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Delta-catenin attenuates medulloblastoma cell invasion by targeting EMT pathway

Background: Medulloblastoma is the most common pediatric malignant tumor in central nervous system. Although its prognosis has been improved enormously by the combination treatments with surgery, radiotherapy, and chemotherapy, it still could progress via invasion and distant dissemination. We aimed...

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Autores principales: Hu, Yuanjun, Zhu, Sihan, Xu, Rizhen, Wang, Manxia, Chen, Furong, Zhang, Zeshun, Feng, Binghong, Wang, Jian, Chen, Zhongping, Wang, Jing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9595215/
https://www.ncbi.nlm.nih.gov/pubmed/36303547
http://dx.doi.org/10.3389/fgene.2022.867872
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author Hu, Yuanjun
Zhu, Sihan
Xu, Rizhen
Wang, Manxia
Chen, Furong
Zhang, Zeshun
Feng, Binghong
Wang, Jian
Chen, Zhongping
Wang, Jing
author_facet Hu, Yuanjun
Zhu, Sihan
Xu, Rizhen
Wang, Manxia
Chen, Furong
Zhang, Zeshun
Feng, Binghong
Wang, Jian
Chen, Zhongping
Wang, Jing
author_sort Hu, Yuanjun
collection PubMed
description Background: Medulloblastoma is the most common pediatric malignant tumor in central nervous system. Although its prognosis has been improved enormously by the combination treatments with surgery, radiotherapy, and chemotherapy, it still could progress via invasion and distant dissemination. We aimed to investigate molecular mechanisms of medulloblastoma invasion in the current work. Methods: The gene expression profile of medulloblastoma were analyzed based on the data deposited in Gene Expression Omnibus (GEO) and filtered according to brain specific proteins in the Uniprot. Delta-catenin was identified and further analyzed about its expression and roles in the prognosis of medulloblastoma patient. The function of delta-catenin on cell invasion and migration were investigated by transwell and wound healing assay. Whether delta-catenin participates in the epithelial-mesenchymal transition (EMT) regulated invasion was also studied. Results: Delta-catenin expression was highly upregulated in tumor tissues compared to normal tissues from medulloblastoma patients in five independent, nonoverlapping cohorts. Furthermore, delta-catenin expression level was upregulated in WNT subgroup, and significantly correlated with better prognosis, and associated with metastasis through GEO database analysis. Functional assays indicated that delta-catenin inhibited medulloblastoma cell invasion and migration through regulating the key factors of EMT pathway, such as E-cadherin and vimentin. Conclusion: Delta-catenin might be a positive predictor for prognosis of medulloblastoma patients, through attenuating medulloblastoma cell invasion by inhibiting EMT pathway.
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spelling pubmed-95952152022-10-26 Delta-catenin attenuates medulloblastoma cell invasion by targeting EMT pathway Hu, Yuanjun Zhu, Sihan Xu, Rizhen Wang, Manxia Chen, Furong Zhang, Zeshun Feng, Binghong Wang, Jian Chen, Zhongping Wang, Jing Front Genet Genetics Background: Medulloblastoma is the most common pediatric malignant tumor in central nervous system. Although its prognosis has been improved enormously by the combination treatments with surgery, radiotherapy, and chemotherapy, it still could progress via invasion and distant dissemination. We aimed to investigate molecular mechanisms of medulloblastoma invasion in the current work. Methods: The gene expression profile of medulloblastoma were analyzed based on the data deposited in Gene Expression Omnibus (GEO) and filtered according to brain specific proteins in the Uniprot. Delta-catenin was identified and further analyzed about its expression and roles in the prognosis of medulloblastoma patient. The function of delta-catenin on cell invasion and migration were investigated by transwell and wound healing assay. Whether delta-catenin participates in the epithelial-mesenchymal transition (EMT) regulated invasion was also studied. Results: Delta-catenin expression was highly upregulated in tumor tissues compared to normal tissues from medulloblastoma patients in five independent, nonoverlapping cohorts. Furthermore, delta-catenin expression level was upregulated in WNT subgroup, and significantly correlated with better prognosis, and associated with metastasis through GEO database analysis. Functional assays indicated that delta-catenin inhibited medulloblastoma cell invasion and migration through regulating the key factors of EMT pathway, such as E-cadherin and vimentin. Conclusion: Delta-catenin might be a positive predictor for prognosis of medulloblastoma patients, through attenuating medulloblastoma cell invasion by inhibiting EMT pathway. Frontiers Media S.A. 2022-10-11 /pmc/articles/PMC9595215/ /pubmed/36303547 http://dx.doi.org/10.3389/fgene.2022.867872 Text en Copyright © 2022 Hu, Zhu, Xu, Wang, Chen, Zhang, Feng, Wang, Chen and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Hu, Yuanjun
Zhu, Sihan
Xu, Rizhen
Wang, Manxia
Chen, Furong
Zhang, Zeshun
Feng, Binghong
Wang, Jian
Chen, Zhongping
Wang, Jing
Delta-catenin attenuates medulloblastoma cell invasion by targeting EMT pathway
title Delta-catenin attenuates medulloblastoma cell invasion by targeting EMT pathway
title_full Delta-catenin attenuates medulloblastoma cell invasion by targeting EMT pathway
title_fullStr Delta-catenin attenuates medulloblastoma cell invasion by targeting EMT pathway
title_full_unstemmed Delta-catenin attenuates medulloblastoma cell invasion by targeting EMT pathway
title_short Delta-catenin attenuates medulloblastoma cell invasion by targeting EMT pathway
title_sort delta-catenin attenuates medulloblastoma cell invasion by targeting emt pathway
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9595215/
https://www.ncbi.nlm.nih.gov/pubmed/36303547
http://dx.doi.org/10.3389/fgene.2022.867872
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