Cargando…
Ultrasonic Enhancement of Aqueous Two-Phase Extraction and Acid Hydrolysis of Flavonoids from Malvaviscus arboreus Cav. Flower for Evaluation of Antioxidant Activity
The ultrasonic-assisted aqueous two-phase extraction (UAATPE) of flavonoid glycosides from Malvaviscus arboreous Cav. flower (MACF) was developed using ethanol/ammonia sulfate systems, followed by the ultrasonic-assisted acid hydrolysis (UAAH) of the top extract with HCl solution. The optimization o...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9598477/ https://www.ncbi.nlm.nih.gov/pubmed/36290762 http://dx.doi.org/10.3390/antiox11102039 |
_version_ | 1784816343778852864 |
---|---|
author | Yuan, Tiefeng Huang, Jilong Gan, Lin Chen, Linzhou Zhong, Jinjian Liu, Zhaohan Wang, Liping Fan, Huajun |
author_facet | Yuan, Tiefeng Huang, Jilong Gan, Lin Chen, Linzhou Zhong, Jinjian Liu, Zhaohan Wang, Liping Fan, Huajun |
author_sort | Yuan, Tiefeng |
collection | PubMed |
description | The ultrasonic-assisted aqueous two-phase extraction (UAATPE) of flavonoid glycosides from Malvaviscus arboreous Cav. flower (MACF) was developed using ethanol/ammonia sulfate systems, followed by the ultrasonic-assisted acid hydrolysis (UAAH) of the top extract with HCl solution. The optimization of UAATPE and UAAH processes was accomplished by single-factor experiments and response surface methodology. As a result, the flavonoid glycosides enriched in the top phase could achieve a maximum yield of 35.9 ± 1.1 mg/g by UAATPE and were completely hydrolyzed by UAAH deglycosylation. The flavonoid glycosides and their hydrolyzates were separated and characterized by high-performance liquid chromatography and ultra-high-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry. Ultrasonic enhancement of the extraction and hydrolysis was explored by comparative study. Furthermore, the in vitro activity of the flavonoid glycosides and the aglycones were comprehensively evaluated by antioxidant activity assays, including ferric-reducing antioxidant power and scavenging DPPH, hydroxyl, and superoxide radicals. All of the IC(50) values suggest that the antioxidant activity of flavonoid aglycones was stronger than that of their glucosides and even vitamin C, revealing that the deglycosylated flavonoids from MACF were the more powerful antioxidants. This study provided an effective and eco-friendly strategy for the extraction, separation, and purification of flavonoids from MACF, as well as for the development of the potential flavonoid antioxidants. |
format | Online Article Text |
id | pubmed-9598477 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-95984772022-10-27 Ultrasonic Enhancement of Aqueous Two-Phase Extraction and Acid Hydrolysis of Flavonoids from Malvaviscus arboreus Cav. Flower for Evaluation of Antioxidant Activity Yuan, Tiefeng Huang, Jilong Gan, Lin Chen, Linzhou Zhong, Jinjian Liu, Zhaohan Wang, Liping Fan, Huajun Antioxidants (Basel) Article The ultrasonic-assisted aqueous two-phase extraction (UAATPE) of flavonoid glycosides from Malvaviscus arboreous Cav. flower (MACF) was developed using ethanol/ammonia sulfate systems, followed by the ultrasonic-assisted acid hydrolysis (UAAH) of the top extract with HCl solution. The optimization of UAATPE and UAAH processes was accomplished by single-factor experiments and response surface methodology. As a result, the flavonoid glycosides enriched in the top phase could achieve a maximum yield of 35.9 ± 1.1 mg/g by UAATPE and were completely hydrolyzed by UAAH deglycosylation. The flavonoid glycosides and their hydrolyzates were separated and characterized by high-performance liquid chromatography and ultra-high-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry. Ultrasonic