Comparative Study of Pain-Related Responses of Male Piglets up to Seven Days of Age to the Application of Different Local Anaesthetics and Subsequent Castration

SIMPLE SUMMARY: Since 2021, surgical piglet castration must be performed with complete pain elimination according to the Animal Protection Law in Germany. General anaesthesia by isoflurane inhalation, which can be performed by the farmer, or by injection of ketamine and azaperone, which must be performed by a veterinarian, are the options available. At present, local anaesthesia is still under debate because of the lack of proof of complete pain elimination and the pain on injection. We tested three local anaesthetics (procaine, lidocaine, and mepivacaine) at two different doses each. Because pain responses can be masked by reactions caused by handling, the piglets were given superficial isoflurane anaesthesia. The pain on injection to the testes was compared with intramuscular injection, and the effectiveness during castration was compared among the local anaesthetics. Nocifensive movements, respiratory rate, blood pressure, heart rate and its variability as well as electroencephalography (EEG) changes were studied in relation to the painful interventions. Most indicators of nociception point to testicular injection pain being beyond intramuscular injection pain when an effective amount of local anaesthetic was used. However, complete pain elimination could not be achieved during castration under local anaesthesia. ABSTRACT: To evaluate pain responses to intratesticular and subscrotal injection of three local anaesthetics and their efficacy during castration a randomized controlled study was conducted. In groups of 20 piglets, procaine (2%), lidocaine (2%), or mepivacaine (2%) were administered subscrotal and intratesticularly in two different dosages: 0.5 mL of the original substances or the maximum recommended dosage according to body weight diluted with isotonic saline to a volume of 0.3 mL per each injection site. Two placebo groups received the equivalent volume of isotonic saline. A control group was injected intramuscularly with 0.5 mL isotonic saline for injection pain comparison. Electroencephalographic changes, respiratory rate, heart rate and its variability, blood pressure, and nocifensive movements were assessed in superficial isoflurane anaesthesia. While EEG-changes and linear measures of heart rate variability did not appear conclusive, the low frequency/high frequency (LF/HF) ratio corresponded best with the other pain indicators recorded. The injection of 0.3 mL diluted local anaesthetic per injection site elicited significant fewer signs of pain compared to intramuscular injection of saline. However, pain reduction, but not complete pain elimination, during castration could only be achieved with 0.5 mL of the 2% local anaesthetics per injection site, whereby lidocaine and mepivacaine were the most effective.