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PEG-induced physiological drought for screening winter wheat genotypes sensitivity – integrated biochemical and chlorophyll a fluorescence analysis

This study aimed to screen different winter wheat genotypes at the onset of metabolic changes induced by water deficit to comprehend possible adaptive features of photosynthetic apparatus function and structure to physiological drought. The drought treatment was the most influential variable affecti...

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Autores principales: Peršić, Vesna, Ament, Anita, Antunović Dunić, Jasenka, Drezner, Georg, Cesar, Vera
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9597320/
https://www.ncbi.nlm.nih.gov/pubmed/36311092
http://dx.doi.org/10.3389/fpls.2022.987702
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author Peršić, Vesna
Ament, Anita
Antunović Dunić, Jasenka
Drezner, Georg
Cesar, Vera
author_facet Peršić, Vesna
Ament, Anita
Antunović Dunić, Jasenka
Drezner, Georg
Cesar, Vera
author_sort Peršić, Vesna
collection PubMed
description This study aimed to screen different winter wheat genotypes at the onset of metabolic changes induced by water deficit to comprehend possible adaptive features of photosynthetic apparatus function and structure to physiological drought. The drought treatment was the most influential variable affecting plant growth and relative water content, and genotype variability determined with what intensity varieties of winter wheat seedlings responded to water deficit. PEG-induced drought, as expected, changed phenomenological energy fluxes and the efficiency with which an electron is transferred to final PSI acceptors. Based on the effect size, fluorescence parameters were grouped to represent photochemical parameters, that is, the donor and acceptor side of PSII (PC1); the thermal phase of the photosynthetic process, or the electron flow around PSI, and the chain of electrons between PSII and PSI (PC2); and phenomenological energy fluxes per cross-section (PC3). Furthermore, four distinct clusters of genotypes were discerned based on their response to imposed physiological drought, and integrated analysis enabled an explanation of their reactions’ specificity. The most reliable JIP-test parameters for detecting and comparing the drought impact among tested genotypes were the variable fluorescence at K, L, I step, and PI(TOT). To conclude, developing and improving screening methods for identifying and evaluating functional relationships of relevant characteristics that are useful for acclimation, acclimatization, and adaptation to different types of drought stress can contribute to the progress in breeding research of winter wheat drought-tolerant lines.
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spelling pubmed-95973202022-10-27 PEG-induced physiological drought for screening winter wheat genotypes sensitivity – integrated biochemical and chlorophyll a fluorescence analysis Peršić, Vesna Ament, Anita Antunović Dunić, Jasenka Drezner, Georg Cesar, Vera Front Plant Sci Plant Science This study aimed to screen different winter wheat genotypes at the onset of metabolic changes induced by water deficit to comprehend possible adaptive features of photosynthetic apparatus function and structure to physiological drought. The drought treatment was the most influential variable affecting plant growth and relative water content, and genotype variability determined with what intensity varieties of winter wheat seedlings responded to water deficit. PEG-induced drought, as expected, changed phenomenological energy fluxes and the efficiency with which an electron is transferred to final PSI acceptors. Based on the effect size, fluorescence parameters were grouped to represent photochemical parameters, that is, the donor and acceptor side of PSII (PC1); the thermal phase of the photosynthetic process, or the electron flow around PSI, and the chain of electrons between PSII and PSI (PC2); and phenomenological energy fluxes per cross-section (PC3). Furthermore, four distinct clusters of genotypes were discerned based on their response to imposed physiological drought, and integrated analysis enabled an explanation of their reactions’ specificity. The most reliable JIP-test parameters for detecting and comparing the drought impact among tested genotypes were the variable fluorescence at K, L, I step, and PI(TOT). To conclude, developing and improving screening methods for identifying and evaluating functional relationships of relevant characteristics that are useful for acclimation, acclimatization, and adaptation to different types of drought stress can contribute to the progress in breeding research of winter wheat drought-tolerant lines. Frontiers Media S.A. 2022-10-12 /pmc/articles/PMC9597320/ /pubmed/36311092 http://dx.doi.org/10.3389/fpls.2022.987702 Text en Copyright © 2022 Peršić, Ament, Antunović Dunić, Drezner and Cesar https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Peršić, Vesna
Ament, Anita
Antunović Dunić, Jasenka
Drezner, Georg
Cesar, Vera
PEG-induced physiological drought for screening winter wheat genotypes sensitivity – integrated biochemical and chlorophyll a fluorescence analysis
title PEG-induced physiological drought for screening winter wheat genotypes sensitivity – integrated biochemical and chlorophyll a fluorescence analysis
title_full PEG-induced physiological drought for screening winter wheat genotypes sensitivity – integrated biochemical and chlorophyll a fluorescence analysis
title_fullStr PEG-induced physiological drought for screening winter wheat genotypes sensitivity – integrated biochemical and chlorophyll a fluorescence analysis
title_full_unstemmed PEG-induced physiological drought for screening winter wheat genotypes sensitivity – integrated biochemical and chlorophyll a fluorescence analysis
title_short PEG-induced physiological drought for screening winter wheat genotypes sensitivity – integrated biochemical and chlorophyll a fluorescence analysis
title_sort peg-induced physiological drought for screening winter wheat genotypes sensitivity – integrated biochemical and chlorophyll a fluorescence analysis
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9597320/
https://www.ncbi.nlm.nih.gov/pubmed/36311092
http://dx.doi.org/10.3389/fpls.2022.987702
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