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cNap1 bridges centriole contact sites to maintain centrosome cohesion

Centrioles are non-membrane-bound organelles that participate in fundamental cellular processes through their ability to form physical contacts with other structures. During interphase, two mature centrioles can associate to form a single centrosome—a phenomenon known as centrosome cohesion. Centros...

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Autor principal: Mahen, Robert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9595518/
https://www.ncbi.nlm.nih.gov/pubmed/36282799
http://dx.doi.org/10.1371/journal.pbio.3001854
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author Mahen, Robert
author_facet Mahen, Robert
author_sort Mahen, Robert
collection PubMed
description Centrioles are non-membrane-bound organelles that participate in fundamental cellular processes through their ability to form physical contacts with other structures. During interphase, two mature centrioles can associate to form a single centrosome—a phenomenon known as centrosome cohesion. Centrosome cohesion is important for processes such as cell migration, and yet how it is maintained is unclear. Current models indicate that pericentriolar fibres termed rootlets, also known as the centrosome linker, entangle to maintain centriole proximity. Here, I uncover a centriole–centriole contact site and mechanism of centrosome cohesion based on coalescence of the proximal centriole component cNap1. Using live-cell imaging of endogenously tagged cNap1, I show that proximal centrioles form dynamic contacts in response to physical force from the cytoskeleton. Expansion microscopy reveals that cNap1 bridges between these contact sites, physically linking proximal centrioles on the nanoscale. Fluorescence correlation spectroscopy (FCS)-calibrated imaging shows that cNap1 accumulates at nearly micromolar concentrations on proximal centrioles, corresponding to a few hundred protein copy numbers. When ectopically tethered to organelles such as lysosomes, cNap1 forms viscous and cohesive assemblies that promote organelle spatial proximity. These results suggest a mechanism of centrosome cohesion by cNap1 at the proximal centriole and illustrate how a non-membrane-bound organelle forms organelle contact sites.
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spelling pubmed-95955182022-10-26 cNap1 bridges centriole contact sites to maintain centrosome cohesion Mahen, Robert PLoS Biol Update Article Centrioles are non-membrane-bound organelles that participate in fundamental cellular processes through their ability to form physical contacts with other structures. During interphase, two mature centrioles can associate to form a single centrosome—a phenomenon known as centrosome cohesion. Centrosome cohesion is important for processes such as cell migration, and yet how it is maintained is unclear. Current models indicate that pericentriolar fibres termed rootlets, also known as the centrosome linker, entangle to maintain centriole proximity. Here, I uncover a centriole–centriole contact site and mechanism of centrosome cohesion based on coalescence of the proximal centriole component cNap1. Using live-cell imaging of endogenously tagged cNap1, I show that proximal centrioles form dynamic contacts in response to physical force from the cytoskeleton. Expansion microscopy reveals that cNap1 bridges between these contact sites, physically linking proximal centrioles on the nanoscale. Fluorescence correlation spectroscopy (FCS)-calibrated imaging shows that cNap1 accumulates at nearly micromolar concentrations on proximal centrioles, corresponding to a few hundred protein copy numbers. When ectopically tethered to organelles such as lysosomes, cNap1 forms viscous and cohesive assemblies that promote organelle spatial proximity. These results suggest a mechanism of centrosome cohesion by cNap1 at the proximal centriole and illustrate how a non-membrane-bound organelle forms organelle contact sites. Public Library of Science 2022-10-25 /pmc/articles/PMC9595518/ /pubmed/36282799 http://dx.doi.org/10.1371/journal.pbio.3001854 Text en © 2022 Robert Mahen https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Update Article
Mahen, Robert
cNap1 bridges centriole contact sites to maintain centrosome cohesion
title cNap1 bridges centriole contact sites to maintain centrosome cohesion
title_full cNap1 bridges centriole contact sites to maintain centrosome cohesion
title_fullStr cNap1 bridges centriole contact sites to maintain centrosome cohesion
title_full_unstemmed cNap1 bridges centriole contact sites to maintain centrosome cohesion
title_short cNap1 bridges centriole contact sites to maintain centrosome cohesion
title_sort cnap1 bridges centriole contact sites to maintain centrosome cohesion
topic Update Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9595518/
https://www.ncbi.nlm.nih.gov/pubmed/36282799
http://dx.doi.org/10.1371/journal.pbio.3001854
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