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Dental pulp stem cells retain mesenchymal phenotype despite differentiation toward retinal neuronal fate in vitro

Dental pulp stem cells (DPSCs) are an easily accessible, heterogenous source of mesenchymal stem cells (MSCs) that are derived from the neural crest. Evidence suggests that they have neurotrophic qualities in their undifferentiated state and can also be differentiated into neuronal and retinal cell...

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Autores principales: Balasankar, Aishwarya, Chan, Shu-Yi Claire, Babu, Venkata Pakala Sudheer, Yam, Gary, Tin, Goh Bee, Singhal, Shweta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9596784/
https://www.ncbi.nlm.nih.gov/pubmed/36314029
http://dx.doi.org/10.3389/fmed.2022.821361
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author Balasankar, Aishwarya
Chan, Shu-Yi Claire
Babu, Venkata Pakala Sudheer
Yam, Gary
Tin, Goh Bee
Singhal, Shweta
author_facet Balasankar, Aishwarya
Chan, Shu-Yi Claire
Babu, Venkata Pakala Sudheer
Yam, Gary
Tin, Goh Bee
Singhal, Shweta
author_sort Balasankar, Aishwarya
collection PubMed
description Dental pulp stem cells (DPSCs) are an easily accessible, heterogenous source of mesenchymal stem cells (MSCs) that are derived from the neural crest. Evidence suggests that they have neurotrophic qualities in their undifferentiated state and can also be differentiated into neuronal and retinal cell types. There is growing interest in using DPSCs in cell-based therapies to treat glaucoma and blinding retinal diseases. However, careful characterization of these cells is necessary as direct intravitreal and subretinal MSC transplantation is known to lead to deleterious glial reaction and fibrosis. In this study, we provide evidence for the mesenchymal-predominant nature of DPSCs and show that DPSCs maintain their mesenchymal phenotype despite upregulating mature retinal markers under retinal differentiation conditions. CD56, which was previously thought to be a specific marker of neural crest lineage, is robustly co-expressed with mesenchymal markers and may not be adequate for isolating a subpopulation of neural crest cells in DPSCs. Therefore, identification of more specific markers is required to elucidate the heterogeneity of the population and to successfully isolate a putative neural stem cell population before DPSCs can be used for retinal therapy.
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spelling pubmed-95967842022-10-27 Dental pulp stem cells retain mesenchymal phenotype despite differentiation toward retinal neuronal fate in vitro Balasankar, Aishwarya Chan, Shu-Yi Claire Babu, Venkata Pakala Sudheer Yam, Gary Tin, Goh Bee Singhal, Shweta Front Med (Lausanne) Medicine Dental pulp stem cells (DPSCs) are an easily accessible, heterogenous source of mesenchymal stem cells (MSCs) that are derived from the neural crest. Evidence suggests that they have neurotrophic qualities in their undifferentiated state and can also be differentiated into neuronal and retinal cell types. There is growing interest in using DPSCs in cell-based therapies to treat glaucoma and blinding retinal diseases. However, careful characterization of these cells is necessary as direct intravitreal and subretinal MSC transplantation is known to lead to deleterious glial reaction and fibrosis. In this study, we provide evidence for the mesenchymal-predominant nature of DPSCs and show that DPSCs maintain their mesenchymal phenotype despite upregulating mature retinal markers under retinal differentiation conditions. CD56, which was previously thought to be a specific marker of neural crest lineage, is robustly co-expressed with mesenchymal markers and may not be adequate for isolating a subpopulation of neural crest cells in DPSCs. Therefore, identification of more specific markers is required to elucidate the heterogeneity of the population and to successfully isolate a putative neural stem cell population before DPSCs can be used for retinal therapy. Frontiers Media S.A. 2022-10-12 /pmc/articles/PMC9596784/ /pubmed/36314029 http://dx.doi.org/10.3389/fmed.2022.821361 Text en Copyright © 2022 Balasankar, Chan, Babu, Yam, Tin and Singhal. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Medicine
Balasankar, Aishwarya
Chan, Shu-Yi Claire
Babu, Venkata Pakala Sudheer
Yam, Gary
Tin, Goh Bee
Singhal, Shweta
Dental pulp stem cells retain mesenchymal phenotype despite differentiation toward retinal neuronal fate in vitro
title Dental pulp stem cells retain mesenchymal phenotype despite differentiation toward retinal neuronal fate in vitro
title_full Dental pulp stem cells retain mesenchymal phenotype despite differentiation toward retinal neuronal fate in vitro
title_fullStr Dental pulp stem cells retain mesenchymal phenotype despite differentiation toward retinal neuronal fate in vitro
title_full_unstemmed Dental pulp stem cells retain mesenchymal phenotype despite differentiation toward retinal neuronal fate in vitro
title_short Dental pulp stem cells retain mesenchymal phenotype despite differentiation toward retinal neuronal fate in vitro
title_sort dental pulp stem cells retain mesenchymal phenotype despite differentiation toward retinal neuronal fate in vitro
topic Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9596784/
https://www.ncbi.nlm.nih.gov/pubmed/36314029
http://dx.doi.org/10.3389/fmed.2022.821361
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