Cargando…
Bioinspired Lipase Immobilized Membrane for Improving Hesperidin Lipophilization
Lipophilization is a promising way to improve the bioavailability of flavonoids. However, the traditional enzymatic esterification methods are time-consuming, and present low yields and purity. Herein, a novel membrane-based lipophilization technology—bioinspired lipase immobilized membranes (BLIMs)...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9598331/ https://www.ncbi.nlm.nih.gov/pubmed/36290627 http://dx.doi.org/10.3390/antiox11101906 |
_version_ | 1784816307167821824 |
---|---|
author | Ming, Shanxiu Li, Shuyi Chen, Zhe Chen, Xujun Wang, Feifei Deng, Shaonan Marszałek, Krystian Zhu, Zhenzhou Zhang, Wenxiang Barba, Francisco J. |
author_facet | Ming, Shanxiu Li, Shuyi Chen, Zhe Chen, Xujun Wang, Feifei Deng, Shaonan Marszałek, Krystian Zhu, Zhenzhou Zhang, Wenxiang Barba, Francisco J. |
author_sort | Ming, Shanxiu |
collection | PubMed |
description | Lipophilization is a promising way to improve the bioavailability of flavonoids. However, the traditional enzymatic esterification methods are time-consuming, and present low yields and purity. Herein, a novel membrane-based lipophilization technology—bioinspired lipase immobilized membranes (BLIMs), including CAL-B@PES, CAL-B@PDA/PES and GA/CAL-B@PDA/PES— were fabricated to improve the antioxidant flavanone glycoside hesperidin lipophilization. Via reverse filtration, PDA coating and GA crosslinking, Candida antarctica lipase B (CAL-B) was stably immobilized on membrane to fabricate BLIMs. Among the three BLIMs, GA/CAL-B@PDA/PES had the greatest enzyme activity and enzyme loading, the strongest tolerance of changes in external environmental conditions (temperatures, pH, heating time, storage time and numbers of cycles) and the highest hesperidin esterification efficiency. Moreover, the optimal operating condition for GA/CAL-B@PDA/PES fabrication was the CAL-B concentration of 0.36 mg/mL, operation pressure of 2 bar, GA concentration of 5% and crosslinking time of 1 h. Afterwards, the hesperidin esterification process did not affect the micromorphology of BLIM, but clearly improved the BLIM permeability and esterified product efficiency. The present study reveals the fabrication mechanism of BLIMs and offers insights into the optimizing strategy that governs the membrane-based lipophilization technology process. |
format | Online Article Text |
id | pubmed-9598331 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-95983312022-10-27 Bioinspired Lipase Immobilized Membrane for Improving Hesperidin Lipophilization Ming, Shanxiu Li, Shuyi Chen, Zhe Chen, Xujun Wang, Feifei Deng, Shaonan Marszałek, Krystian Zhu, Zhenzhou Zhang, Wenxiang Barba, Francisco J. Antioxidants (Basel) Article Lipophilization is a promising way to improve the bioavailability of flavonoids. However, the traditional enzymatic esterification methods are time-consuming, and present low yields and purity. Herein, a novel membrane-based lipophilization technology—bioinspired lipase immobilized membranes (BLIMs), including CAL-B@PES, CAL-B@PDA/PES and GA/CAL-B@PDA/PES— were fabricated to improve the antioxidant flavanone glycoside hesperidin lipophilization. Via reverse filtration, PDA coating and GA crosslinking, Candida antarctica lipase B (CAL-B) was stably immobilized on membrane to fabricate BLIMs. Among the three BLIMs, GA/CAL-B@PDA/PES had the greatest enzyme activity and enzyme loading, the strongest tolerance of changes in external environmental conditions (temperatures, pH, heating time, storage time and numbers of cycles) and the highest hesperidin esterification efficiency. Moreover, the optimal operating condition for GA/CAL-B@PDA/PES fabrication was the CAL-B concentration of 0.36 mg/mL, operation pressure of 2 bar, GA concentration of 5% and crosslinking time of 1 h. Afterwards, the hesperidin esterification process did not affect the micromorphology of BLIM, but clearly improved the BLIM permeability and esterified product efficiency. The present study reveals the fabrication mechanism of BLIMs and offers insights into the optimizing strategy that governs the membrane-based lipophilization technology process. MDPI 2022-09-26 /pmc/articles/PMC9598331/ /pubmed/36290627 http://dx.doi.org/10.3390/antiox11101906 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ming, Shanxiu Li, Shuyi Chen, Zhe Chen, Xujun Wang, Feifei Deng, Shaonan Marszałek, Krystian Zhu, Zhenzhou Zhang, Wenxiang Barba, Francisco J. Bioinspired Lipase Immobilized Membrane for Improving Hesperidin Lipophilization |
title | Bioinspired Lipase Immobilized Membrane for Improving Hesperidin Lipophilization |
title_full | Bioinspired Lipase Immobilized Membrane for Improving Hesperidin Lipophilization |
title_fullStr | Bioinspired Lipase Immobilized Membrane for Improving Hesperidin Lipophilization |
title_full_unstemmed | Bioinspired Lipase Immobilized Membrane for Improving Hesperidin Lipophilization |
title_short | Bioinspired Lipase Immobilized Membrane for Improving Hesperidin Lipophilization |
title_sort | bioinspired lipase immobilized membrane for improving hesperidin lipophilization |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9598331/ https://www.ncbi.nlm.nih.gov/pubmed/36290627 http://dx.doi.org/10.3390/antiox11101906 |
work_keys_str_mv | AT mingshanxiu bioinspiredlipaseimmobilizedmembraneforimprovinghesperidinlipophilization AT lishuyi bioinspiredlipaseimmobilizedmembraneforimprovinghesperidinlipophilization AT chenzhe bioinspiredlipaseimmobilizedmembraneforimprovinghesperidinlipophilization AT chenxujun bioinspiredlipaseimmobilizedmembraneforimprovinghesperidinlipophilization AT wangfeifei bioinspiredlipaseimmobilizedmembraneforimprovinghesperidinlipophilization AT dengshaonan bioinspiredlipaseimmobilizedmembraneforimprovinghesperidinlipophilization AT marszałekkrystian bioinspiredlipaseimmobilizedmembraneforimprovinghesperidinlipophilization AT zhuzhenzhou bioinspiredlipaseimmobilizedmembraneforimprovinghesperidinlipophilization AT zhangwenxiang bioinspiredlipaseimmobilizedmembraneforimprovinghesperidinlipophilization AT barbafranciscoj bioinspiredlipaseimmobilizedmembraneforimprovinghesperidinlipophilization |