A mAb for the detection of the antiretroviral drug emtricitabine

Antibody-based testing for emtricitabine (FTC), a critical component of pre-exposure prophylaxis and antiretroviral therapy, would provide low-cost detection for clinical monitoring to improve adherence. We developed a mAb (5D2) to FTC and demonstrated its high specificity and physiologically releva...

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Autores principales: Youngpairoj, Ae S., Vanderford, Thomas H., Reed, Matthew S., Granade, Timothy C., Pau, Chou-Pong, Pohl, Jan, Switzer, William M., Heneine, Walid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Lippincott Williams & Wilkins 2022
Materias:
Research Letters
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9594139/
https://www.ncbi.nlm.nih.gov/pubmed/36111696
http://dx.doi.org/10.1097/QAD.0000000000003357
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author Youngpairoj, Ae S.
Vanderford, Thomas H.
Reed, Matthew S.
Granade, Timothy C.
Pau, Chou-Pong
Pohl, Jan
Switzer, William M.
Heneine, Walid
author_facet Youngpairoj, Ae S.
Vanderford, Thomas H.
Reed, Matthew S.
Granade, Timothy C.
Pau, Chou-Pong
Pohl, Jan
Switzer, William M.
Heneine, Walid
author_sort Youngpairoj, Ae S.
collection PubMed
description Antibody-based testing for emtricitabine (FTC), a critical component of pre-exposure prophylaxis and antiretroviral therapy, would provide low-cost detection for clinical monitoring to improve adherence. We developed a mAb (5D2) to FTC and demonstrated its high specificity and physiologically relevant linear range of detection in a competitive enzyme immunoassay. Thus, this mAb is a key reagent that will enable simple and low-cost lateral flow assays and enzyme immunoassays for adherence monitoring.
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spelling pubmed-95941392022-10-27 A mAb for the detection of the antiretroviral drug emtricitabine Youngpairoj, Ae S. Vanderford, Thomas H. Reed, Matthew S. Granade, Timothy C. Pau, Chou-Pong Pohl, Jan Switzer, William M. Heneine, Walid AIDS Research Letters Antibody-based testing for emtricitabine (FTC), a critical component of pre-exposure prophylaxis and antiretroviral therapy, would provide low-cost detection for clinical monitoring to improve adherence. We developed a mAb (5D2) to FTC and demonstrated its high specificity and physiologically relevant linear range of detection in a competitive enzyme immunoassay. Thus, this mAb is a key reagent that will enable simple and low-cost lateral flow assays and enzyme immunoassays for adherence monitoring. Lippincott Williams & Wilkins 2022-11-01 2022-09-16 /pmc/articles/PMC9594139/ /pubmed/36111696 http://dx.doi.org/10.1097/QAD.0000000000003357 Text en Copyright © 2022 The Author(s). Published by Wolters Kluwer Health, Inc. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. http://creativecommons.org/licenses/by-nc-nd/4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Research Letters
Youngpairoj, Ae S.
Vanderford, Thomas H.
Reed, Matthew S.
Granade, Timothy C.
Pau, Chou-Pong
Pohl, Jan
Switzer, William M.
Heneine, Walid
A mAb for the detection of the antiretroviral drug emtricitabine
title A mAb for the detection of the antiretroviral drug emtricitabine
title_full A mAb for the detection of the antiretroviral drug emtricitabine
title_fullStr A mAb for the detection of the antiretroviral drug emtricitabine
title_full_unstemmed A mAb for the detection of the antiretroviral drug emtricitabine
title_short A mAb for the detection of the antiretroviral drug emtricitabine
title_sort mab for the detection of the antiretroviral drug emtricitabine
topic Research Letters
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9594139/
https://www.ncbi.nlm.nih.gov/pubmed/36111696
http://dx.doi.org/10.1097/QAD.0000000000003357
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