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A p-Coumaroyl-CoA Biosensor for Dynamic Regulation of Naringenin Biosynthesis in Saccharomyces cerevisiae

[Image: see text] In vivo biosensors that can convert metabolite concentrations into measurable output signals are valuable tools for high-throughput screening and dynamic pathway control in the field of metabolic engineering. Here, we present a novel biosensor in Saccharomyces cerevisiae that is re...

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Autores principales: Liu, Dany, Sica, Maria Sole, Mao, Jiwei, Chao, Lucy Fang-I, Siewers, Verena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9594313/
https://www.ncbi.nlm.nih.gov/pubmed/36137537
http://dx.doi.org/10.1021/acssynbio.2c00111
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author Liu, Dany
Sica, Maria Sole
Mao, Jiwei
Chao, Lucy Fang-I
Siewers, Verena
author_facet Liu, Dany
Sica, Maria Sole
Mao, Jiwei
Chao, Lucy Fang-I
Siewers, Verena
author_sort Liu, Dany
collection PubMed
description [Image: see text] In vivo biosensors that can convert metabolite concentrations into measurable output signals are valuable tools for high-throughput screening and dynamic pathway control in the field of metabolic engineering. Here, we present a novel biosensor in Saccharomyces cerevisiae that is responsive to p-coumaroyl-CoA, a central precursor of many flavonoids. The sensor is based on the transcriptional repressor CouR from Rhodopseudomonas palustris and was applied in combination with a previously developed malonyl-CoA biosensor for dual regulation of p-coumaroyl-CoA synthesis within the naringenin production pathway. Using this approach, we obtained a naringenin titer of 47.3 mg/L upon external precursor feeding, representing a 15-fold increase over the nonregulated system.
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spelling pubmed-95943132022-10-26 A p-Coumaroyl-CoA Biosensor for Dynamic Regulation of Naringenin Biosynthesis in Saccharomyces cerevisiae Liu, Dany Sica, Maria Sole Mao, Jiwei Chao, Lucy Fang-I Siewers, Verena ACS Synth Biol [Image: see text] In vivo biosensors that can convert metabolite concentrations into measurable output signals are valuable tools for high-throughput screening and dynamic pathway control in the field of metabolic engineering. Here, we present a novel biosensor in Saccharomyces cerevisiae that is responsive to p-coumaroyl-CoA, a central precursor of many flavonoids. The sensor is based on the transcriptional repressor CouR from Rhodopseudomonas palustris and was applied in combination with a previously developed malonyl-CoA biosensor for dual regulation of p-coumaroyl-CoA synthesis within the naringenin production pathway. Using this approach, we obtained a naringenin titer of 47.3 mg/L upon external precursor feeding, representing a 15-fold increase over the nonregulated system. American Chemical Society 2022-09-22 2022-10-21 /pmc/articles/PMC9594313/ /pubmed/36137537 http://dx.doi.org/10.1021/acssynbio.2c00111 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Liu, Dany
Sica, Maria Sole
Mao, Jiwei
Chao, Lucy Fang-I
Siewers, Verena
A p-Coumaroyl-CoA Biosensor for Dynamic Regulation of Naringenin Biosynthesis in Saccharomyces cerevisiae
title A p-Coumaroyl-CoA Biosensor for Dynamic Regulation of Naringenin Biosynthesis in Saccharomyces cerevisiae
title_full A p-Coumaroyl-CoA Biosensor for Dynamic Regulation of Naringenin Biosynthesis in Saccharomyces cerevisiae
title_fullStr A p-Coumaroyl-CoA Biosensor for Dynamic Regulation of Naringenin Biosynthesis in Saccharomyces cerevisiae
title_full_unstemmed A p-Coumaroyl-CoA Biosensor for Dynamic Regulation of Naringenin Biosynthesis in Saccharomyces cerevisiae
title_short A p-Coumaroyl-CoA Biosensor for Dynamic Regulation of Naringenin Biosynthesis in Saccharomyces cerevisiae
title_sort p-coumaroyl-coa biosensor for dynamic regulation of naringenin biosynthesis in saccharomyces cerevisiae
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9594313/
https://www.ncbi.nlm.nih.gov/pubmed/36137537
http://dx.doi.org/10.1021/acssynbio.2c00111
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