Cargando…

A Polyphenol-Rich Extract from Muscadine Grapes Prevents Hypertension-Induced Diastolic Dysfunction and Oxidative Stress

Muscadine grapes are abundant in dietary polyphenols, but their effect on hypertension-induced cardiac damage is limited. This study assessed whether a muscadine grape skin/seed extract supplement (MGES) prevents hypertension-induced cardiac damage and oxidative stress. Male Sprague Dawley rats were...

Descripción completa

Detalles Bibliográficos
Autores principales: Patil, Pooja D., Melo, Ana Clara, Westwood, Brian M., Tallant, E. Ann, Gallagher, Patricia E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9598776/
https://www.ncbi.nlm.nih.gov/pubmed/36290749
http://dx.doi.org/10.3390/antiox11102026
_version_ 1784816435223068672
author Patil, Pooja D.
Melo, Ana Clara
Westwood, Brian M.
Tallant, E. Ann
Gallagher, Patricia E.
author_facet Patil, Pooja D.
Melo, Ana Clara
Westwood, Brian M.
Tallant, E. Ann
Gallagher, Patricia E.
author_sort Patil, Pooja D.
collection PubMed
description Muscadine grapes are abundant in dietary polyphenols, but their effect on hypertension-induced cardiac damage is limited. This study assessed whether a muscadine grape skin/seed extract supplement (MGES) prevents hypertension-induced cardiac damage and oxidative stress. Male Sprague Dawley rats were treated for four weeks with drinking water, angiotensin II (Ang II) to induce hypertension, MGES, or both Ang II and MGES. Cardiac function assessed by echocardiography showed that Ang II increased systolic blood pressure while MGES alone or in combination with Ang II had no effect. Ang II increased E/e′, an indicator of left ventricular filling pressure and diastolic dysfunction, by 41% compared to Control and co-treatment with MGES prevented the Ang II-mediated increase, suggesting that the extract attenuated hypertension-induced diastolic function. Ang II infusion increased urinary 8-hydroxy-2′-deoxyguanosine and cardiac 4-hydroxynonenal and malondialdehyde, which were prevented by the extract. The antioxidant enzymes catalase and superoxide dismutase 1 activity and mRNA were increased significantly in animals treated with MGES alone or in combination with Ang II, suggesting that the extract upregulates oxidative stress defense mechanisms in cardiac tissue. Thus, MGES may serve as a medical food to protect the heart from hypertension-induced diastolic dysfunction caused in part by excessive reactive oxygen species production.
format Online
Article
Text
id pubmed-9598776
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-95987762022-10-27 A Polyphenol-Rich Extract from Muscadine Grapes Prevents Hypertension-Induced Diastolic Dysfunction and Oxidative Stress Patil, Pooja D. Melo, Ana Clara Westwood, Brian M. Tallant, E. Ann Gallagher, Patricia E. Antioxidants (Basel) Article Muscadine grapes are abundant in dietary polyphenols, but their effect on hypertension-induced cardiac damage is limited. This study assessed whether a muscadine grape skin/seed extract supplement (MGES) prevents hypertension-induced cardiac damage and oxidative stress. Male Sprague Dawley rats were treated for four weeks with drinking water, angiotensin II (Ang II) to induce hypertension, MGES, or both Ang II and MGES. Cardiac function assessed by echocardiography showed that Ang II increased systolic blood pressure while MGES alone or in combination with Ang II had no effect. Ang II increased E/e′, an indicator of left ventricular filling pressure and diastolic dysfunction, by 41% compared to Control and co-treatment with MGES prevented the Ang II-mediated increase, suggesting that the extract attenuated hypertension-induced diastolic function. Ang II infusion increased urinary 8-hydroxy-2′-deoxyguanosine and cardiac 4-hydroxynonenal and malondialdehyde, which were prevented by the extract. The antioxidant enzymes catalase and superoxide dismutase 1 activity and mRNA were increased significantly in animals treated with MGES alone or in combination with Ang II, suggesting that the extract upregulates oxidative stress defense mechanisms in cardiac tissue. Thus, MGES may serve as a medical food to protect the heart from hypertension-induced diastolic dysfunction caused in part by excessive reactive oxygen species production. MDPI 2022-10-14 /pmc/articles/PMC9598776/ /pubmed/36290749 http://dx.doi.org/10.3390/antiox11102026 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Patil, Pooja D.
Melo, Ana Clara
Westwood, Brian M.
Tallant, E. Ann
Gallagher, Patricia E.
A Polyphenol-Rich Extract from Muscadine Grapes Prevents Hypertension-Induced Diastolic Dysfunction and Oxidative Stress
title A Polyphenol-Rich Extract from Muscadine Grapes Prevents Hypertension-Induced Diastolic Dysfunction and Oxidative Stress
title_full A Polyphenol-Rich Extract from Muscadine Grapes Prevents Hypertension-Induced Diastolic Dysfunction and Oxidative Stress
title_fullStr A Polyphenol-Rich Extract from Muscadine Grapes Prevents Hypertension-Induced Diastolic Dysfunction and Oxidative Stress
title_full_unstemmed A Polyphenol-Rich Extract from Muscadine Grapes Prevents Hypertension-Induced Diastolic Dysfunction and Oxidative Stress
title_short A Polyphenol-Rich Extract from Muscadine Grapes Prevents Hypertension-Induced Diastolic Dysfunction and Oxidative Stress
title_sort polyphenol-rich extract from muscadine grapes prevents hypertension-induced diastolic dysfunction and oxidative stress
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9598776/
https://www.ncbi.nlm.nih.gov/pubmed/36290749
http://dx.doi.org/10.3390/antiox11102026
work_keys_str_mv AT patilpoojad apolyphenolrichextractfrommuscadinegrapespreventshypertensioninduceddiastolicdysfunctionandoxidativestress
AT meloanaclara apolyphenolrichextractfrommuscadinegrapespreventshypertensioninduceddiastolicdysfunctionandoxidativestress
AT westwoodbrianm apolyphenolrichextractfrommuscadinegrapespreventshypertensioninduceddiastolicdysfunctionandoxidativestress
AT tallanteann apolyphenolrichextractfrommuscadinegrapespreventshypertensioninduceddiastolicdysfunctionandoxidativestress
AT gallagherpatriciae apolyphenolrichextractfrommuscadinegrapespreventshypertensioninduceddiastolicdysfunctionandoxidativestress
AT patilpoojad polyphenolrichextractfrommuscadinegrapespreventshypertensioninduceddiastolicdysfunctionandoxidativestress
AT meloanaclara polyphenolrichextractfrommuscadinegrapespreventshypertensioninduceddiastolicdysfunctionandoxidativestress
AT westwoodbrianm polyphenolrichextractfrommuscadinegrapespreventshypertensioninduceddiastolicdysfunctionandoxidativestress
AT tallanteann polyphenolrichextractfrommuscadinegrapespreventshypertensioninduceddiastolicdysfunctionandoxidativestress
AT gallagherpatriciae polyphenolrichextractfrommuscadinegrapespreventshypertensioninduceddiastolicdysfunctionandoxidativestress