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Comparative Evaluation of Various Extraction Techniques for Secondary Metabolites from Bombax ceiba L. Flowering Plants along with In Vitro Anti-Diabetic Performance

Bombax ceiba L. (Family: Malvaceae) was rightly called the “silent doctor” in the past as every part of it had medicinal value. For centuries, humans have used this plant according to the traditional medicinal systems of China, Ayurveda, and tribal communities. Recently, with an emerging interest in...

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Autores principales: Yasien, Sara, Iqbal, Muhammad Muntazir, Javed, Mohsin, Alnuwaiser, Maha Abdallah, Iqbal, Shahid, Mahmood, Qaiser, Elkaeed, Eslam B., Dera, Ayed A., Alrbyawi, Hamad, Pashameah, Rami Adel, Alzahrani, Eman, Farouk, Abd-ElAziem
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9598353/
https://www.ncbi.nlm.nih.gov/pubmed/36290454
http://dx.doi.org/10.3390/bioengineering9100486
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author Yasien, Sara
Iqbal, Muhammad Muntazir
Javed, Mohsin
Alnuwaiser, Maha Abdallah
Iqbal, Shahid
Mahmood, Qaiser
Elkaeed, Eslam B.
Dera, Ayed A.
Alrbyawi, Hamad
Pashameah, Rami Adel
Alzahrani, Eman
Farouk, Abd-ElAziem
author_facet Yasien, Sara
Iqbal, Muhammad Muntazir
Javed, Mohsin
Alnuwaiser, Maha Abdallah
Iqbal, Shahid
Mahmood, Qaiser
Elkaeed, Eslam B.
Dera, Ayed A.
Alrbyawi, Hamad
Pashameah, Rami Adel
Alzahrani, Eman
Farouk, Abd-ElAziem
author_sort Yasien, Sara
collection PubMed
description Bombax ceiba L. (Family: Malvaceae) was rightly called the “silent doctor” in the past as every part of it had medicinal value. For centuries, humans have used this plant according to the traditional medicinal systems of China, Ayurveda, and tribal communities. Recently, with an emerging interest in herbals, attention has been paid to scientifically validating medicinal claims for the treatment of diabetes using secondary metabolites of B. ceiba L. flowers. In the present study, specific secondary metabolites from the flowers of B. ceiba L. were isolated in good yield using the solvent extraction methodology, and their in vitro anti-diabetic efficacy was examined. Extraction efficiency of each solvent for secondary metabolites was found in following order: water > ethanol> methanol > chloroform > petroleum ether. Quantitative analysis of secondary metabolites showed 120.33 ± 2.33 mg/gm polyphenols, 60.77 ± 1.02 mg/g flavonoids, 60.26 ± 1.20 mg/g glycosaponins, 0.167 ± 0.02 mg/g polysaccharides for water extract; 91.00 ± 1.00 mg/g polyphenols, 9.22 ± 1.02 mg/g flavonoids, 43.90 ± 0.30 mg/g glycosaponins, 0.090 ± 0.03 mg/g poly saccharides for ethanol extract; 52.00 ± 2.64 mg/g polyphenols, 35.22 ± 0.38 mg/g flavonoids, 72.26 ± 1.05 mg/g glycosaponins, 0.147 ± 0.01 mg/g polysaccharides for methanol extract; 11.33 ± 0.58 mg/g polyphenols, 23.66 ± 1.76 mg/g flavonoids, 32.8 ± 0.75 mg/g glycosaponins, 0.013 ± 0.02 mg/g polysaccharides for chloroform extract; and 3.33 ± 1.53 mg/g polyphenols, 1.89 ± 1.39 mg/g flavonoids, 21.67 ± 1.24 mg/g glycosaponins, 0.005 ± 0.01 mg/g polysaccharides for petroleum ether extract. Glucose uptake by yeast cells increased 70.38 ± 2.17% by water extract.
