Cargando…

Characterization of WOX genes revealed drought tolerance, callus induction, and tissue regeneration in Gossypium hirsutum

Cotton is an important natural fiber crop; its seeds are the main oil source. Abiotic stresses cause a significant decline in its production. The WUSCHEL-related Homeobox (WOX) genes have been involved in plant growth, development, and stress responses. However, the functions of WOX genes are less k...

Descripción completa

Detalles Bibliográficos
Autores principales: Muhammad Tajo, Sani, Pan, Zhaoe, He, Shoupu, Chen, Baojun, KM, Yusuf, Mahmood, Tahir, Bello Sadau, Salisu, Shahid Iqbal, Muhammad, Gereziher, Teame, Suleiman Abubakar, Umar, Joseph, Masha, Sammani, Tajo, Geng, Xiaoli, Du, Xiongming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9597092/
https://www.ncbi.nlm.nih.gov/pubmed/36313437
http://dx.doi.org/10.3389/fgene.2022.928055
_version_ 1784816016393502720
author Muhammad Tajo, Sani
Pan, Zhaoe
He, Shoupu
Chen, Baojun
KM, Yusuf
Mahmood, Tahir
Bello Sadau, Salisu
Shahid Iqbal, Muhammad
Gereziher, Teame
Suleiman Abubakar, Umar
Joseph, Masha
Sammani, Tajo
Geng, Xiaoli
Du, Xiongming
author_facet Muhammad Tajo, Sani
Pan, Zhaoe
He, Shoupu
Chen, Baojun
KM, Yusuf
Mahmood, Tahir
Bello Sadau, Salisu
Shahid Iqbal, Muhammad
Gereziher, Teame
Suleiman Abubakar, Umar
Joseph, Masha
Sammani, Tajo
Geng, Xiaoli
Du, Xiongming
author_sort Muhammad Tajo, Sani
collection PubMed
description Cotton is an important natural fiber crop; its seeds are the main oil source. Abiotic stresses cause a significant decline in its production. The WUSCHEL-related Homeobox (WOX) genes have been involved in plant growth, development, and stress responses. However, the functions of WOX genes are less known in cotton. This study identified 39, 40, 21, and 20 WOX genes in Gossypium hirsutum, Gossypium barbadense, Gossypium arboreum, and Gossypium raimondii, respectively. All the WOX genes in four cotton species could be classified into three clades, which is consistent with previous research. The gene structure and conserved domain of all WOX genes were analyzed. The expressions of WOX genes in germinating hypocotyls and callus were characterized, and it was found that most genes were up-regulated. One candidate gene Gh_ A01G127500 was selected to perform the virus-induced gene silencing (VIGS) experiment, and it was found that the growth of the silenced plant (pCLCrVA: GhWOX4_A01) was significantly inhibited compared with the wild type. In the silenced plant, there is an increase in antioxidant activities and a decrease in oxidant activities compared with the control plant. In physiological analysis, the relative electrolyte leakage level and the excised leaf water loss of the infected plant were increased. Still, both the relative leaf water content and the chlorophyll content were decreased. This study proved that WOX genes play important roles in drought stress and callus induction, but more work must be performed to address the molecular functions of WOX genes.
format Online
Article
Text
id pubmed-9597092
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-95970922022-10-27 Characterization of WOX genes revealed drought tolerance, callus induction, and tissue regeneration in Gossypium hirsutum Muhammad Tajo, Sani Pan, Zhaoe He, Shoupu Chen, Baojun KM, Yusuf Mahmood, Tahir Bello Sadau, Salisu Shahid Iqbal, Muhammad Gereziher, Teame Suleiman Abubakar, Umar Joseph, Masha Sammani, Tajo Geng, Xiaoli Du, Xiongming Front Genet Genetics Cotton is an important natural fiber crop; its seeds are the main oil source. Abiotic stresses cause a significant decline in its production. The WUSCHEL-related Homeobox (WOX) genes have been involved in plant growth, development, and stress responses. However, the functions of WOX genes are less known in cotton. This study identified 39, 40, 21, and 20 WOX genes in Gossypium hirsutum, Gossypium barbadense, Gossypium arboreum, and Gossypium raimondii, respectively. All the WOX genes in four cotton species could be classified into three clades, which is consistent with previous research. The gene structure and conserved domain of all WOX genes were analyzed. The expressions of WOX genes in germinating hypocotyls