Ikhnos: A Novel Software to Register and Analyze Bone Surface Modifications Based on Three-Dimensional Documentation

SIMPLE SUMMARY: Here we present a novel open-access 3D software called Ikhnos to register and analyze bone surface modifications in bone assemblages using a sample of wild and captive wolf populations as an example to demonstrate the possibilities offered by this newly developed toolkit. The study of bone surface modifications has been proven crucial in understanding archaeological and paleontological site formation processes, including the identification of the assemblage causal agent. The use of the tools provided by Ikhnos will help interpret the fossil record by allowing the generation of accurate biological and physicochemical referential models as well as the precise documentation of bone surface modifications in archaeological and paleontological assemblages. ABSTRACT: The study of bone surface modifications (BSM) is crucial in understanding site formation processes and the identification of the causal agent behind bone assemblages in the fossil record. In that line, many efforts have been made to generate referential models based on feeding experiments and human butchery simulations that can then be used to interpret the patterns observed in archaeological and paleontological sites. Considering these needs, we developed a novel open-access three-dimensional (3D) software called Ikhnos for the study of BSM distribution patterns on limb long bones. This software is comprised of all the necessary tools for the 3D documentation of BSM and bone breakage patterns, as well as the subsequent statistical analysis of this data due to the integration of an exclusive R library, the IkhnosToolBox. Additionally, Ikhnos integrates tools for bone survivorship calculations that could facilitate the estimation of the minimum number of elements (MNE) and minimum number of individuals (MNI). As a demonstration of its precision, here we present a case study analyzing the modifications produced by wild and captive wolf (Canis lupus signatus) populations of the Iberian Peninsula on deer carcasses.