Cargando…

Antibiofilm Effect of Cinnamaldehyde-Chitosan Nanoparticles against the Biofilm of Staphylococcus aureus

Food contamination caused by food-spoilage bacteria and pathogenic bacteria seriously affects public health. Staphylococcus aureus is a typical foodborne pathogen which easily forms biofilm. Once biofilm is formed, it is difficult to remove. The use of nanotechnology for antibiofilm purposes is beco...

Descripción completa

Detalles Bibliográficos
Autores principales: Xu, Jiaman, Lin, Quan, Sheng, Maokun, Ding, Ting, Li, Bing, Gao, Yan, Tan, Yulong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9598764/
https://www.ncbi.nlm.nih.gov/pubmed/36290061
http://dx.doi.org/10.3390/antibiotics11101403
_version_ 1784816432299638784
author Xu, Jiaman
Lin, Quan
Sheng, Maokun
Ding, Ting
Li, Bing
Gao, Yan
Tan, Yulong
author_facet Xu, Jiaman
Lin, Quan
Sheng, Maokun
Ding, Ting
Li, Bing
Gao, Yan
Tan, Yulong
author_sort Xu, Jiaman
collection PubMed
description Food contamination caused by food-spoilage bacteria and pathogenic bacteria seriously affects public health. Staphylococcus aureus is a typical foodborne pathogen which easily forms biofilm. Once biofilm is formed, it is difficult to remove. The use of nanotechnology for antibiofilm purposes is becoming more widespread because of its ability to increase the bioavailability and biosorption of many drugs. In this work, chitosan nanoparticles (CSNPs) were prepared by the ion–gel method with polyanionic sodium triphosphate (TPP). Cinnamaldehyde (CA) was loaded onto the CSNPs. The particle size, potential, morphology, encapsulation efficiency and in vitro release behavior of cinnamaldehyde–chitosan nanoparticles (CSNP-CAs) were studied, and the activity of CA against S. aureus biofilms was evaluated. The biofilm structure on the silicone surface was investigated by scanning electron microscopy (SEM). Confocal laser scanning microscopy (CLSM) was used to detect live/dead organisms within biofilms. The results showed that CSNP-CAs were dispersed in a circle with an average diameter of 298.1 nm and a zeta potential of +38.73 mV. The encapsulation efficiency of cinnamaldehyde (CA) reached 39.7%. In vitro release studies have shown that CA can be continuously released from the CSNPs. Compared with free drugs, CSNP-CAs have a higher efficacy in removing S. aureus biofilm, and the eradication rate of biofilm can reach 61%. The antibiofilm effects of CSNP-CAs are determined by their antibacterial properties. The minimum inhibitory concentration (MIC) of CA is 1.25 mg/mL; at this concentration the bacterial cell wall ruptures and the permeability of the cell membrane increases, which leads to leakage of the contents. At the same time, we verified that the MIC of CSNP-CAs is 2.5 mg/mL (drug concentration). The synergy between CA and CSNPs demonstrates the combinatorial application of a composite as an efficient novel therapeutic agent against antibiofilm. We can apply it in food preservation and other contexts, providing new ideas for food preservation.
format Online
Article
Text
id pubmed-9598764
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-95987642022-10-27 Antibiofilm Effect of Cinnamaldehyde-Chitosan Nanoparticles against the Biofilm of Staphylococcus aureus Xu, Jiaman Lin, Quan Sheng, Maokun Ding, Ting Li, Bing Gao, Yan Tan, Yulong Antibiotics (Basel) Article Food contamination caused by food-spoilage bacteria and pathogenic bacteria seriously affects public health. Staphylococcus aureus is a typical foodborne pathogen which easily forms biofilm. Once biofilm is formed, it is difficult to remove. The use of nanotechnology for antibiofilm purposes is becoming more widespread