enhancement of the extraction and hydrolysis was explored by comparative study. Furthermore, the in vitro activity of the flavonoid glycosides and the aglycones were comprehensively evaluated by antioxidant activity assays, including ferric-reducing antioxidant power and scavenging DPPH, hydroxyl, and superoxide radicals. All of the IC(50) values suggest that the antioxidant activity of flavonoid aglycones was stronger than that of their glucosides and even vitamin C, revealing that the deglycosylated flavonoids from MACF were the more powerful antioxidants. This study provided an effective and eco-friendly strategy for the extraction, separation, and purification of flavonoids from MACF, as well as for the development of the potential flavonoid antioxidants. MDPI 2022-10-16 /pmc/articles/PMC9598477/ /pubmed/36290762 http://dx.doi.org/10.3390/antiox11102039 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yuan, Tiefeng Huang, Jilong Gan, Lin Chen, Linzhou Zhong, Jinjian Liu, Zhaohan Wang, Liping Fan, Huajun Ultrasonic Enhancement of Aqueous Two-Phase Extraction and Acid Hydrolysis of Flavonoids from Malvaviscus arboreus Cav. Flower for Evaluation of Antioxidant Activity |
title | Ultrasonic Enhancement of Aqueous Two-Phase Extraction and Acid Hydrolysis of Flavonoids from Malvaviscus arboreus Cav. Flower for Evaluation of Antioxidant Activity |
title_full | Ultrasonic Enhancement of Aqueous Two-Phase Extraction and Acid Hydrolysis of Flavonoids from Malvaviscus arboreus Cav. Flower for Evaluation of Antioxidant Activity |
title_fullStr | Ultrasonic Enhancement of Aqueous Two-Phase Extraction and Acid Hydrolysis of Flavonoids from Malvaviscus arboreus Cav. Flower for Evaluation of Antioxidant Activity |
title_full_unstemmed | Ultrasonic Enhancement of Aqueous Two-Phase Extraction and Acid Hydrolysis of Flavonoids from Malvaviscus arboreus Cav. Flower for Evaluation of Antioxidant Activity |
title_short | Ultrasonic Enhancement of Aqueous Two-Phase Extraction and Acid Hydrolysis of Flavonoids from Malvaviscus arboreus Cav. Flower for Evaluation of Antioxidant Activity |
title_sort | ultrasonic enhancement of aqueous two-phase extraction and acid hydrolysis of flavonoids from malvaviscus arboreus cav. flower for evaluation of antioxidant activity |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9598477/ https://www.ncbi.nlm.nih.gov/pubmed/36290762 http://dx.doi.org/10.3390/antiox11102039 |
work_keys_str_mv | AT yuantiefeng ultrasonicenhancementofaqueoustwophaseextractionandacidhydrolysisofflavonoidsfrommalvaviscusarboreuscavflowerforevaluationofantioxidantactivity AT huangjilong ultrasonicenhancementofaqueoustwophaseextractionandacidhydrolysisofflavonoidsfrommalvaviscusarboreuscavflowerforevaluationofantioxidantactivity AT ganlin ultrasonicenhancementofaqueoustwophaseextractionandacidhydrolysisofflavonoidsfrommalvaviscusarboreuscavflowerforevaluationofantioxidantactivity AT chenlinzhou ultrasonicenhancementofaqueoustwophaseextractionandacidhydrolysisofflavonoidsfrommalvaviscusarboreuscavflowerforevaluationofantioxidantactivity AT zhongjinjian ultrasonicenhancementofaqueoustwophaseextractionandacidhydrolysisofflavonoidsfrommalvaviscusarboreuscavflowerforevaluationofantioxidantactivity AT liuzhaohan ultrasonicenhancementofaqueoustwophaseextractionandacidhydrolysisofflavonoidsfrommalvaviscusarboreuscavflowerforevaluationofantioxidantactivity AT wangliping ultrasonicenhancementofaqueoustwophaseextractionandacidhydrolysisofflavonoidsfrommalvaviscusarboreuscavflowerforevaluationofantioxidantactivity AT fanhuajun ultrasonicenhancementofaqueoustwophaseextractionandacidhydrolysisofflavonoidsfrommalvaviscusarboreuscavflowerforevaluationofantioxidantactivity |