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Synonymous Codons and Hydrophobicity Optimization of Post-translational Signal Peptide PelB Increase Phage Display Efficiency of DARPins
[Image: see text] DsbA leader peptide targets proteins for cotranslational translocation by signal recognition particle (SRP) pathway and has been the standard signal sequence for filamentous phage display of fast-folding Designed Ankyrin Repeat Proteins (DARPins). In contrast, translocation of DARP...
Autores principales: | Kulmala, Antti, Lappalainen, Matias, Lamminmäki, Urpo, Huovinen, Tuomas |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9594773/ https://www.ncbi.nlm.nih.gov/pubmed/36178799 http://dx.doi.org/10.1021/acssynbio.2c00260 |
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