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Occurrence of Borrelia sp. among Wild Living Invasive and Native Mesocarnivores in Poland

SIMPLE SUMMARY: Vertebrate hosts, especially wild living animals, are pivotal to the circulation and maintenance of Borrelia spp. Mesocarnivores are involved in Lyme disease ecology in sylvatic and suburban ecosystems. In this study, we aimed to examine the relative importance of six medium-sized ca...

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Autores principales: Hildebrand, Joanna, Jurczyk, Kacper, Popiołek, Marcin, Buńkowska-Gawlik, Katarzyna, Perec-Matysiak, Agnieszka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9597796/
https://www.ncbi.nlm.nih.gov/pubmed/36290214
http://dx.doi.org/10.3390/ani12202829
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author Hildebrand, Joanna
Jurczyk, Kacper
Popiołek, Marcin
Buńkowska-Gawlik, Katarzyna
Perec-Matysiak, Agnieszka
author_facet Hildebrand, Joanna
Jurczyk, Kacper
Popiołek, Marcin
Buńkowska-Gawlik, Katarzyna
Perec-Matysiak, Agnieszka
author_sort Hildebrand, Joanna
collection PubMed
description SIMPLE SUMMARY: Vertebrate hosts, especially wild living animals, are pivotal to the circulation and maintenance of Borrelia spp. Mesocarnivores are involved in Lyme disease ecology in sylvatic and suburban ecosystems. In this study, we aimed to examine the relative importance of six medium-sized carnivore species, raccoon, red fox, raccoon dog, European badger, pine marten and stone marten, as hosts of Borrelia spp. and investigated their role in this spirochaete’s transmission cycle. We also aimed to trace the reservoir competence of these invasive and native predators and the eco-epidemiology of Borrelia spp. in the context of a dilution effect. In all examined carnivore species, the occurrence of Borrelia was recorded, and the results suggest that raccoons may play a role as reservoir hosts for these spirochaetal bacteria. The role of invasive species seems to be worthy of further analysis with reference to the circulation of vector-borne pathogens as well as in the context of the “dilution effect” hypothesis. ABSTRACT: Wild living mesocarnivores, both introduced and native species, are able to adapt well to peri-urban environments, facilitating cross-species pathogen transmission with domestic animals, and potentially humans. Individual tissue samples derived from 284 specimens of six carnivore species, i.e., raccoon, raccoon dog, red fox, European badger, pine marten and stone marten, were used for molecular investigations with the nested PCR method. The animals were sampled in the Ruszów Forest District (Poland). We aimed to examine the relative importance of the studied mesocarnivores as hosts of Borrelia spp. and investigated their role in this spirochaete’s transmission cycle. We also aimed to trace the reservoir competence of these invasive and native predators and borreliosis eco-epidemiology in the context of a dilution effect. The overall prevalence of Borrelia spp. in the tested carnivores was 8.8%. Almost all of the consensus sequences of the partial flaB gene shared identity with a sequence of specific Borrelia species, i.e., B. afzelii, B. garinii and B. burgdorferi. Our results suggest that raccoons may play a role as reservoir hosts for these spirochaetal bacteria. The role of invasive species seems to be worthy of further analysis with reference to the circulation of vector-borne pathogens as well as in the context of the “dilution effect” hypothesis.
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spelling pubmed-95977962022-10-27 Occurrence of Borrelia sp. among Wild Living Invasive and Native Mesocarnivores in Poland Hildebrand, Joanna Jurczyk, Kacper Popiołek, Marcin Buńkowska-Gawlik, Katarzyna Perec-Matysiak, Agnieszka Animals (Basel) Article SIMPLE SUMMARY: Vertebrate hosts, especially wild living animals, are pivotal to the circulation and maintenance of Borrelia spp. Mesocarnivores are involved in Lyme disease ecology in sylvatic and suburban ecosystems. In this study, we aimed to examine the relative importance of six medium-sized carnivore species, raccoon, red fox, raccoon dog, European badger, pine marten and stone marten, as hosts of Borrelia spp. and investigated their role in this spirochaete’s transmission cycle. We also aimed to trace the reservoir competence of these invasive and native predators and the eco-epidemiology of Borrelia spp. in the context of a dilution effect. In all examined carnivore species, the occurrence of Borrelia was recorded, and the results suggest that raccoons may play a role as reservoir hosts for these spirochaetal bacteria. The role of invasive species seems to be worthy of further analysis with reference to the circulation of vector-borne pathogens as well as in the context of the “dilution effect” hypothesis. ABSTRACT: Wild living mesocarnivores, both introduced and native species, are able to adapt well to peri-urban environments, facilitating cross-species pathogen transmission with domestic animals, and potentially humans. Individual tissue samples derived from 284 specimens of six carnivore species, i.e., raccoon, raccoon dog, red fox, European badger, pine marten and stone marten, were used for molecular investigations with the nested PCR method. The animals were sampled in the Ruszów Forest District (Poland). We aimed to examine the relative importance of the studied mesocarnivores as hosts of Borrelia spp. and investigated their role in this spirochaete’s transmission cycle. We also aimed to trace the reservoir competence of these invasive and native predators and borreliosis eco-epidemiology in the context of a dilution effect. The overall prevalence of Borrelia spp. in the tested carnivores was 8.8%. Almost all of the consensus sequences of the partial flaB gene shared identity with a sequence of specific Borrelia species, i.e., B. afzelii, B. garinii and B. burgdorferi. Our results suggest that raccoons may play a role as reservoir hosts for these spirochaetal bacteria. The role of invasive species seems to be worthy of further analysis with reference to the circulation of vector-borne pathogens as well as in the context of the “dilution effect” hypothesis. MDPI 2022-10-18 /pmc/articles/PMC9597796/ /pubmed/36290214 http://dx.doi.org/10.3390/ani12202829 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hildebrand, Joanna
Jurczyk, Kacper
Popiołek, Marcin
Buńkowska-Gawlik, Katarzyna
Perec-Matysiak, Agnieszka
Occurrence of Borrelia sp. among Wild Living Invasive and Native Mesocarnivores in Poland
title Occurrence of Borrelia sp. among Wild Living Invasive and Native Mesocarnivores in Poland
title_full Occurrence of Borrelia sp. among Wild Living Invasive and Native Mesocarnivores in Poland
title_fullStr Occurrence of Borrelia sp. among Wild Living Invasive and Native Mesocarnivores in Poland
title_full_unstemmed Occurrence of Borrelia sp. among Wild Living Invasive and Native Mesocarnivores in Poland
title_short Occurrence of Borrelia sp. among Wild Living Invasive and Native Mesocarnivores in Poland
title_sort occurrence of borrelia sp. among wild living invasive and native mesocarnivores in poland
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9597796/
https://www.ncbi.nlm.nih.gov/pubmed/36290214
http://dx.doi.org/10.3390/ani12202829
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