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Potentiation of Antibiotic Action and Efflux Pump Inhibitory Effect on Staphylococcus aureus Strains by Solasodine

A worrisome fact is the increase in microbial resistance, which has as its main cause the indiscriminate use of antibiotics. Scientific studies have investigated bioactive compounds such as steroidal sapogenins, in the perspective of new beneficial alternatives for the control of bacterial resistanc...

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Autores principales: da Silva, Ana Raquel Pereira, Costa, Maria do Socorro, Araújo, Nara Juliana Santos, de Freitas, Thiago Sampaio, de Almeida, Ray Silva, Barbosa Filho, José Maria, Tavares, Josean Fechine, de Souza, Erlânio Oliveira, de Farias, Pablo Antonio Maia, Pinheiro, Jacqueline Cosmo Andrade, Coutinho, Henrique Douglas Melo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9598803/
https://www.ncbi.nlm.nih.gov/pubmed/36289967
http://dx.doi.org/10.3390/antibiotics11101309
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author da Silva, Ana Raquel Pereira
Costa, Maria do Socorro
Araújo, Nara Juliana Santos
de Freitas, Thiago Sampaio
de Almeida, Ray Silva
Barbosa Filho, José Maria
Tavares, Josean Fechine
de Souza, Erlânio Oliveira
de Farias, Pablo Antonio Maia
Pinheiro, Jacqueline Cosmo Andrade
Coutinho, Henrique Douglas Melo
author_facet da Silva, Ana Raquel Pereira
Costa, Maria do Socorro
Araújo, Nara Juliana Santos
de Freitas, Thiago Sampaio
de Almeida, Ray Silva
Barbosa Filho, José Maria
Tavares, Josean Fechine
de Souza, Erlânio Oliveira
de Farias, Pablo Antonio Maia
Pinheiro, Jacqueline Cosmo Andrade
Coutinho, Henrique Douglas Melo
author_sort da Silva, Ana Raquel Pereira
collection PubMed
description A worrisome fact is the increase in microbial resistance, which has as its main cause the indiscriminate use of antibiotics. Scientific studies have investigated bioactive compounds such as steroidal sapogenins, in the perspective of new beneficial alternatives for the control of bacterial resistance. Therefore, the objective of this work was to verify the antibacterial activity as well as the modifying action of antibiotics associated with solasodine and its ability to inhibit the efflux pump mechanism in strains of Staphylococcus aureus. Tests were performed to verify the minimum inhibitory concentration (MIC). In addition, the action-modifying potential of antibiotics and the inhibitory capacity of the efflux pump NorA and MepA through synergistic effects on the antibiotic and ethidium bromide were evaluated. Solasodine showed significant results for the standard bacteria with an MIC of 512 μg/mL, and when associated with the antibiotics gentamicin and nofloxacin for the multidrug-resistant bacteria S. aureus 10, Escherichia coli 06, and Pseudomonas aeruginosa 24, it showed a 50% reduction in MIC. The association of solasodine with the antibiotic ciprofloxacin against S. aureus K2068 (MepA) showed synergism, with a reduction in the MIC of the antibiotic from 64 μg/mL to 40 μg/mL, and also a reduction in the MIC when the antibiotic was used in conjunction with the efflux pump inhibitors. Solasodine may be acting on the mechanism of action of the antibiotic, as it has shown a potentiating effect when associated with antibiotics, inducing a reduction in the MIC against Gram-positive and Gram-negative bacteria. Therefore, this study demonstrated significant results for the potentiating action of solasodine when associated with antibiotics of clinical importance.
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spelling pubmed-95988032022-10-27 Potentiation of Antibiotic Action and Efflux Pump Inhibitory Effect on Staphylococcus aureus Strains by Solasodine da Silva, Ana Raquel Pereira Costa, Maria do Socorro Araújo, Nara Juliana Santos de Freitas, Thiago Sampaio de Almeida, Ray Silva Barbosa Filho, José Maria Tavares, Josean Fechine de Souza, Erlânio Oliveira de Farias, Pablo Antonio Maia Pinheiro, Jacqueline Cosmo Andrade Coutinho, Henrique Douglas Melo Antibiotics (Basel) Article A worrisome fact is the increase in microbial resistance, which has as its main cause the indiscriminate use of antibiotics. Scientific studies have investigated bioactive compounds such as steroidal sapogenins, in the perspective of new beneficial alternatives for the control of bacterial resistance. Therefore, the objective of this work was to verify the antibacterial activity as well as the modifying action of antibiotics associated with solasodine and its ability to inhibit the efflux pump mechanism in strains of Staphylococcus aureus. Tests were performed to verify the minimum inhibitory concentration (MIC). In addition, the action-modifying potential of antibiotics and the inhibitory capacity of the efflux pump NorA and MepA through synergistic effects on the antibiotic and ethidium bromide were evaluated. Solasodine showed significant results for the standard bacteria with an MIC of 512 μg/mL, and when associated with the antibiotics gentamicin and nofloxacin for the multidrug-resistant bacteria S. aureus 10, Escherichia coli 06, and Pseudomonas aeruginosa 24, it showed a 50% reduction in MIC. The association of solasodine with the antibiotic ciprofloxacin against S. aureus K2068 (MepA) showed synergism, with a reduction in the MIC of the antibiotic from 64 μg/mL to 40 μg/mL, and also a reduction in the MIC when the antibiotic was used in conjunction with the efflux pump inhibitors. Solasodine may be acting on the mechanism of action of the antibiotic, as it has shown a potentiating effect when associated with antibiotics, inducing a reduction in the MIC against Gram-positive and Gram-negative bacteria. Therefore, this study demonstrated significant results for the potentiating action of solasodine when associated with antibiotics of clinical importance. MDPI 2022-09-27 /pmc/articles/PMC9598803/ /pubmed/36289967 http://dx.doi.org/10.3390/antibiotics11101309 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
da Silva, Ana Raquel Pereira
Costa, Maria do Socorro
Araújo, Nara Juliana Santos
de Freitas, Thiago Sampaio
de Almeida, Ray Silva
Barbosa Filho, José Maria
Tavares, Josean Fechine
de Souza, Erlânio Oliveira
de Farias, Pablo Antonio Maia
Pinheiro, Jacqueline Cosmo Andrade
Coutinho, Henrique Douglas Melo
Potentiation of Antibiotic Action and Efflux Pump Inhibitory Effect on Staphylococcus aureus Strains by Solasodine
title Potentiation of Antibiotic Action and Efflux Pump Inhibitory Effect on Staphylococcus aureus Strains by Solasodine
title_full Potentiation of Antibiotic Action and Efflux Pump Inhibitory Effect on Staphylococcus aureus Strains by Solasodine
title_fullStr Potentiation of Antibiotic Action and Efflux Pump Inhibitory Effect on Staphylococcus aureus Strains by Solasodine
title_full_unstemmed Potentiation of Antibiotic Action and Efflux Pump Inhibitory Effect on Staphylococcus aureus Strains by Solasodine
title_short Potentiation of Antibiotic Action and Efflux Pump Inhibitory Effect on Staphylococcus aureus Strains by Solasodine
title_sort potentiation of antibiotic action and efflux pump inhibitory effect on staphylococcus aureus strains by solasodine
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9598803/
https://www.ncbi.nlm.nih.gov/pubmed/36289967
http://dx.doi.org/10.3390/antibiotics11101309
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