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Covalent immobilization of VEGF on allogeneic bone through polydopamine coating to improve bone regeneration

Objective: Promoting bone regeneration and repairing in bone defects is of great significance in clinical work. Using a simple and effective surface treatment method to enhance the osteogenic ability of existing bone scaffold is a promising method. In this article, we study the application of catech...

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Autores principales: Huang, Jianhao, Lu, Jingwei, Liu, Ziying, Jin, Jing, Xie, Chunmei, Zheng, Yang, Wang, Zhen, Yu, Lingfeng, Zhu, Yan, Fan, Gentao, Sun, Guojing, Xu, Zhihong, Zhou, Guangxin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9597090/
https://www.ncbi.nlm.nih.gov/pubmed/36312529
http://dx.doi.org/10.3389/fbioe.2022.1003677
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author Huang, Jianhao
Lu, Jingwei
Liu, Ziying
Jin, Jing
Xie, Chunmei
Zheng, Yang
Wang, Zhen
Yu, Lingfeng
Zhu, Yan
Fan, Gentao
Sun, Guojing
Xu, Zhihong
Zhou, Guangxin
author_facet Huang, Jianhao
Lu, Jingwei
Liu, Ziying
Jin, Jing
Xie, Chunmei
Zheng, Yang
Wang, Zhen
Yu, Lingfeng
Zhu, Yan
Fan, Gentao
Sun, Guojing
Xu, Zhihong
Zhou, Guangxin
author_sort Huang, Jianhao
collection PubMed
description Objective: Promoting bone regeneration and repairing in bone defects is of great significance in clinical work. Using a simple and effective surface treatment method to enhance the osteogenic ability of existing bone scaffold is a promising method. In this article, we study the application of catecholic amino acid 3,4-dihydroxyphenylalanine (DOPA) surface coating chelated with vascular endothelial growth factor (VEGF) on allogeneic bone. Method: Allogeneic bone is immersed in DOPA solution and DOPA form polydopamine (PDA) with good adhesion. Electron microscopy is used to characterize the surface characteristics of allogeneic bone. MC3T3-E1 cells were tested for biocompatibility and osteogenic signal expression. Finally, a 12-week rabbit bone defect model was established to evaluate bone regeneration capability. Results: We found that the surface microenvironment of DOPA bonded allogeneic bone was similar to the natural allogeneic bone. VEGF loaded allografts exhibited satisfying biocompatibility and promoted the expression of osteogenic related signals in vitro. The VEGF loaded allografts healed the bone defect after 12 weeks of implantation that continuous and intact bone cortex was observed. Conclusion: The PDA coating is a simple surface modification method and has mild properties and high adhesion. Meanwhile, the PDA coating can act on the surface modification of different materials. This study provides an efficient surface modification method for enhancing bone regeneration by PDA coating, which has a high potential for translational clinical applications.
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spelling pubmed-95970902022-10-27 Covalent immobilization of VEGF on allogeneic bone through polydopamine coating to improve bone regeneration Huang, Jianhao Lu, Jingwei Liu, Ziying Jin, Jing Xie, Chunmei Zheng, Yang Wang, Zhen Yu, Lingfeng Zhu, Yan Fan, Gentao Sun, Guojing Xu, Zhihong Zhou, Guangxin Front Bioeng Biotechnol Bioengineering and Biotechnology Objective: Promoting bone regeneration and repairing in bone defects is of great significance in clinical work. Using a simple and effective surface treatment method to enhance the osteogenic ability of existing bone scaffold is a promising method. In this article, we study the application of catecholic amino acid 3,4-dihydroxyphenylalanine (DOPA) surface coating chelated with vascular endothelial growth factor (VEGF) on allogeneic bone. Method: Allogeneic bone is immersed in DOPA solution and DOPA form polydopamine (PDA) with good adhesion. Electron microscopy is used to characterize the surface characteristics of allogeneic bone. MC3T3-E1 cells were tested for biocompatibility and osteogenic signal expression. Finally, a 12-week rabbit bone defect model was established to evaluate bone regeneration capability. Results: We found that the surface microenvironment of DOPA bonded allogeneic bone was similar to the natural allogeneic bone. VEGF loaded allografts exhibited satisfying biocompatibility and promoted the expression of osteogenic related signals in vitro. The VEGF loaded allografts healed the bone defect after 12 weeks of implantation that continuous and intact bone cortex was observed. Conclusion: The PDA coating is a simple surface modification method and has mild properties and high adhesion. Meanwhile, the PDA coating can act on the surface modification of different materials. This study provides an efficient surface modification method for enhancing bone regeneration by PDA coating, which has a high potential for translational clinical applications. Frontiers Media S.A. 2022-10-12 /pmc/articles/PMC9597090/ /pubmed/36312529 http://dx.doi.org/10.3389/fbioe.2022.1003677 Text en Copyright © 2022 Huang, Lu, Liu, Jin, Xie, Zheng, Wang, Yu, Zhu, Fan, Sun, Xu and Zhou. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Huang, Jianhao
Lu, Jingwei
Liu, Ziying
Jin, Jing
Xie, Chunmei
Zheng, Yang
Wang, Zhen
Yu, Lingfeng
Zhu, Yan
Fan, Gentao
Sun, Guojing
Xu, Zhihong
Zhou, Guangxin
Covalent immobilization of VEGF on allogeneic bone through polydopamine coating to improve bone regeneration
title Covalent immobilization of VEGF on allogeneic bone through polydopamine coating to improve bone regeneration
title_full Covalent immobilization of VEGF on allogeneic bone through polydopamine coating to improve bone regeneration
title_fullStr Covalent immobilization of VEGF on allogeneic bone through polydopamine coating to improve bone regeneration
title_full_unstemmed Covalent immobilization of VEGF on allogeneic bone through polydopamine coating to improve bone regeneration
title_short Covalent immobilization of VEGF on allogeneic bone through polydopamine coating to improve bone regeneration
title_sort covalent immobilization of vegf on allogeneic bone through polydopamine coating to improve bone regeneration
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9597090/
https://www.ncbi.nlm.nih.gov/pubmed/36312529
http://dx.doi.org/10.3389/fbioe.2022.1003677
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