Condensed and Hydrolyzable Tannins for Reducing Methane and Nitrous Oxide Emissions in Dairy Manure—A Laboratory Incubation Study †

SIMPLE SUMMARY: This study investigated the mitigation effect of different tannins on greenhouse gas emissions (GHG) from freshly collected dairy cattle manure. Quebracho and chestnut industrial tannin extracts were tested against a fresh cattle manure slurry. The results showed that all tannins added to freshly collected dairy manure could decrease N(2)O and CH(4) emissions over a 14-d incubation period. ABSTRACT: The objectives of this study were to (1) examine the effects of plant condensed (CT) and hydrolyzable tannin (HT) extracts on CH(4) and N(2)O emissions; (2) identify the reactions responsible for manure-derived GHG emissions, and (3) examine accompanying microbial community changes in fresh dairy manure. Five treatments were applied in triplicate to the freshly collected dairy manure, including 4% CT, 8% CT, 4% HT, 8% HT (V/V), and control (no tannin addition). Fresh dairy manure was placed into 710 mL glass incubation chambers. In vitro composted dairy manure samples were collected at 0, 24, 48, and 336 h after the start of incubation. Fluxes of N(2)O and CH(4) were measured for 5-min/h for 14 d at a constant ambient incubation temperature of 39 °C. The addition of quebracho CT significantly decreased the CH(4) flux rates compared to the tannin-free controls (215.9 mg/m(2)/h), with peaks of 75.6 and 89.6 mg/m(2)/h for 4 and 8% CT inclusion rates, respectively. Furthermore, CT significantly reduced cumulative CH(4) emission by 68.2 and 57.3% at 4 and 8% CT addition, respectively. The HT treatments failed to affect CH(4) reduction. However, both CT and HT reduced (p < 0.001) cumulative and flux rates of N(2)O emissions. The decrease in CH(4) flux with CT was associated with a reduction in the abundance of Bacteroidetes and Proteobacteria.