A Versatile in Vivo DNA Assembly Toolbox for Fungal Strain Engineering

[Image: see text] Efficient homologous recombination in baker’s yeast allows accurate fusion of DNA fragments via short identical sequence tags in vivo. Eliminating the need for an Escherichia coli cloning step speeds up genetic engineering of this yeast and sets the stage for large high-throughput...

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Detalles Bibliográficos
Autores principales: Jarczynska, Zofia Dorota, Garcia Vanegas, Katherina, Deichmann, Marcus, Nørskov Jensen, Christina, Scheeper, Marouschka Jasmijn, Futyma, Malgorzata Ewa, Strucko, Tomas, Jares Contesini, Fabiano, Sparholt Jørgensen, Tue, Blæsbjerg Hoof, Jakob, Hasbro Mortensen, Uffe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9594312/
https://www.ncbi.nlm.nih.gov/pubmed/36126183
http://dx.doi.org/10.1021/acssynbio.2c00159

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