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Quercetin improves lacrimal gland function through its anti-oxidant actions: Evidence from animal studies, and a pilot study in healthy human volunteers

Anti-oxidant properties of polyphenols have been gaining medical attention as a preventive factor against aging and/or lifestyle diseases. In this study, we examined the anti-oxidant activity of quercetin improved tear function through its effects on the lacrimal gland in mice and humans. Six week-o...

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Autores principales: Inaba, Takaaki, Ohnishi-Kameyama, Mayumi, Liu, Ying, Tanaka, Yasuhisa, Kobori, Masuko, Tamaki, Shusaku, Ito, Tomotaka, Higa, Kazunari, Shimazaki, Jun, Tsubota, Kazuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9599396/
https://www.ncbi.nlm.nih.gov/pubmed/36313100
http://dx.doi.org/10.3389/fnut.2022.974530
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author Inaba, Takaaki
Ohnishi-Kameyama, Mayumi
Liu, Ying
Tanaka, Yasuhisa
Kobori, Masuko
Tamaki, Shusaku
Ito, Tomotaka
Higa, Kazunari
Shimazaki, Jun
Tsubota, Kazuo
author_facet Inaba, Takaaki
Ohnishi-Kameyama, Mayumi
Liu, Ying
Tanaka, Yasuhisa
Kobori, Masuko
Tamaki, Shusaku
Ito, Tomotaka
Higa, Kazunari
Shimazaki, Jun
Tsubota, Kazuo
author_sort Inaba, Takaaki
collection PubMed
description Anti-oxidant properties of polyphenols have been gaining medical attention as a preventive factor against aging and/or lifestyle diseases. In this study, we examined the anti-oxidant activity of quercetin improved tear function through its effects on the lacrimal gland in mice and humans. Six week-old diabetic mice, a model for decreased tear production, were fed for 12 weeks ad libitum with an experimental diet containing 0.5% quercetin. As a result, the tear volume was significantly improved compared to the control, despite no changes in body weight, food intake, lacrimal gland morphology or biochemical serum parameters. Moreover, significantly higher SOD-1 and SOD-2 protein levels were detected in the lacrimal glands of quercetin-treated mice by western blot. In addition, quercetin treatment of mouse corneal cell lines exposed to oxidative stress resulted in dose-dependent inhibition of ROS production and enhanced cell survival. Finally, we examined quercetin pharmacokinetics, specifically its presence in serum and tears subsequent to onion consumption in healthy volunteers, and found that the distribution of quercetin and its metabolite shifted from serum to tear following onion intake. An improvement in tear film stability also resulted following the intake by these healthy volunteers of a new, quercetin-rich onion cultivar (”Quergold”) in powder form. These results suggested that quercetin improved tear function through its effects on the lacrimal gland in mice and humans.
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spelling pubmed-95993962022-10-27 Quercetin improves lacrimal gland function through its anti-oxidant actions: Evidence from animal studies, and a pilot study in healthy human volunteers Inaba, Takaaki Ohnishi-Kameyama, Mayumi Liu, Ying Tanaka, Yasuhisa Kobori, Masuko Tamaki, Shusaku Ito, Tomotaka Higa, Kazunari Shimazaki, Jun Tsubota, Kazuo Front Nutr Nutrition Anti-oxidant properties of polyphenols have been gaining medical attention as a preventive factor against aging and/or lifestyle diseases. In this study, we examined the anti-oxidant activity of quercetin improved tear function through its effects on the lacrimal gland in mice and humans. Six week-old diabetic mice, a model for decreased tear production, were fed for 12 weeks ad libitum with an experimental diet containing 0.5% quercetin. As a result, the tear volume was significantly improved compared to the control, despite no changes in body weight, food intake, lacrimal gland morphology or biochemical serum parameters. Moreover, significantly higher SOD-1 and SOD-2 protein levels were detected in the lacrimal glands of quercetin-treated mice by western blot. In addition, quercetin treatment of mouse corneal cell lines exposed to oxidative stress resulted in dose-dependent inhibition of ROS production and enhanced cell survival. Finally, we examined quercetin pharmacokinetics, specifically its presence in serum and tears subsequent to onion consumption in healthy volunteers, and found that the distribution of quercetin and its metabolite shifted from serum to tear following onion intake. An improvement in tear film stability also resulted following the intake by these healthy volunteers of a new, quercetin-rich onion cultivar (”Quergold”) in powder form. These results suggested that quercetin improved tear function through its effects on the lacrimal gland in mice and humans. Frontiers Media S.A. 2022-10-06 /pmc/articles/PMC9599396/ /pubmed/36313100 http://dx.doi.org/10.3389/fnut.2022.974530 Text en Copyright © 2022 Inaba, Ohnishi-Kameyama, Liu, Tanaka, Kobori, Tamaki, Ito, Higa, Shimazaki and Tsubota. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Nutrition
Inaba, Takaaki
Ohnishi-Kameyama, Mayumi
Liu, Ying
Tanaka, Yasuhisa
Kobori, Masuko
Tamaki, Shusaku
Ito, Tomotaka
Higa, Kazunari
Shimazaki, Jun
Tsubota, Kazuo
Quercetin improves lacrimal gland function through its anti-oxidant actions: Evidence from animal studies, and a pilot study in healthy human volunteers
title Quercetin improves lacrimal gland function through its anti-oxidant actions: Evidence from animal studies, and a pilot study in healthy human volunteers
title_full Quercetin improves lacrimal gland function through its anti-oxidant actions: Evidence from animal studies, and a pilot study in healthy human volunteers
title_fullStr Quercetin improves lacrimal gland function through its anti-oxidant actions: Evidence from animal studies, and a pilot study in healthy human volunteers
title_full_unstemmed Quercetin improves lacrimal gland function through its anti-oxidant actions: Evidence from animal studies, and a pilot study in healthy human volunteers
title_short Quercetin improves lacrimal gland function through its anti-oxidant actions: Evidence from animal studies, and a pilot study in healthy human volunteers
title_sort quercetin improves lacrimal gland function through its anti-oxidant actions: evidence from animal studies, and a pilot study in healthy human volunteers
topic Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9599396/
https://www.ncbi.nlm.nih.gov/pubmed/36313100
http://dx.doi.org/10.3389/fnut.2022.974530
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