and callus were characterized, and it was found that most genes were up-regulated. One candidate gene Gh_ A01G127500 was selected to perform the virus-induced gene silencing (VIGS) experiment, and it was found that the growth of the silenced plant (pCLCrVA: GhWOX4_A01) was significantly inhibited compared with the wild type. In the silenced plant, there is an increase in antioxidant activities and a decrease in oxidant activities compared with the control plant. In physiological analysis, the relative electrolyte leakage level and the excised leaf water loss of the infected plant were increased. Still, both the relative leaf water content and the chlorophyll content were decreased. This study proved that WOX genes play important roles in drought stress and callus induction, but more work must be performed to address the molecular functions of WOX genes. Frontiers Media S.A. 2022-10-12 /pmc/articles/PMC9597092/ /pubmed/36313437 http://dx.doi.org/10.3389/fgene.2022.928055 Text en Copyright © 2022 Muhammad Tajo, Pan, He, Chen, KM, Mahmood, Bello Sadau, Shahid Iqbal, Gereziher, Suleiman Abubakar, Joseph, Sammani, Geng and Du. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Muhammad Tajo, Sani
Pan, Zhaoe
He, Shoupu
Chen, Baojun
KM, Yusuf
Mahmood, Tahir
Bello Sadau, Salisu
Shahid Iqbal, Muhammad
Gereziher, Teame
Suleiman Abubakar, Umar
Joseph, Masha
Sammani, Tajo
Geng, Xiaoli
Du, Xiongming
Characterization of WOX genes revealed drought tolerance, callus induction, and tissue regeneration in Gossypium hirsutum
title Characterization of WOX genes revealed drought tolerance, callus induction, and tissue regeneration in Gossypium hirsutum
title_full Characterization of WOX genes revealed drought tolerance, callus induction, and tissue regeneration in Gossypium hirsutum
title_fullStr Characterization of WOX genes revealed drought tolerance, callus induction, and tissue regeneration in Gossypium hirsutum
title_full_unstemmed Characterization of WOX genes revealed drought tolerance, callus induction, and tissue regeneration in Gossypium hirsutum
title_short Characterization of WOX genes revealed drought tolerance, callus induction, and tissue regeneration in Gossypium hirsutum
title_sort characterization of wox genes revealed drought tolerance, callus induction, and tissue regeneration in gossypium hirsutum
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9597092/
https://www.ncbi.nlm.nih.gov/pubmed/36313437
http://dx.doi.org/10.3389/fgene.2022.928055
work_keys_str_mv AT muhammadtajosani characterizationofwoxgenesrevealeddroughttolerancecallusinductionandtissueregenerationingossypiumhirsutum
AT panzhaoe characterizationofwoxgenesrevealeddroughttolerancecallusinductionandtissueregenerationingossypiumhirsutum
AT heshoupu characterizationofwoxgenesrevealeddroughttolerancecallusinductionandtissueregenerationingossypiumhirsutum
AT chenbaojun characterizationofwoxgenesrevealeddroughttolerancecallusinductionandtissueregenerationingossypiumhirsutum
AT kmyusuf characterizationofwoxgenesrevealeddroughttolerancecallusinductionandtissueregenerationingossypiumhirsutum
AT mahmoodtahir characterizationofwoxgenesrevealeddroughttolerancecallusinductionandtissueregenerationingossypiumhirsutum
AT bellosadausalisu characterizationofwoxgenesrevealeddroughttolerancecallusinductionandtissueregenerationingossypiumhirsutum
AT shahidiqbalmuhammad characterizationofwoxgenesrevealeddroughttolerancecallusinductionandtissueregenerationingossypiumhirsutum
AT gereziherteame characterizationofwoxgenesrevealeddroughttolerancecallusinductionandtissueregenerationingossypiumhirsutum
AT suleimanabubakarumar characterizationofwoxgenesrevealeddroughttolerancecallusinductionandtissueregenerationingossypiumhirsutum
AT josephmasha characterizationofwoxgenesrevealeddroughttolerancecallusinductionandtissueregenerationingossypiumhirsutum
AT sammanitajo characterizationofwoxgenesrevealeddroughttolerancecallusinductionandtissueregenerationingossypiumhirsutum
AT gengxiaoli characterizationofwoxgenesrevealeddroughttolerancecallusinductionandtissueregenerationingossypiumhirsutum
AT duxiongming characterizationofwoxgenesrevealeddroughttolerancecallusinductionandtissueregenerationingossypiumhirsutum