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Optogenetic Control of Bacterial Expression by Red Light
[Image: see text] In optogenetics, as in nature, sensory photoreceptors serve to control cellular processes by light. Bacteriophytochrome (BphP) photoreceptors sense red and far-red light via a biliverdin chromophore and, in response, cycle between the spectroscopically, structurally, and functional...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9594775/ https://www.ncbi.nlm.nih.gov/pubmed/35998606 http://dx.doi.org/10.1021/acssynbio.2c00259 |
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author | Multamäki, Elina García de Fuentes, Andrés Sieryi, Oleksii Bykov, Alexander Gerken, Uwe Ranzani, Américo Tavares Köhler, Jürgen Meglinski, Igor Möglich, Andreas Takala, Heikki |
author_facet | Multamäki, Elina García de Fuentes, Andrés Sieryi, Oleksii Bykov, Alexander Gerken, Uwe Ranzani, Américo Tavares Köhler, Jürgen Meglinski, Igor Möglich, Andreas Takala, Heikki |
author_sort | Multamäki, Elina |
collection | PubMed |
description | [Image: see text] In optogenetics, as in nature, sensory photoreceptors serve to control cellular processes by light. Bacteriophytochrome (BphP) photoreceptors sense red and far-red light via a biliverdin chromophore and, in response, cycle between the spectroscopically, structurally, and functionally distinct Pr and Pfr states. BphPs commonly belong to two-component systems that control the phosphorylation of cognate response regulators and downstream gene expression through histidine kinase modules. We recently demonstrated that the paradigm BphP from Deinococcus radiodurans exclusively acts as a phosphatase but that its photosensory module can control the histidine kinase activity of homologous receptors. Here, we apply this insight to reprogram two widely used setups for bacterial gene expression from blue-light to red-light control. The resultant pREDusk and pREDawn systems allow gene expression to be regulated down and up, respectively, uniformly under red light by 100-fold or more. Both setups are realized as portable, single plasmids that encode all necessary components including the biliverdin-producing machinery. The triggering by red light affords high spatial resolution down to the single-cell level. As pREDusk and pREDawn respond sensitively to red light, they support multiplexing with optogenetic systems sensitive to other light colors. Owing to the superior tissue penetration of red light, the pREDawn system can be triggered at therapeutically safe light intensities through material layers, replicating the optical properties of the skin and skull. Given these advantages, pREDusk and pREDawn enable red-light-regulated expression for diverse use cases in bacteria. |
format | Online Article Text |
id | pubmed-9594775 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-95947752022-10-26 Optogenetic Control of Bacterial Expression by Red Light Multamäki, Elina García de Fuentes, Andrés Sieryi, Oleksii Bykov, Alexander Gerken, Uwe Ranzani, Américo Tavares Köhler, Jürgen Meglinski, Igor Möglich, Andreas Takala, Heikki ACS Synth Biol [Image: see text] In optogenetics, as in nature, sensory photoreceptors serve to control cellular processes by light. Bacteriophytochrome (BphP) photoreceptors sense red and far-red light via a biliverdin chromophore and, in response, cycle between the spectroscopically, structurally, and functionally distinct Pr and Pfr states. BphPs commonly belong to two-component systems that control the phosphorylation of cognate response regulators and downstream gene expression through histidine kinase modules. We recently demonstrated that the paradigm BphP from Deinococcus radiodurans exclusively acts as a phosphatase but that its photosensory module can control the histidine kinase activity of homologous receptors. Here, we apply this insight to reprogram two widely used setups for bacterial gene expression from blue-light to red-light control. The resultant pREDusk and pREDawn systems allow gene expression to be regulated down and up, respectively, uniformly under red light by 100-fold or more. Both setups are realized as portable, single plasmids that encode all necessary components including the biliverdin-producing machinery. The triggering by red light affords high spatial resolution down to the single-cell level. As pREDusk and pREDawn respond sensitively to red light, they support multiplexing with optogenetic systems sensitive to other light colors. Owing to the superior tissue penetration of red light, the pREDawn system can be triggered at therapeutically safe light intensities through material layers, replicating the optical properties of the skin and skull. Given these advantages, pREDusk and pREDawn enable red-light-regulated expression for diverse use cases in bacteria. American Chemical Society 2022-08-23 2022-10-21 /pmc/articles/PMC9594775/ /pubmed/35998606 http://dx.doi.org/10.1021/acssynbio.2c00259 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Multamäki, Elina García de Fuentes, Andrés Sieryi, Oleksii Bykov, Alexander Gerken, Uwe Ranzani, Américo Tavares Köhler, Jürgen Meglinski, Igor Möglich, Andreas Takala, Heikki Optogenetic Control of Bacterial Expression by Red Light |
title | Optogenetic Control
of Bacterial Expression by Red
Light |
title_full | Optogenetic Control
of Bacterial Expression by Red
Light |
title_fullStr | Optogenetic Control
of Bacterial Expression by Red
Light |
title_full_unstemmed | Optogenetic Control
of Bacterial Expression by Red
Light |
title_short | Optogenetic Control
of Bacterial Expression by Red
Light |
title_sort | optogenetic control
of bacterial expression by red
light |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9594775/ https://www.ncbi.nlm.nih.gov/pubmed/35998606 http://dx.doi.org/10.1021/acssynbio.2c00259 |
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