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Microbiota DNA isolation, 16S rRNA amplicon sequencing, and bioinformatic analysis for bacterial microbiome profiling of rodent fecal samples

Fecal samples are frequently used to characterize bacterial populations of the gastrointestinal tract. A protocol is provided to profile gut bacterial populations using rodent fecal samples. We describe the optimal procedures for collecting rodent fecal samples, isolating genomic DNA, 16S rRNA gene...

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Detalles Bibliográficos
Autores principales:	Love, Chloe J., Gubert, Carolina, Kodikara, Saritha, Kong, Geraldine, Lê Cao, Kim-Anh, Hannan, Anthony J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9597187/ https://www.ncbi.nlm.nih.gov/pubmed/36313541 http://dx.doi.org/10.1016/j.xpro.2022.101772

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