because of its ability to increase the bioavailability and biosorption of many drugs. In this work, chitosan nanoparticles (CSNPs) were prepared by the ion–gel method with polyanionic sodium triphosphate (TPP). Cinnamaldehyde (CA) was loaded onto the CSNPs. The particle size, potential, morphology, encapsulation efficiency and in vitro release behavior of cinnamaldehyde–chitosan nanoparticles (CSNP-CAs) were studied, and the activity of CA against S. aureus biofilms was evaluated. The biofilm structure on the silicone surface was investigated by scanning electron microscopy (SEM). Confocal laser scanning microscopy (CLSM) was used to detect live/dead organisms within biofilms. The results showed that CSNP-CAs were dispersed in a circle with an average diameter of 298.1 nm and a zeta potential of +38.73 mV. The encapsulation efficiency of cinnamaldehyde (CA) reached 39.7%. In vitro release studies have shown that CA can be continuously released from the CSNPs. Compared with free drugs, CSNP-CAs have a higher efficacy in removing S. aureus biofilm, and the eradication rate of biofilm can reach 61%. The antibiofilm effects of CSNP-CAs are determined by their antibacterial properties. The minimum inhibitory concentration (MIC) of CA is 1.25 mg/mL; at this concentration the bacterial cell wall ruptures and the permeability of the cell membrane increases, which leads to leakage of the contents. At the same time, we verified that the MIC of CSNP-CAs is 2.5 mg/mL (drug concentration). The synergy between CA and CSNPs demonstrates the combinatorial application of a composite as an efficient novel therapeutic agent against antibiofilm. We can apply it in food preservation and other contexts, providing new ideas for food preservation. MDPI 2022-10-13 /pmc/articles/PMC9598764/ /pubmed/36290061 http://dx.doi.org/10.3390/antibiotics11101403 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Xu, Jiaman
Lin, Quan
Sheng, Maokun
Ding, Ting
Li, Bing
Gao, Yan
Tan, Yulong
Antibiofilm Effect of Cinnamaldehyde-Chitosan Nanoparticles against the Biofilm of Staphylococcus aureus
title Antibiofilm Effect of Cinnamaldehyde-Chitosan Nanoparticles against the Biofilm of Staphylococcus aureus
title_full Antibiofilm Effect of Cinnamaldehyde-Chitosan Nanoparticles against the Biofilm of Staphylococcus aureus
title_fullStr Antibiofilm Effect of Cinnamaldehyde-Chitosan Nanoparticles against the Biofilm of Staphylococcus aureus
title_full_unstemmed Antibiofilm Effect of Cinnamaldehyde-Chitosan Nanoparticles against the Biofilm of Staphylococcus aureus
title_short Antibiofilm Effect of Cinnamaldehyde-Chitosan Nanoparticles against the Biofilm of Staphylococcus aureus
title_sort antibiofilm effect of cinnamaldehyde-chitosan nanoparticles against the biofilm of staphylococcus aureus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9598764/
https://www.ncbi.nlm.nih.gov/pubmed/36290061
http://dx.doi.org/10.3390/antibiotics11101403
work_keys_str_mv AT xujiaman antibiofilmeffectofcinnamaldehydechitosannanoparticlesagainstthebiofilmofstaphylococcusaureus
AT linquan antibiofilmeffectofcinnamaldehydechitosannanoparticlesagainstthebiofilmofstaphylococcusaureus
AT shengmaokun antibiofilmeffectofcinnamaldehydechitosannanoparticlesagainstthebiofilmofstaphylococcusaureus
AT dingting antibiofilmeffectofcinnamaldehydechitosannanoparticlesagainstthebiofilmofstaphylococcusaureus
AT libing antibiofilmeffectofcinnamaldehydechitosannanoparticlesagainstthebiofilmofstaphylococcusaureus
AT gaoyan antibiofilmeffectofcinnamaldehydechitosannanoparticlesagainstthebiofilmofstaphylococcusaureus
AT tanyulong antibiofilmeffectofcinnamaldehydechitosannanoparticlesagainstthebiofilmofstaphylococcusaureus