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Optogenetic Control of Bacterial Expression by Red Light

[Image: see text] In optogenetics, as in nature, sensory photoreceptors serve to control cellular processes by light. Bacteriophytochrome (BphP) photoreceptors sense red and far-red light via a biliverdin chromophore and, in response, cycle between the spectroscopically, structurally, and functional...

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Autores principales: Multamäki, Elina, García de Fuentes, Andrés, Sieryi, Oleksii, Bykov, Alexander, Gerken, Uwe, Ranzani, Américo Tavares, Köhler, Jürgen, Meglinski, Igor, Möglich, Andreas, Takala, Heikki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9594775/
https://www.ncbi.nlm.nih.gov/pubmed/35998606
http://dx.doi.org/10.1021/acssynbio.2c00259
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author Multamäki, Elina
García de Fuentes, Andrés
Sieryi, Oleksii
Bykov, Alexander
Gerken, Uwe
Ranzani, Américo Tavares
Köhler, Jürgen
Meglinski, Igor
Möglich, Andreas
Takala, Heikki
author_facet Multamäki, Elina
García de Fuentes, Andrés
Sieryi, Oleksii
Bykov, Alexander
Gerken, Uwe
Ranzani, Américo Tavares
Köhler, Jürgen
Meglinski, Igor
Möglich, Andreas
Takala, Heikki
author_sort Multamäki, Elina
collection PubMed
description [Image: see text] In optogenetics, as in nature, sensory photoreceptors serve to control cellular processes by light. Bacteriophytochrome (BphP) photoreceptors sense red and far-red light via a biliverdin chromophore and, in response, cycle between the spectroscopically, structurally, and functionally distinct Pr and Pfr states. BphPs commonly belong to two-component systems that control the phosphorylation of cognate response regulators and downstream gene expression through histidine kinase modules. We recently demonstrated that the paradigm BphP from Deinococcus radiodurans exclusively acts as a phosphatase but that its photosensory module can control the histidine kinase activity of homologous receptors. Here, we apply this insight to reprogram two widely used setups for bacterial gene expression from blue-light to red-light control. The resultant pREDusk and pREDawn systems allow gene expression to be regulated down and up, respectively, uniformly under red light by 100-fold or more. Both setups are realized as portable, single plasmids that encode all necessary components including the biliverdin-producing machinery. The triggering by red light affords high spatial resolution down to the single-cell level. As pREDusk and pREDawn respond sensitively to red light, they support multiplexing with optogenetic systems sensitive to other light colors. Owing to the superior tissue penetration of red light, the pREDawn system can be triggered at therapeutically safe light intensities through material layers, replicating the optical properties of the skin and skull. Given these advantages, pREDusk and pREDawn enable red-light-regulated expression for diverse use cases in bacteria.
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spelling pubmed-95947752022-10-26 Optogenetic Control of Bacterial Expression by Red Light Multamäki, Elina García de Fuentes, Andrés Sieryi, Oleksii Bykov, Alexander Gerken, Uwe Ranzani, Américo Tavares Köhler, Jürgen Meglinski, Igor Möglich, Andreas Takala, Heikki ACS Synth Biol [Image: see text] In optogenetics, as in nature, sensory photoreceptors serve to control cellular processes by light. Bacteriophytochrome (BphP) photoreceptors sense red and far-red light via a biliverdin chromophore and, in response, cycle between the spectroscopically, structurally, and functionally distinct Pr and Pfr states. BphPs commonly belong to two-component systems that control the phosphorylation of cognate response regulators and downstream gene expression through histidine kinase modules. We recently demonstrated that the paradigm BphP from Deinococcus radiodurans exclusively acts as a phosphatase but that its photosensory module can control the histidine kinase activity of homologous receptors. Here, we apply this insight to reprogram two widely used setups for bacterial gene expression from blue-light to red-light control. The resultant pREDusk and pREDawn systems allow gene expression to be regulated down and up, respectively, uniformly under red light by 100-fold or more. Both setups are realized as portable, single plasmids that encode all necessary components including the biliverdin-producing machinery. The triggering by red light affords high spatial resolution down to the single-cell level. As pREDusk and pREDawn respond sensitively to red light, they support multiplexing with optogenetic systems sensitive to other light colors. Owing to the superior tissue penetration of red light, the pREDawn system can be triggered at therapeutically safe light intensities through material layers, replicating the optical properties of the skin and skull. Given these advantages, pREDusk and pREDawn enable red-light-regulated expression for diverse use cases in bacteria. American Chemical Society 2022-08-23 2022-10-21 /pmc/articles/PMC9594775/ /pubmed/35998606 http://dx.doi.org/10.1021/acssynbio.2c00259 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Multamäki, Elina
García de Fuentes, Andrés
Sieryi, Oleksii
Bykov, Alexander
Gerken, Uwe
Ranzani, Américo Tavares
Köhler, Jürgen
Meglinski, Igor
Möglich, Andreas
Takala, Heikki
Optogenetic Control of Bacterial Expression by Red Light
title Optogenetic Control of Bacterial Expression by Red Light
title_full Optogenetic Control of Bacterial Expression by Red Light
title_fullStr Optogenetic Control of Bacterial Expression by Red Light
title_full_unstemmed Optogenetic Control of Bacterial Expression by Red Light
title_short Optogenetic Control of Bacterial Expression by Red Light
title_sort optogenetic control of bacterial expression by red light
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9594775/
https://www.ncbi.nlm.nih.gov/pubmed/35998606
http://dx.doi.org/10.1021/acssynbio.2c00259
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