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The Relationship Between Klotho and SIRT1 Expression in Renal Aging Related Disease

BACKGROUND: This study focused on renal arteriosclerosis and aimed to explore the relationship between Klotho and SIRT1 by morphological staining, which will help to provide new ideas for the treatment of renal-aging-related diseases and a theoretical basis for the development of new drugs. METHODS:...

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Autores principales: Su, Hong, Gao, Diansa, Chen, Yanlin, Zuo, Zhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9595124/
https://www.ncbi.nlm.nih.gov/pubmed/36304672
http://dx.doi.org/10.2147/IJGM.S384119
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author Su, Hong
Gao, Diansa
Chen, Yanlin
Zuo, Zhong
author_facet Su, Hong
Gao, Diansa
Chen, Yanlin
Zuo, Zhong
author_sort Su, Hong
collection PubMed
description BACKGROUND: This study focused on renal arteriosclerosis and aimed to explore the relationship between Klotho and SIRT1 by morphological staining, which will help to provide new ideas for the treatment of renal-aging-related diseases and a theoretical basis for the development of new drugs. METHODS: Kidney tissue samples were collected from patients who underwent nephrectomy. HK-2 cells were cultured. The Hematoxylin-eosin (HE) staining, Periodic Acid-Schiff (PAS) staining, Masson’s Trichrome staining, Immunohistochemistry (IHC) staining, Immunofluorescence (ICC) and bioinformatics means were used for this study. RESULTS: HE staining showed that glomerulosclerosis was atrophic and cast was significantly increased luminal narrowing of renal arterioles in aging group. PAS staining showed that the number of podocytes was reduced, the mesangial matrix expansion and the intimal fibrosis of renal arterioles. Masson’s trichrome staining showed that there was massive collagen proliferation in the tubulointerstitial in aging group, as well as intimal thickening and fibrin deposition in the tubular walls of arterioles. IHC staining showed that the expression of Klotho and SIRT1 protein was downregulated in aging group and the trend of the two was positively correlated (P < 0.01). Klotho and SIRT1 co-localized in HK-2 cells and kidney tissue. The GEPIA database analysis showed a significant positive correlation between Klotho and SIRT1 in multiple human tissues and tumors. CONCLUSION: Glomerulosclerosis in aging group is accompanied by low expression of Klotho and SIRT1 in renal tissue, and Klotho is positively correlated with SIRT1. Klotho-SIRT1 pathway may be involved in the occurrence and development of renal-aging-related diseases.
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spelling pubmed-95951242022-10-26 The Relationship Between Klotho and SIRT1 Expression in Renal Aging Related Disease Su, Hong Gao, Diansa Chen, Yanlin Zuo, Zhong Int J Gen Med Original Research BACKGROUND: This study focused on renal arteriosclerosis and aimed to explore the relationship between Klotho and SIRT1 by morphological staining, which will help to provide new ideas for the treatment of renal-aging-related diseases and a theoretical basis for the development of new drugs. METHODS: Kidney tissue samples were collected from patients who underwent nephrectomy. HK-2 cells were cultured. The Hematoxylin-eosin (HE) staining, Periodic Acid-Schiff (PAS) staining, Masson’s Trichrome staining, Immunohistochemistry (IHC) staining, Immunofluorescence (ICC) and bioinformatics means were used for this study. RESULTS: HE staining showed that glomerulosclerosis was atrophic and cast was significantly increased luminal narrowing of renal arterioles in aging group. PAS staining showed that the number of podocytes was reduced, the mesangial matrix expansion and the intimal fibrosis of renal arterioles. Masson’s trichrome staining showed that there was massive collagen proliferation in the tubulointerstitial in aging group, as well as intimal thickening and fibrin deposition in the tubular walls of arterioles. IHC staining showed that the expression of Klotho and SIRT1 protein was downregulated in aging group and the trend of the two was positively correlated (P < 0.01). Klotho and SIRT1 co-localized in HK-2 cells and kidney tissue. The GEPIA database analysis showed a significant positive correlation between Klotho and SIRT1 in multiple human tissues and tumors. CONCLUSION: Glomerulosclerosis in aging group is accompanied by low expression of Klotho and SIRT1 in renal tissue, and Klotho is positively correlated with SIRT1. Klotho-SIRT1 pathway may be involved in the occurrence and development of renal-aging-related diseases. Dove 2022-10-21 /pmc/articles/PMC9595124/ /pubmed/36304672 http://dx.doi.org/10.2147/IJGM.S384119 Text en © 2022 Su et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Su, Hong
Gao, Diansa
Chen, Yanlin
Zuo, Zhong
The Relationship Between Klotho and SIRT1 Expression in Renal Aging Related Disease
title The Relationship Between Klotho and SIRT1 Expression in Renal Aging Related Disease
title_full The Relationship Between Klotho and SIRT1 Expression in Renal Aging Related Disease
title_fullStr The Relationship Between Klotho and SIRT1 Expression in Renal Aging Related Disease
title_full_unstemmed The Relationship Between Klotho and SIRT1 Expression in Renal Aging Related Disease
title_short The Relationship Between Klotho and SIRT1 Expression in Renal Aging Related Disease
title_sort relationship between klotho and sirt1 expression in renal aging related disease
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9595124/
https://www.ncbi.nlm.nih.gov/pubmed/36304672
http://dx.doi.org/10.2147/IJGM.S384119
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