetically-enriched lyophilized orange juice increases antioxidant capacity and reduces β-amyloid proteotoxicity and fat accumulation in Caenorhabditis elegans |
title_fullStr | β-carotene genetically-enriched lyophilized orange juice increases antioxidant capacity and reduces β-amyloid proteotoxicity and fat accumulation in Caenorhabditis elegans |
title_full_unstemmed | β-carotene genetically-enriched lyophilized orange juice increases antioxidant capacity and reduces β-amyloid proteotoxicity and fat accumulation in Caenorhabditis elegans |
title_short | β-carotene genetically-enriched lyophilized orange juice increases antioxidant capacity and reduces β-amyloid proteotoxicity and fat accumulation in Caenorhabditis elegans |
title_sort | β-carotene genetically-enriched lyophilized orange juice increases antioxidant capacity and reduces β-amyloid proteotoxicity and fat accumulation in caenorhabditis elegans |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9593878/ https://www.ncbi.nlm.nih.gov/pubmed/36304081 http://dx.doi.org/10.1016/j.fochms.2022.100141 |
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