Cargando…

Microbiota DNA isolation, 16S rRNA amplicon sequencing, and bioinformatic analysis for bacterial microbiome profiling of rodent fecal samples

Fecal samples are frequently used to characterize bacterial populations of the gastrointestinal tract. A protocol is provided to profile gut bacterial populations using rodent fecal samples. We describe the optimal procedures for collecting rodent fecal samples, isolating genomic DNA, 16S rRNA gene...

Descripción completa

Detalles Bibliográficos
Autores principales:	Love, Chloe J., Gubert, Carolina, Kodikara, Saritha, Kong, Geraldine, Lê Cao, Kim-Anh, Hannan, Anthony J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9597187/ https://www.ncbi.nlm.nih.gov/pubmed/36313541 http://dx.doi.org/10.1016/j.xpro.2022.101772

Ejemplares similares

Gene-environment-gut interactions in Huntington's disease mice are associated with environmental modulation of the gut microbiome
por: Gubert, Carolina, et al.
Publicado: (2021)

Comparing bioinformatic pipelines for microbial 16S rRNA amplicon sequencing
por: Prodan, Andrei, et al.
Publicado: (2020)

Comparison of Bioinformatics Pipelines and Operating Systems for the Analyses of 16S rRNA Gene Amplicon Sequences in Human Fecal Samples
por: Marizzoni, Moira, et al.
Publicado: (2020)

Faecal microbiota transplant ameliorates gut dysbiosis and cognitive deficits in Huntington’s disease mice
por: Gubert, Carolina, et al.
Publicado: (2022)

Identification of the microbial diversity after fecal microbiota transplantation therapy for chronic intractable constipation using 16s rRNA amplicon sequencing
por: Ohara, Tadashi
Publicado: (2019)

MinION™ Nanopore Sequencing of Skin Microbiome 16S and 16S-23S rRNA Gene Amplicons
por: Rozas, Miquel, et al.
Publicado: (2022)

Comparison of Mothur and QIIME for the Analysis of Rumen Microbiota Composition Based on 16S rRNA Amplicon Sequences
por: López-García, Adrian, et al.
Publicado: (2018)

Analysis of the mouse gut microbiome using full-length 16S rRNA amplicon sequencing
por: Shin, Jongoh, et al.
Publicado: (2016)

MetaSquare: an integrated metadatabase of 16S rRNA gene amplicon for microbiome taxonomic classification
por: Liao, Chun-Chieh, et al.
Publicado: (2022)

Utility of a portable desiccant system for preservation of fecal samples for downstream 16S rRNA amplicon sequencing
por: Johnson, Philip J., et al.
Publicado: (2018)

Statistical challenges in longitudinal microbiome data analysis
por: Kodikara, Saritha, et al.
Publicado: (2022)

Rabbit microbiota across the whole body revealed by 16S rRNA gene amplicon sequencing
por: Hu, Xiaofen, et al.
Publicado: (2021)

Species-Level Resolution of Female Bladder Microbiota from 16S rRNA Amplicon Sequencing
por: Hoffman, Carter, et al.
Publicado: (2021)

High-Resolution Differentiation of Enteric Bacteria in Premature Infant Fecal Microbiomes Using a Novel rRNA Amplicon
por: Graf, J., et al.
Publicado: (2021)

Correction to: MetaSquare: an integrated metadatabase of 16S rRNA gene amplicon for microbiome taxonomic classification
Publicado: (2022)

Reproductive Microbiomes in Domestic Livestock: Insights Utilizing 16S rRNA Gene Amplicon Community Sequencing
por: Poole, Rebecca K., et al.
Publicado: (2023)

Best practices for generating and analyzing 16S rRNA amplicon data to track coral microbiome dynamics
por: Silva, Denise P., et al.
Publicado: (2023)

Comprehensive Assessment of 16S rRNA Gene Amplicon Sequencing for Microbiome Profiling across Multiple Habitats
por: Zhang, Wenke, et al.
Publicado: (2023)

Metaproteomic and 16S rRNA Gene Sequencing Analysis of the Infant Fecal Microbiome
por: Cortes, Laetitia, et al.
Publicado: (2019)

