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Photosynthesis Monitoring in Microalgae Cultures Grown on Municipal Wastewater as a Nutrient Source in Large-Scale Outdoor Bioreactors
SIMPLE SUMMARY: The remediation of waste nutrients, mainly nitrogen, phosphorous, and carbon, results in low-cost biomass production. In this work, raceway ponds with an area of 1 and 0.5 ha were built in Mérida, Spain next to a municipal wastewater (WW) treatment plant. These DEMO bioreactors are o...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9598107/ https://www.ncbi.nlm.nih.gov/pubmed/36290287 http://dx.doi.org/10.3390/biology11101380 |
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author | Masojídek, Jiří Gómez-Serrano, Cintia Ranglová, Karolína Cicchi, Bernardo Encinas Bogeat, Ángel Câmara Manoel, João A. Sanches Zurano, Ana Silva Benavides, Ana Margarita Barceló-Villalobos, Marta Robles Carnero, Victor A. Ördög, Vince Gómez Pinchetti, Juan Luis Vörös, Lajos Arbib, Zouhayr Rogalla, Frank Torzillo, Giuseppe Lopez Figueroa, Félix Acién-Fernándéz, Francisco Gabriel |
author_facet | Masojídek, Jiří Gómez-Serrano, Cintia Ranglová, Karolína Cicchi, Bernardo Encinas Bogeat, Ángel Câmara Manoel, João A. Sanches Zurano, Ana Silva Benavides, Ana Margarita Barceló-Villalobos, Marta Robles Carnero, Victor A. Ördög, Vince Gómez Pinchetti, Juan Luis Vörös, Lajos Arbib, Zouhayr Rogalla, Frank Torzillo, Giuseppe Lopez Figueroa, Félix Acién-Fernándéz, Francisco Gabriel |
author_sort | Masojídek, Jiří |
collection | PubMed |
description | SIMPLE SUMMARY: The remediation of waste nutrients, mainly nitrogen, phosphorous, and carbon, results in low-cost biomass production. In this work, raceway ponds with an area of 1 and 0.5 ha were built in Mérida, Spain next to a municipal wastewater (WW) treatment plant. These DEMO bioreactors are operated continuously all year round. At the start of the trials, the bioreactors were filled with urban WW as a sole source of nutrients and left to be populated by the sewage-born phytoplankton. The fitness and growth of the phytoplankton population (dominated by the green microalga Micractinium) were monitored by photosynthesis measuring techniques. The cultures showed suitable photosynthetic activity. Productivity values ranged between 15 and 25 g DW m(−2) day(−1) and processed biomass revealed biostimulating activity. In these trials, photosynthesis measuring techniques, i.e., oxygen evolution and chlorophyll (Chl) fluorescence techniques, were validated to monitor large-scale bioreactors using municipal WW remediation for biomass production, which can be used for agricultural purposes as biofertilizer/biostimulant. ABSTRACT: Microalgae cultures were used for a WW treatment to remediate nutrients while producing biomass and recycling water. In these trials, raceway ponds (RWPs; 1 and 0.5 ha) were located next to a municipal (WW) treatment plant in Mérida, Spain. The ponds were used for continuous, all-year-round microalgae production using WW as a source of nutrients. Neither CO(2) nor air was supplied to cultures. The objective was to validate photosynthesis monitoring techniques in large-scale bioreactors. Various in-situ/ex-situ methods based on chlorophyll fluorescence and oxygen evolution measurements were used to follow culture performance. Photosynthesis variables gathered with these techniques were compared to the physiological behavior and growth of cultures. Good photosynthetic activity was indicated by the build-up of dissolved oxygen concentration up to 380% saturation, high photochemical yield (Fv/Fm = 0.62–0.71), and relative electron transport rate rETR between 200 and 450 μmol e(−) m(−2) s(−1) at midday, which resulted in biomass productivity of about 15–25 g DW m(−2) day(−1). The variables represent reliable markers reflecting the physiological status of microalgae cultures. Using waste nutrients, the biomass production cost can be significantly decreased for abundant biomass production in large-scale bioreactors, which can be exploited for agricultural purposes. |
format | Online Article Text |
