Serum Analytes of American Mink (Neovison Vison) Challenged with Aleutian Mink Disease Virus

SIMPLE SUMMARY: Aleutian mink disease virus (AMDV) causes major health problems in the mink industry worldwide. The disease caused by AMDV has no cure or effective vaccine, and long-term viral eradication programs have failed in many countries. Some AMDV infected mink are genetically capable of tolerating the infection and living healthy and productive lives. Genetic selection for tolerance is, thus, a practical strategy to combat this virus. Accurate identification of tolerant animals is the fundamental issue in selection programs. The concentrations of some blood analytes, which are widely used as indicators of the presence and severity of diseases in humans and animals, are known to increase the accuracy of identifying tolerant mink. The objective of this study was to evaluate the merits of 14 serum analytes as biomarkers of tolerance to AMDV infection. Blood samples from 493 AMDV inoculated mink collected between 120 and 1211 days post-inoculation were analyzed. Total serum protein and globulin were found to be the most useful biomarkers of tolerance, whereas the relationships of other serum analytes to tolerance were weak or negligible. ABSTRACT: Black American mink (Neovison vison), which had been selected for tolerance to Aleutian mink disease virus (AMDV) for more than 20 years (TG100) or were from herds that have been free of AMDV (TG0), along with their progeny and crosses with 50% and 75% tolerance ancestry, were inoculated with a local isolate of AMDV. Blood samples were collected from 493 mink between 120 and 1211 days post-inoculation, and concentrations of 14 serum analytes were measured. Distributions of all analytes significantly deviated from normality, and data were analyzed after Box–Cox power transformation. Significant differences were observed among tolerant groups in the concentrations of globulin (GLO), total protein (TP), alkaline phosphatase, urea nitrogen, and calcium. Concentrations of GLO and TP linearly and significantly decreased with an increasing percentage of tolerance ancestry. Eleven analytes had the smallest values in the tolerant groups (TG100 or TG75), and eight analytes had the greatest values in the non-selected groups (TG0 or TG50). Antibody titer had the greatest correlation coefficients with GLO (0.62), TP (0.53), and creatinine (0.36). It was concluded that selection for tolerance decreased the concentrations of most serum analytes, and TP and GLO were the most accurate biomarkers of tolerance to AMDV infection. Males had significantly greater values than females for phosphorus and total bilirubin concentrations, but females had significantly greater amylase, cholesterol, and BUN concentrations than males.