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spelling pubmed-95983532022-10-27 Comparative Evaluation of Various Extraction Techniques for Secondary Metabolites from Bombax ceiba L. Flowering Plants along with In Vitro Anti-Diabetic Performance Yasien, Sara Iqbal, Muhammad Muntazir Javed, Mohsin Alnuwaiser, Maha Abdallah Iqbal, Shahid Mahmood, Qaiser Elkaeed, Eslam B. Dera, Ayed A. Alrbyawi, Hamad Pashameah, Rami Adel Alzahrani, Eman Farouk, Abd-ElAziem Bioengineering (Basel) Article Bombax ceiba L. (Family: Malvaceae) was rightly called the “silent doctor” in the past as every part of it had medicinal value. For centuries, humans have used this plant according to the traditional medicinal systems of China, Ayurveda, and tribal communities. Recently, with an emerging interest in herbals, attention has been paid to scientifically validating medicinal claims for the treatment of diabetes using secondary metabolites of B. ceiba L. flowers. In the present study, specific secondary metabolites from the flowers of B. ceiba L. were isolated in good yield using the solvent extraction methodology, and their in vitro anti-diabetic efficacy was examined. Extraction efficiency of each solvent for secondary metabolites was found in following order: water > ethanol> methanol > chloroform > petroleum ether. Quantitative analysis of secondary metabolites showed 120.33 ± 2.33 mg/gm polyphenols, 60.77 ± 1.02 mg/g flavonoids, 60.26 ± 1.20 mg/g glycosaponins, 0.167 ± 0.02 mg/g polysaccharides for water extract; 91.00 ± 1.00 mg/g polyphenols, 9.22 ± 1.02 mg/g flavonoids, 43.90 ± 0.30 mg/g glycosaponins, 0.090 ± 0.03 mg/g poly saccharides for ethanol extract; 52.00 ± 2.64 mg/g polyphenols, 35.22 ± 0.38 mg/g flavonoids, 72.26 ± 1.05 mg/g glycosaponins, 0.147 ± 0.01 mg/g polysaccharides for methanol extract; 11.33 ± 0.58 mg/g polyphenols, 23.66 ± 1.76 mg/g flavonoids, 32.8 ± 0.75 mg/g glycosaponins, 0.013 ± 0.02 mg/g polysaccharides for chloroform extract; and 3.33 ± 1.53 mg/g polyphenols, 1.89 ± 1.39 mg/g flavonoids, 21.67 ± 1.24 mg/g glycosaponins, 0.005 ± 0.01 mg/g polysaccharides for petroleum ether extract. Glucose uptake by yeast cells increased 70.38 ± 2.17% by water extract. MDPI 2022-09-20 /pmc/articles/PMC9598353/ /pubmed/36290454 http://dx.doi.org/10.3390/bioengineering9100486 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yasien, Sara
Iqbal, Muhammad Muntazir
Javed, Mohsin
Alnuwaiser, Maha Abdallah
Iqbal, Shahid
Mahmood, Qaiser
Elkaeed, Eslam B.
Dera, Ayed A.
Alrbyawi, Hamad
Pashameah, Rami Adel
Alzahrani, Eman
Farouk, Abd-ElAziem
Comparative Evaluation of Various Extraction Techniques for Secondary Metabolites from Bombax ceiba L. Flowering Plants along with In Vitro Anti-Diabetic Performance
title Comparative Evaluation of Various Extraction Techniques for Secondary Metabolites from Bombax ceiba L. Flowering Plants along with In Vitro Anti-Diabetic Performance
title_full Comparative Evaluation of Various Extraction Techniques for Secondary Metabolites from Bombax ceiba L. Flowering Plants along with In Vitro Anti-Diabetic Performance
title_fullStr Comparative Evaluation of Various Extraction Techniques for Secondary Metabolites from Bombax ceiba L. Flowering Plants along with In Vitro Anti-Diabetic Performance
title_full_unstemmed Comparative Evaluation of Various Extraction Techniques for Secondary Metabolites from Bombax ceiba L. Flowering Plants along with In Vitro Anti-Diabetic Performance
title_short Comparative Evaluation of Various Extraction Techniques for Secondary Metabolites from Bombax ceiba L. Flowering Plants along with In Vitro Anti-Diabetic Performance
title_sort comparative evaluation of various extraction techniques for secondary metabolites from bombax ceiba l. flowering plants along with in vitro anti-diabetic performance
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9598353/
https://www.ncbi.nlm.nih.gov/pubmed/36290454
http://dx.doi.org/10.3390/bioengineering9100486
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