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Infusion of an acidified ethanolic—dextrose solution enhances urinary ammonium excretion and increases acid resilience in non—mechanically ventilated acidotic rabbits

Hitherto, the rabbit has long been known to have a very poor tolerance to non—volatile acid. In this study, we tested the hypothesis that acid resilience in the acidotic rabbit can be increased by enhancing the plasma availability of a naturally occurring volatile fatty acid, namely acetate. To asce...

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Autor principal: Walsh, Patrick A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9597196/
https://www.ncbi.nlm.nih.gov/pubmed/36311228
http://dx.doi.org/10.3389/fphys.2022.860042
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author Walsh, Patrick A.
author_facet Walsh, Patrick A.
author_sort Walsh, Patrick A.
collection PubMed
description Hitherto, the rabbit has long been known to have a very poor tolerance to non—volatile acid. In this study, we tested the hypothesis that acid resilience in the acidotic rabbit can be increased by enhancing the plasma availability of a naturally occurring volatile fatty acid, namely acetate. To ascertain the relative merits of the respiratory and renal systems in contributing to that resilience, we conducted our studies in non—ventilated and mechanically ventilated acidotic animals. Using ethanol as a feeder of acetate, and to counteract the antidiuretic effects of surgical interventions, we induced acidosis in anaesthetised rabbits, by intravenously infusing an acidified ethanolic dextrose solution. We observed very potent respiratory regulation of arterial blood pH coupled with a notable renal response by way of a 25-fold increase in urinary ammonium excretion in the non—ventilated group. In contrast, arterial blood pH plummeted much more rapidly in the mechanically—ventilated animals, but the compensated renal response was enormous, in the form of an 85 -fold increase in urinary ammonium output. Despite this significant adaptive renal response, the non -mechanically ventilated group of rabbits showed the greater acid resilience. This was attributed to an acetate stimulated flux through a series of metabolic pathways, generating supplementary buffer in the form of bicarbonate and ammonia, complemented by a robust respiratory response.
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spelling pubmed-95971962022-10-27 Infusion of an acidified ethanolic—dextrose solution enhances urinary ammonium excretion and increases acid resilience in non—mechanically ventilated acidotic rabbits Walsh, Patrick A. Front Physiol Physiology Hitherto, the rabbit has long been known to have a very poor tolerance to non—volatile acid. In this study, we tested the hypothesis that acid resilience in the acidotic rabbit can be increased by enhancing the plasma availability of a naturally occurring volatile fatty acid, namely acetate. To ascertain the relative merits of the respiratory and renal systems in contributing to that resilience, we conducted our studies in non—ventilated and mechanically ventilated acidotic animals. Using ethanol as a feeder of acetate, and to counteract the antidiuretic effects of surgical interventions, we induced acidosis in anaesthetised rabbits, by intravenously infusing an acidified ethanolic dextrose solution. We observed very potent respiratory regulation of arterial blood pH coupled with a notable renal response by way of a 25-fold increase in urinary ammonium excretion in the non—ventilated group. In contrast, arterial blood pH plummeted much more rapidly in the mechanically—ventilated animals, but the compensated renal response was enormous, in the form of an 85 -fold increase in urinary ammonium output. Despite this significant adaptive renal response, the non -mechanically ventilated group of rabbits showed the greater acid resilience. This was attributed to an acetate stimulated flux through a series of metabolic pathways, generating supplementary buffer in the form of bicarbonate and ammonia, complemented by a robust respiratory response. Frontiers Media S.A. 2022-10-12 /pmc/articles/PMC9597196/ /pubmed/36311228 http://dx.doi.org/10.3389/fphys.2022.860042 Text en Copyright © 2022 Walsh. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Walsh, Patrick A.
Infusion of an acidified ethanolic—dextrose solution enhances urinary ammonium excretion and increases acid resilience in non—mechanically ventilated acidotic rabbits
title Infusion of an acidified ethanolic—dextrose solution enhances urinary ammonium excretion and increases acid resilience in non—mechanically ventilated acidotic rabbits
title_full Infusion of an acidified ethanolic—dextrose solution enhances urinary ammonium excretion and increases acid resilience in non—mechanically ventilated acidotic rabbits
title_fullStr Infusion of an acidified ethanolic—dextrose solution enhances urinary ammonium excretion and increases acid resilience in non—mechanically ventilated acidotic rabbits
title_full_unstemmed Infusion of an acidified ethanolic—dextrose solution enhances urinary ammonium excretion and increases acid resilience in non—mechanically ventilated acidotic rabbits
title_short Infusion of an acidified ethanolic—dextrose solution enhances urinary ammonium excretion and increases acid resilience in non—mechanically ventilated acidotic rabbits
title_sort infusion of an acidified ethanolic—dextrose solution enhances urinary ammonium excretion and increases acid resilience in non—mechanically ventilated acidotic rabbits
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9597196/
https://www.ncbi.nlm.nih.gov/pubmed/36311228
http://dx.doi.org/10.3389/fphys.2022.860042
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