Questions about Residual Cell Viability in Cryopreserved Human Amniotic Membrane and Its Impact on Clinical Applications

We questioned the relevance of evaluating residual cell viability in human amniotic membrane (hAM) after its cryopreservation since cell survival is controversial and its ability to act as a matrix (including the presence of growth factors and cytokines) appears to be most important for tissue regen...

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Autores principales: Gaudet, Camille, Solecki, Lauriana, Mathéaud, Bastien, Odet, Stephane, Meyer, Christophe, Louvrier, Aurélien, Gindraux, Florelle
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Opinion
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9598775/
https://www.ncbi.nlm.nih.gov/pubmed/36289719
http://dx.doi.org/10.3390/biomedicines10102456
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author Gaudet, Camille
Solecki, Lauriana
Mathéaud, Bastien
Odet, Stephane
Meyer, Christophe
Louvrier, Aurélien
Gindraux, Florelle
author_facet Gaudet, Camille
Solecki, Lauriana
Mathéaud, Bastien
Odet, Stephane
Meyer, Christophe
Louvrier, Aurélien
Gindraux, Florelle
author_sort Gaudet, Camille
collection PubMed
description We questioned the relevance of evaluating residual cell viability in human amniotic membrane (hAM) after its cryopreservation since cell survival is controversial and its ability to act as a matrix (including the presence of growth factors and cytokines) appears to be most important for tissue regeneration purposes. We also discussed the usefulness of osteodifferentiating amniotic cells in whole hAM for bone repair applications. We have evidence that determining residual cell viability after cryopreservation and hAM osteodifferentiation is not justified.
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spelling pubmed-95987752022-10-27 Questions about Residual Cell Viability in Cryopreserved Human Amniotic Membrane and Its Impact on Clinical Applications Gaudet, Camille Solecki, Lauriana Mathéaud, Bastien Odet, Stephane Meyer, Christophe Louvrier, Aurélien Gindraux, Florelle Biomedicines Opinion We questioned the relevance of evaluating residual cell viability in human amniotic membrane (hAM) after its cryopreservation since cell survival is controversial and its ability to act as a matrix (including the presence of growth factors and cytokines) appears to be most important for tissue regeneration purposes. We also discussed the usefulness of osteodifferentiating amniotic cells in whole hAM for bone repair applications. We have evidence that determining residual cell viability after cryopreservation and hAM osteodifferentiation is not justified. MDPI 2022-10-01 /pmc/articles/PMC9598775/ /pubmed/36289719 http://dx.doi.org/10.3390/biomedicines10102456 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Opinion
Gaudet, Camille
Solecki, Lauriana
Mathéaud, Bastien
Odet, Stephane
Meyer, Christophe
Louvrier, Aurélien
Gindraux, Florelle
Questions about Residual Cell Viability in Cryopreserved Human Amniotic Membrane and Its Impact on Clinical Applications
title Questions about Residual Cell Viability in Cryopreserved Human Amniotic Membrane and Its Impact on Clinical Applications
title_full Questions about Residual Cell Viability in Cryopreserved Human Amniotic Membrane and Its Impact on Clinical Applications
title_fullStr Questions about Residual Cell Viability in Cryopreserved Human Amniotic Membrane and Its Impact on Clinical Applications
title_full_unstemmed Questions about Residual Cell Viability in Cryopreserved Human Amniotic Membrane and Its Impact on Clinical Applications
title_short Questions about Residual Cell Viability in Cryopreserved Human Amniotic Membrane and Its Impact on Clinical Applications
title_sort questions about residual cell viability in cryopreserved human amniotic membrane and its impact on clinical applications
topic Opinion
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9598775/
https://www.ncbi.nlm.nih.gov/pubmed/36289719
http://dx.doi.org/10.3390/biomedicines10102456
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