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Antimicrobial Activity of Blow Spun PLA/Gelatin Nanofibers Containing Green Synthesized Silver Nanoparticles against Wound Infection-Causing Bacteria

One of the main challenges in wound healing is the wound infection due to various causes, of which moisture is the most important reason. Owing to this fact, wound dressings that can collect wound moisture in addition to showing antibacterial properties have provided an important basis for wound hea...

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Autores principales: Alinezhad Sardareh, Elham, Shahzeidi, Moloud, Salmanifard Ardestani, Mohammad Taha, Mousavi-Khattat, Mohammad, Zarepour, Atefeh, Zarrabi, Ali
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9599005/
https://www.ncbi.nlm.nih.gov/pubmed/36290486
http://dx.doi.org/10.3390/bioengineering9100518
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author Alinezhad Sardareh, Elham
Shahzeidi, Moloud
Salmanifard Ardestani, Mohammad Taha
Mousavi-Khattat, Mohammad
Zarepour, Atefeh
Zarrabi, Ali
author_facet Alinezhad Sardareh, Elham
Shahzeidi, Moloud
Salmanifard Ardestani, Mohammad Taha
Mousavi-Khattat, Mohammad
Zarepour, Atefeh
Zarrabi, Ali
author_sort Alinezhad Sardareh, Elham
collection PubMed
description One of the main challenges in wound healing is the wound infection due to various causes, of which moisture is the most important reason. Owing to this fact, wound dressings that can collect wound moisture in addition to showing antibacterial properties have provided an important basis for wound healing research. In this study, gelatin and poly lactic acid (PLA) polymers were used in a wound dressing textile to provide gelation and structure strength properties, respectively. Meanwhile, silver nanoparticles (SNPs) synthesized through the green method were integrated into these fibers to provide the formed textile with antibacterial properties. Nanoparticles were made using donkey dung extract, and nanofibers were produced by the solution blow spinning method which has high production efficiency and low energy consumption among spinning methods. The produced nanoparticles were characterized and evaluated by UV-Vis, DLS, XRD, and FTIR methods, and the production of silver nanoparticles that were coated with metabolites in the extract was proven. In addition, the morphology and diameter of the resulted fibers and presence of nanoparticles were confirmed by the SEM method. The size and size distribution of the synthesized fibers were determined through analyzing SEM results. Gelatin nanofibers demonstrated a mean size of 743 nm before and 773 nm after nanoparticle coating. PLA nanofibers demonstrated a mean size of 57 nm before and 182 nm after nanoparticle coating. Finally, 335 nm was the mean diameter size of gelatin/PLA/SNPs nanofibers. Also, the textiles synthesized by PLA and gelatin which contained silver nanoparticles showed higher antibacterial activity against both gram-positive and gram-negative species compared to PLA and gelatin tissues without nanoparticles. Cytotoxicity test on L929 cells showed that silver nanoparticles incorporated textiles of PLA and gelatin show a very low level and non-significant toxicity compared to the free particles.
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spelling pubmed-95990052022-10-27 Antimicrobial Activity of Blow Spun PLA/Gelatin Nanofibers Containing Green Synthesized Silver Nanoparticles against Wound Infection-Causing Bacteria Alinezhad Sardareh, Elham Shahzeidi, Moloud Salmanifard Ardestani, Mohammad Taha Mousavi-Khattat, Mohammad Zarepour, Atefeh Zarrabi, Ali Bioengineering (Basel) Article One of the main challenges in wound healing is the wound infection due to various causes, of which moisture is the most important reason. Owing to this fact, wound dressings that can collect wound moisture in addition to showing antibacterial properties have provided an important basis for wound healing research. In this study, gelatin and poly lactic acid (PLA) polymers were used in a wound dressing textile to provide gelation and structure strength properties, respectively. Meanwhile, silver nanoparticles (SNPs) synthesized through the green method were integrated into these fibers to provide the formed textile with antibacterial properties. Nanoparticles were made using donkey dung extract, and nanofibers were produced by the solution blow spinning method which has high production efficiency and low energy consumption among spinning methods. The produced nanoparticles were characterized and evaluated by UV-Vis, DLS, XRD, and FTIR methods, and the production of silver nanoparticles that were coated with metabolites in the extract was proven. In addition, the morphology and diameter of the resulted fibers and presence of nanoparticles were confirmed by the SEM method. The size and size distribution of the synthesized fibers were determined through analyzing SEM results. Gelatin nanofibers demonstrated a mean size of 743 nm before and 773 nm after nanoparticle coating. PLA nanofibers demonstrated a mean size of 57 nm before and 182 nm after nanoparticle coating. Finally, 335 nm was the mean diameter size of gelatin/PLA/SNPs nanofibers. Also, the textiles synthesized by PLA and gelatin which contained silver nanoparticles showed higher antibacterial activity against both gram-positive and gram-negative species compared to PLA and gelatin tissues without nanoparticles. Cytotoxicity test on L929 cells showed that silver nanoparticles incorporated textiles of PLA and gelatin show a very low level and non-significant toxicity compared to the free particles. MDPI 2022-10-01 /pmc/articles/PMC9599005/ /pubmed/36290486 http://dx.doi.org/10.3390/bioengineering9100518 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Alinezhad Sardareh, Elham
Shahzeidi, Moloud
Salmanifard Ardestani, Mohammad Taha
Mousavi-Khattat, Mohammad
Zarepour, Atefeh
Zarrabi, Ali
Antimicrobial Activity of Blow Spun PLA/Gelatin Nanofibers Containing Green Synthesized Silver Nanoparticles against Wound Infection-Causing Bacteria
title Antimicrobial Activity of Blow Spun PLA/Gelatin Nanofibers Containing Green Synthesized Silver Nanoparticles against Wound Infection-Causing Bacteria
title_full Antimicrobial Activity of Blow Spun PLA/Gelatin Nanofibers Containing Green Synthesized Silver Nanoparticles against Wound Infection-Causing Bacteria
title_fullStr Antimicrobial Activity of Blow Spun PLA/Gelatin Nanofibers Containing Green Synthesized Silver Nanoparticles against Wound Infection-Causing Bacteria
title_full_unstemmed Antimicrobial Activity of Blow Spun PLA/Gelatin Nanofibers Containing Green Synthesized Silver Nanoparticles against Wound Infection-Causing Bacteria
title_short Antimicrobial Activity of Blow Spun PLA/Gelatin Nanofibers Containing Green Synthesized Silver Nanoparticles against Wound Infection-Causing Bacteria
title_sort antimicrobial activity of blow spun pla/gelatin nanofibers containing green synthesized silver nanoparticles against wound infection-causing bacteria
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9599005/
https://www.ncbi.nlm.nih.gov/pubmed/36290486
http://dx.doi.org/10.3390/bioengineering9100518
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