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Antibacterial activity of peptaibols from Trichoderma longibrachiatum SMF2 against gram-negative Xanthomonas oryzae pv. oryzae, the causal agent of bacterial leaf blight on rice

Bacterial leaf blight caused by Gram-negative pathogen Xanthomonas oryzae pv. oryzae (Xoo) is one of the most destructive bacterial diseases on rice. Due to the resistance, toxicity and environmental issues of chemical bactericides, new biological strategies are still in need. Although peptaibols pr...

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Autores principales: Zhang, Yu-Qiang, Zhang, Shan, Sun, Mei-Ling, Su, Hai-Nan, Li, Hao-Yang, Kun-Liu, Zhang, Yu-Zhong, Chen, Xiu-Lan, Cao, Hai-Yan, Song, Xiao-Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9595671/
https://www.ncbi.nlm.nih.gov/pubmed/36304956
http://dx.doi.org/10.3389/fmicb.2022.1034779
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author Zhang, Yu-Qiang
Zhang, Shan
Sun, Mei-Ling
Su, Hai-Nan
Li, Hao-Yang
Kun-Liu,
Zhang, Yu-Zhong
Chen, Xiu-Lan
Cao, Hai-Yan
Song, Xiao-Yan
author_facet Zhang, Yu-Qiang
Zhang, Shan
Sun, Mei-Ling
Su, Hai-Nan
Li, Hao-Yang
Kun-Liu,
Zhang, Yu-Zhong
Chen, Xiu-Lan
Cao, Hai-Yan
Song, Xiao-Yan
author_sort Zhang, Yu-Qiang
collection PubMed
description Bacterial leaf blight caused by Gram-negative pathogen Xanthomonas oryzae pv. oryzae (Xoo) is one of the most destructive bacterial diseases on rice. Due to the resistance, toxicity and environmental issues of chemical bactericides, new biological strategies are still in need. Although peptaibols produced by Trichoderma spp. can inhibit the growth of several Gram-positive bacteria and plant fungal pathogens, it still remains unclear whether peptaibols have anti-Xoo activity to control bacterial leaf blight on rice. In this study, we evaluated the antibacterial effects of Trichokonins A (TKA), peptaibols produced by Trichoderma longibrachiatum SMF2, against Xoo. The in vitro antibacterial activity analysis showed that the growth of Xoo was significantly inhibited by TKA, with a minimum inhibitory concentration of 54 μg/mL and that the three TKs in TKA all had remarkable anti-Xoo activity. Further inhibitory mechanism analyses revealed that TKA treatments resulted in the damage of Xoo cell morphology and the release of intracellular substances, such as proteins and nucleic acids, from Xoo cells, suggesting the damage of the permeability of Xoo cell membrane by TKA. Pathogenicity analyses showed that the lesion length on rice leaf was significantly reduced by 82.2% when treated with 27 μg/mL TKA. This study represents the first report of the antibacterial activity of peptaibols against a Gram-negative bacterium. Thus, TKA can be of a promising agent in controlling bacterial leaf blight on rice.
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spelling pubmed-95956712022-10-26 Antibacterial activity of peptaibols from Trichoderma longibrachiatum SMF2 against gram-negative Xanthomonas oryzae pv. oryzae, the causal agent of bacterial leaf blight on rice Zhang, Yu-Qiang Zhang, Shan Sun, Mei-Ling Su, Hai-Nan Li, Hao-Yang Kun-Liu, Zhang, Yu-Zhong Chen, Xiu-Lan Cao, Hai-Yan Song, Xiao-Yan Front Microbiol Microbiology Bacterial leaf blight caused by Gram-negative pathogen Xanthomonas oryzae pv. oryzae (Xoo) is one of the most destructive bacterial diseases on rice. Due to the resistance, toxicity and environmental issues of chemical bactericides, new biological strategies are still in need. Although peptaibols produced by Trichoderma spp. can inhibit the growth of several Gram-positive bacteria and plant fungal pathogens, it still remains unclear whether peptaibols have anti-Xoo activity to control bacterial leaf blight on rice. In this study, we evaluated the antibacterial effects of Trichokonins A (TKA), peptaibols produced by Trichoderma longibrachiatum SMF2, against Xoo. The in vitro antibacterial activity analysis showed that the growth of Xoo was significantly inhibited by TKA, with a minimum inhibitory concentration of 54 μg/mL and that the three TKs in TKA all had remarkable anti-Xoo activity. Further inhibitory mechanism analyses revealed that TKA treatments resulted in the damage of Xoo cell morphology and the release of intracellular substances, such as proteins and nucleic acids, from Xoo cells, suggesting the damage of the permeability of Xoo cell membrane by TKA. Pathogenicity analyses showed that the lesion length on rice leaf was significantly reduced by 82.2% when treated with 27 μg/mL TKA. This study represents the first report of the antibacterial activity of peptaibols against a Gram-negative bacterium. Thus, TKA can be of a promising agent in controlling bacterial leaf blight on rice. Frontiers Media S.A. 2022-10-11 /pmc/articles/PMC9595671/ /pubmed/36304956 http://dx.doi.org/10.3389/fmicb.2022.1034779 Text en Copyright © 2022 Zhang, Zhang, Sun, Su, Li, Kun-Liu, Zhang, Chen, Cao and Song. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Zhang, Yu-Qiang
Zhang, Shan
Sun, Mei-Ling
Su, Hai-Nan
Li, Hao-Yang
Kun-Liu,
Zhang, Yu-Zhong
Chen, Xiu-Lan
Cao, Hai-Yan
Song, Xiao-Yan
Antibacterial activity of peptaibols from Trichoderma longibrachiatum SMF2 against gram-negative Xanthomonas oryzae pv. oryzae, the causal agent of bacterial leaf blight on rice
title Antibacterial activity of peptaibols from Trichoderma longibrachiatum SMF2 against gram-negative Xanthomonas oryzae pv. oryzae, the causal agent of bacterial leaf blight on rice
title_full Antibacterial activity of peptaibols from Trichoderma longibrachiatum SMF2 against gram-negative Xanthomonas oryzae pv. oryzae, the causal agent of bacterial leaf blight on rice
title_fullStr Antibacterial activity of peptaibols from Trichoderma longibrachiatum SMF2 against gram-negative Xanthomonas oryzae pv. oryzae, the causal agent of bacterial leaf blight on rice
title_full_unstemmed Antibacterial activity of peptaibols from Trichoderma longibrachiatum SMF2 against gram-negative Xanthomonas oryzae pv. oryzae, the causal agent of bacterial leaf blight on rice
title_short Antibacterial activity of peptaibols from Trichoderma longibrachiatum SMF2 against gram-negative Xanthomonas oryzae pv. oryzae, the causal agent of bacterial leaf blight on rice
title_sort antibacterial activity of peptaibols from trichoderma longibrachiatum smf2 against gram-negative xanthomonas oryzae pv. oryzae, the causal agent of bacterial leaf blight on rice
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9595671/
https://www.ncbi.nlm.nih.gov/pubmed/36304956
http://dx.doi.org/10.3389/fmicb.2022.1034779
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