id | pubmed-9598107 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-95981072022-10-27 Photosynthesis Monitoring in Microalgae Cultures Grown on Municipal Wastewater as a Nutrient Source in Large-Scale Outdoor Bioreactors Masojídek, Jiří Gómez-Serrano, Cintia Ranglová, Karolína Cicchi, Bernardo Encinas Bogeat, Ángel Câmara Manoel, João A. Sanches Zurano, Ana Silva Benavides, Ana Margarita Barceló-Villalobos, Marta Robles Carnero, Victor A. Ördög, Vince Gómez Pinchetti, Juan Luis Vörös, Lajos Arbib, Zouhayr Rogalla, Frank Torzillo, Giuseppe Lopez Figueroa, Félix Acién-Fernándéz, Francisco Gabriel Biology (Basel) Article SIMPLE SUMMARY: The remediation of waste nutrients, mainly nitrogen, phosphorous, and carbon, results in low-cost biomass production. In this work, raceway ponds with an area of 1 and 0.5 ha were built in Mérida, Spain next to a municipal wastewater (WW) treatment plant. These DEMO bioreactors are operated continuously all year round. At the start of the trials, the bioreactors were filled with urban WW as a sole source of nutrients and left to be populated by the sewage-born phytoplankton. The fitness and growth of the phytoplankton population (dominated by the green microalga Micractinium) were monitored by photosynthesis measuring techniques. The cultures showed suitable photosynthetic activity. Productivity values ranged between 15 and 25 g DW m(−2) day(−1) and processed biomass revealed biostimulating activity. In these trials, photosynthesis measuring techniques, i.e., oxygen evolution and chlorophyll (Chl) fluorescence techniques, were validated to monitor large-scale bioreactors using municipal WW remediation for biomass production, which can be used for agricultural purposes as biofertilizer/biostimulant. ABSTRACT: Microalgae cultures were used for a WW treatment to remediate nutrients while producing biomass and recycling water. In these trials, raceway ponds (RWPs; 1 and 0.5 ha) were located next to a municipal (WW) treatment plant in Mérida, Spain. The ponds were used for continuous, all-year-round microalgae production using WW as a source of nutrients. Neither CO(2) nor air was supplied to cultures. The objective was to validate photosynthesis monitoring techniques in large-scale bioreactors. Various in-situ/ex-situ methods based on chlorophyll fluorescence and oxygen evolution measurements were used to follow culture performance. Photosynthesis variables gathered with these techniques were compared to the physiological behavior and growth of cultures. Good photosynthetic activity was indicated by the build-up of dissolved oxygen concentration up to 380% saturation, high photochemical yield (Fv/Fm = 0.62–0.71), and relative electron transport rate rETR between 200 and 450 μmol e(−) m(−2) s(−1) at midday, which resulted in biomass productivity of about 15–25 g DW m(−2) day(−1). The variables represent reliable markers reflecting the physiological status of microalgae cultures. Using waste nutrients, the biomass production cost can be significantly decreased for abundant biomass production in large-scale bioreactors, which can be exploited for agricultural purposes. MDPI 2022-09-22 /pmc/articles/PMC9598107/ /pubmed/36290287 http://dx.doi.org/10.3390/biology11101380 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Masojídek, Jiří Gómez-Serrano, Cintia Ranglová, Karolína Cicchi, Bernardo Encinas Bogeat, Ángel Câmara Manoel, João A. Sanches Zurano, Ana Silva Benavides, Ana Margarita Barceló-Villalobos, Marta Robles Carnero, Victor A. Ördög, Vince Gómez Pinchetti, Juan Luis Vörös, Lajos Arbib, Zouhayr Rogalla, Frank Torzillo, Giuseppe Lopez Figueroa, Félix Acién-Fernándéz, Francisco Gabriel Photosynthesis Monitoring in Microalgae Cultures Grown on Municipal Wastewater as a Nutrient Source in Large-Scale Outdoor Bioreactors |
title | Photosynthesis Monitoring in Microalgae Cultures Grown on Municipal Wastewater as a Nutrient Source in Large-Scale Outdoor Bioreactors |
title_full | Photosynthesis Monitoring in Microalgae Cultures Grown on Municipal Wastewater as a Nutrient Source in Large-Scale Outdoor Bioreactors |
title_fullStr | Photosynthesis Monitoring in Microalgae Cultures Grown on Municipal Wastewater as a Nutrient Source in Large-Scale Outdoor Bioreactors |
title_full_unstemmed | Photosynthesis Monitoring in Microalgae Cultures Grown on Municipal Wastewater as a Nutrient Source in Large-Scale Outdoor Bioreactors |
title_short | Photosynthesis Monitoring in Microalgae Cultures Grown on Municipal Wastewater as a Nutrient Source in Large-Scale Outdoor Bioreactors |
title_sort | photosynthesis monitoring in microalgae cultures grown on municipal wastewater as a nutrient source in large-scale outdoor bioreactors |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9598107/ https://www.ncbi.nlm.nih.gov/pubmed/36290287 http://dx.doi.org/10.3390/biology11101380 |
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