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DNase inhibits early biofilm formation in Pseudomonas aeruginosa- or Staphylococcus aureus-induced empyema models

Anti-infection strategies against pleural empyema include the use of antibiotics and drainage treatments, but bacterial eradication rates remain low. A major challenge is the formation of biofilms in the pleural cavity. DNase has antibiofilm efficacy in vitro, and intrapleural therapy with DNase is...

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Autores principales: Deng, Wusheng, Lei, Yanmei, Tang, Xiujia, Li, Dingbin, Liang, Jinhua, Luo, Jing, Liu, Liuyuan, Zhang, Wenshu, Ye, Liumei, Kong, Jinliang, Wang, Ke, Chen, Zhaoyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9597695/
https://www.ncbi.nlm.nih.gov/pubmed/36310876
http://dx.doi.org/10.3389/fcimb.2022.917038
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author Deng, Wusheng
Lei, Yanmei
Tang, Xiujia
Li, Dingbin
Liang, Jinhua
Luo, Jing
Liu, Liuyuan
Zhang, Wenshu
Ye, Liumei
Kong, Jinliang
Wang, Ke
Chen, Zhaoyan
author_facet Deng, Wusheng
Lei, Yanmei
Tang, Xiujia
Li, Dingbin
Liang, Jinhua
Luo, Jing
Liu, Liuyuan
Zhang, Wenshu
Ye, Liumei
Kong, Jinliang
Wang, Ke
Chen, Zhaoyan
author_sort Deng, Wusheng
collection PubMed
description Anti-infection strategies against pleural empyema include the use of antibiotics and drainage treatments, but bacterial eradication rates remain low. A major challenge is the formation of biofilms in the pleural cavity. DNase has antibiofilm efficacy in vitro, and intrapleural therapy with DNase is recommended to treat pleural empyema, but the relevant mechanisms remain limited. Our aim was to investigate whether DNase I inhibit the early biofilm formation in Pseudomonas aeruginosa- or Staphylococcus aureus-induced empyema models. We used various assays, such as crystal violet staining, confocal laser scanning microscopy (CLSM) analysis, peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH), and scanning electron microscopy (SEM) analysis. Our results suggested that DNase I significantly inhibited early biofilm formation in a dose-dependent manner, without affecting the growth of P. aeruginosa or S. aureus in vitro. CLSM analysis confirmed that DNase I decreased the biomass and thickness of both bacterial biofilms. The PNA-FISH and SEM analyses also revealed that DNase I inhibited early (24h) biofilm formation in two empyema models. Thus, the results indicated that DNase inhibited early (24h) biofilm formation in P. aeruginosa- or S. aureus-induced rabbit empyema models and showed its therapeutic potential against empyema biofilms.
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spelling pubmed-95976952022-10-27 DNase inhibits early biofilm formation in Pseudomonas aeruginosa- or Staphylococcus aureus-induced empyema models Deng, Wusheng Lei, Yanmei Tang, Xiujia Li, Dingbin Liang, Jinhua Luo, Jing Liu, Liuyuan Zhang, Wenshu Ye, Liumei Kong, Jinliang Wang, Ke Chen, Zhaoyan Front Cell Infect Microbiol Cellular and Infection Microbiology Anti-infection strategies against pleural empyema include the use of antibiotics and drainage treatments, but bacterial eradication rates remain low. A major challenge is the formation of biofilms in the pleural cavity. DNase has antibiofilm efficacy in vitro, and intrapleural therapy with DNase is recommended to treat pleural empyema, but the relevant mechanisms remain limited. Our aim was to investigate whether DNase I inhibit the early biofilm formation in Pseudomonas aeruginosa- or Staphylococcus aureus-induced empyema models. We used various assays, such as crystal violet staining, confocal laser scanning microscopy (CLSM) analysis, peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH), and scanning electron microscopy (SEM) analysis. Our results suggested that DNase I significantly inhibited early biofilm formation in a dose-dependent manner, without affecting the growth of P. aeruginosa or S. aureus in vitro. CLSM analysis confirmed that DNase I decreased the biomass and thickness of both bacterial biofilms. The PNA-FISH and SEM analyses also revealed that DNase I inhibited early (24h) biofilm formation in two empyema models. Thus, the results indicated that DNase inhibited early (24h) biofilm formation in P. aeruginosa- or S. aureus-induced rabbit empyema models and showed its therapeutic potential against empyema biofilms. Frontiers Media S.A. 2022-10-12 /pmc/articles/PMC9597695/ /pubmed/36310876 http://dx.doi.org/10.3389/fcimb.2022.917038 Text en Copyright © 2022 Deng, Lei, Tang, Li, Liang, Luo, Liu, Zhang, Ye, Kong, Wang and Chen https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Deng, Wusheng
Lei, Yanmei
Tang, Xiujia
Li, Dingbin
Liang, Jinhua
Luo, Jing
Liu, Liuyuan
Zhang, Wenshu
Ye, Liumei
Kong, Jinliang
Wang, Ke
Chen, Zhaoyan
DNase inhibits early biofilm formation in Pseudomonas aeruginosa- or Staphylococcus aureus-induced empyema models
title DNase inhibits early biofilm formation in Pseudomonas aeruginosa- or Staphylococcus aureus-induced empyema models
title_full DNase inhibits early biofilm formation in Pseudomonas aeruginosa- or Staphylococcus aureus-induced empyema models
title_fullStr DNase inhibits early biofilm formation in Pseudomonas aeruginosa- or Staphylococcus aureus-induced empyema models
title_full_unstemmed DNase inhibits early biofilm formation in Pseudomonas aeruginosa- or Staphylococcus aureus-induced empyema models
title_short DNase inhibits early biofilm formation in Pseudomonas aeruginosa- or Staphylococcus aureus-induced empyema models
title_sort dnase inhibits early biofilm formation in pseudomonas aeruginosa- or staphylococcus aureus-induced empyema models
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9597695/
https://www.ncbi.nlm.nih.gov/pubmed/36310876
http://dx.doi.org/10.3389/fcimb.2022.917038
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