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¹H-NMR Metabolic Profiling, Antioxidant Activity, and Docking Study of Common Medicinal Plant-Derived Honey

The purpose of this investigation was to determine ¹H-NMR profiling and antioxidant activity of the most common types of honey, namely, citrus honey (HC1) (Morcott tangerine L. and Jaffa orange L.), marjoram honey (HM1) (Origanum majorana L.), and clover honey (HT1) (Trifolium alexandrinum L.), comp...

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Autores principales: Montaser, Maha, Ali, Asmaa T., Sayed, Ahmed M., Abdelmohsen, Usama Ramadan, Zidan, Ehab W., Orfali, Raha, Rateb, Mostafa E., Zaki, Mohamed A., Hassan, Hossam M., Mohammed, Rabab, Hifnawy, Mohamed S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9598149/
https://www.ncbi.nlm.nih.gov/pubmed/36290603
http://dx.doi.org/10.3390/antiox11101880
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author Montaser, Maha
Ali, Asmaa T.
Sayed, Ahmed M.
Abdelmohsen, Usama Ramadan
Zidan, Ehab W.
Orfali, Raha
Rateb, Mostafa E.
Zaki, Mohamed A.
Hassan, Hossam M.
Mohammed, Rabab
Hifnawy, Mohamed S.
author_facet Montaser, Maha
Ali, Asmaa T.
Sayed, Ahmed M.
Abdelmohsen, Usama Ramadan
Zidan, Ehab W.
Orfali, Raha
Rateb, Mostafa E.
Zaki, Mohamed A.
Hassan, Hossam M.
Mohammed, Rabab
Hifnawy, Mohamed S.
author_sort Montaser, Maha
collection PubMed
description The purpose of this investigation was to determine ¹H-NMR profiling and antioxidant activity of the most common types of honey, namely, citrus honey (HC1) (Morcott tangerine L. and Jaffa orange L.), marjoram honey (HM1) (Origanum majorana L.), and clover honey (HT1) (Trifolium alexandrinum L.), compared to their secondary metabolites (HC2, HM2, HT2, respectively). By using a ¹H-NMR-based metabolomic technique, PCA, and PLS-DA multivariate analysis, we found that HC2, HM2, HC1, and HM1 were clustered together. However, HT1 and HT2 were quite far from these and each other. This indicated that HC1, HM1, HC2, and HM2 have similar chemical compositions, while HT1 and HT2 were unique in their chemical profiles. Antioxidation potentials were determined colorimetrically for scavenging activities against DPPH, ABTS, ORAC, 5-LOX, and metal chelating activity in all honey extract samples and their secondary metabolites. Our results revealed that HC2 and HM2 possessed more antioxidant activities than HT2 in vitro. HC2 demonstrated the highest antioxidant effect in all assays, followed by HM2 (DPPH assay: IC(50) 2.91, 10.7 μg/mL; ABTS assay: 431.2, 210.24 at 50 ug/mL Trolox equivalent; ORAC assay: 259.5, 234.8 at 50 ug/mL Trolox equivalent; 5-LOX screening assay/IC(50): 2.293, 6.136 ug/mL; and metal chelating activity at 50 ug/mL: 73.34526%, 63.75881% inhibition). We suggest that the presence of some secondary metabolites in HC and HM, such as hesperetin, linalool, and caffeic acid, increased the antioxidant activity in citrus and marjoram compared to clover honey.
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spelling pubmed-95981492022-10-27 ¹H-NMR Metabolic Profiling, Antioxidant Activity, and Docking Study of Common Medicinal Plant-Derived Honey Montaser, Maha Ali, Asmaa T. Sayed, Ahmed M. Abdelmohsen, Usama Ramadan Zidan, Ehab W. Orfali, Raha Rateb, Mostafa E. Zaki, Mohamed A. Hassan, Hossam M. Mohammed, Rabab Hifnawy, Mohamed S. Antioxidants (Basel) Article The purpose of this investigation was to determine ¹H-NMR profiling and antioxidant activity of the most common types of honey, namely, citrus honey (HC1) (Morcott tangerine L. and Jaffa orange L.), marjoram honey (HM1) (Origanum majorana L.), and clover honey (HT1) (Trifolium alexandrinum L.), compared to their secondary metabolites (HC2, HM2, HT2, respectively). By using a ¹H-NMR-based metabolomic technique, PCA, and PLS-DA multivariate analysis, we found that HC2, HM2, HC1, and HM1 were clustered together. However, HT1 and HT2 were quite far from these and each other. This indicated that HC1, HM1, HC2, and HM2 have similar chemical compositions, while HT1 and HT2 were unique in their chemical profiles. Antioxidation potentials were determined colorimetrically for scavenging activities against DPPH, ABTS, ORAC, 5-LOX, and metal chelating activity in all honey extract samples and their secondary metabolites. Our results revealed that HC2 and HM2 possessed more antioxidant activities than HT2 in vitro. HC2 demonstrated the highest antioxidant effect in all assays, followed by HM2 (DPPH assay: IC(50) 2.91, 10.7 μg/mL; ABTS assay: 431.2, 210.24 at 50 ug/mL Trolox equivalent; ORAC assay: 259.5, 234.8 at 50 ug/mL Trolox equivalent; 5-LOX screening assay/IC(50): 2.293, 6.136 ug/mL; and metal chelating activity at 50 ug/mL: 73.34526%, 63.75881% inhibition). We suggest that the presence of some secondary metabolites in HC and HM, such as hesperetin, linalool, and caffeic acid, increased the antioxidant activity in citrus and marjoram compared to clover honey. MDPI 2022-09-22 /pmc/articles/PMC9598149/ /pubmed/36290603 http://dx.doi.org/10.3390/antiox11101880 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Montaser, Maha
Ali, Asmaa T.
Sayed, Ahmed M.
Abdelmohsen, Usama Ramadan
Zidan, Ehab W.
Orfali, Raha
Rateb, Mostafa E.
Zaki, Mohamed A.
Hassan, Hossam M.
Mohammed, Rabab
Hifnawy, Mohamed S.
¹H-NMR Metabolic Profiling, Antioxidant Activity, and Docking Study of Common Medicinal Plant-Derived Honey
title ¹H-NMR Metabolic Profiling, Antioxidant Activity, and Docking Study of Common Medicinal Plant-Derived Honey
title_full ¹H-NMR Metabolic Profiling, Antioxidant Activity, and Docking Study of Common Medicinal Plant-Derived Honey
title_fullStr ¹H-NMR Metabolic Profiling, Antioxidant Activity, and Docking Study of Common Medicinal Plant-Derived Honey
title_full_unstemmed ¹H-NMR Metabolic Profiling, Antioxidant Activity, and Docking Study of Common Medicinal Plant-Derived Honey
title_short ¹H-NMR Metabolic Profiling, Antioxidant Activity, and Docking Study of Common Medicinal Plant-Derived Honey
title_sort ¹h-nmr metabolic profiling, antioxidant activity, and docking study of common medicinal plant-derived honey
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9598149/
https://www.ncbi.nlm.nih.gov/pubmed/36290603
http://dx.doi.org/10.3390/antiox11101880
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