Up to Species-level Community Analysis of Human Gut Microbiota by 16S rRNA Amplicon Pyrosequencing
por: NAKAYAMA, Jiro, et al.
Publicado: (2013)

Apical periodontitis: preliminary assessment of microbiota by 16S rRNA high throughput amplicon target sequencing
por: Mussano, Federico, et al.
Publicado: (2018)

The Effect of Common Viral Inactivation Techniques on 16S rRNA Amplicon-Based Analysis of the Gut Microbiota
por: McAdams, Zachary, et al.
Publicado: (2021)

16S rRNA Gene Amplicon Sequencing of Gut Microbiota Affected by Four Probiotic Strains in Mice
por: Ren, Jianwei, et al.
Publicado: (2023)

Comparison of paperpoint and curette sampling of subgingival microbiome composition as analyzed by 16S rRNA gene amplicon sequencing
por: Beyer, K., et al.
Publicado: (2017)

Gut Microbiome 16S rRNA Gene Amplicon Taxonomic Profiling of Hospitalized Moroccan COVID-19 Patients
por: Sehli, Sofia, et al.
Publicado: (2022)

16S rRNA Amplicon Sequencing for Epidemiological Surveys of Bacteria in Wildlife
por: Galan, Maxime, et al.
Publicado: (2016)

Pyrosequencing of 16S rRNA gene amplicons to study the microbiota in the gastrointestinal tract of carp (Cyprinus carpio L.)
por: van Kessel, Maartje AHJ, et al.
Publicado: (2011)

The Effect of Lactobacillus plantarum BW2013 on The Gut Microbiota in Mice Analyzed by 16S rRNA Amplicon Sequencing
por: TONG, TONG, et al.
Publicado: (2021)

Comparison of Intestinal Microbiota Between Healthy and MMVD Chihuahuas Using 16S rRNA Gene Amplicon Sequencing
por: Araki, Ryuji, et al.
Publicado: (2022)

High-Throughput Miniaturized 16S rRNA Amplicon Library Preparation Reduces Costs while Preserving Microbiome Integrity
por: Minich, Jeremiah J., et al.
Publicado: (2018)

Microbiome Shift, Diversity, and Overabundance of Opportunistic Pathogens in Bovine Digital Dermatitis Revealed by 16S rRNA Amplicon Sequencing
por: Espiritu, Hector M., et al.
Publicado: (2020)

Curated and harmonized gut microbiome 16S rRNA amplicon data from dietary fiber intervention studies in humans
por: Rodriguez, Cynthia I., et al.
Publicado: (2023)

16S rRNA gene amplicon sequencing of gut microbiota in gestational diabetes mellitus and their correlation with disease risk factors
por: Wei, J., et al.
Publicado: (2021)

Exploring Gut Microbiota in Patients with Colorectal Disease Based on 16S rRNA Gene Amplicon and Shallow Metagenomic Sequencing
por: Liu, Yuanfeng, et al.
Publicado: (2021)

High-throughput qPCR and 16S rRNA gene amplicon sequencing as complementary methods for the investigation of the cheese microbiota
por: Dreier, Matthias, et al.
Publicado: (2022)

Sediment Microbiota in Response to Circuitry of Sediment Microbial Fuel Cells, Revealed by 16S rRNA Gene Amplicon Sequencing
por: Takemura, Yasuyuki, et al.
Publicado: (2021)

Exploring thematic structure and predicted functionality of 16S rRNA amplicon data
por: Woloszynek, Stephen, et al.
Publicado: (2019)

Processing human urine and ureteral stents for 16S rRNA amplicon sequencing
por: Al, Kait F., et al.
Publicado: (2021)

Complementing 16S rRNA Gene Amplicon Sequencing with Total Bacterial Load To Infer Absolute Species Concentrations in the Vaginal Microbiome
por: Tettamanti Boshier, Florencia A., et al.
Publicado: (2020)

Establishment and assessment of an amplicon sequencing method targeting the 16S-ITS-23S rRNA operon for analysis of the equine gut microbiome
por: Kinoshita, Yuta, et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_0u5aa7r95ashrg7ga9el0gono5