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Plasma and Peritoneal Fluid ZEB Levels in Patients with Endometriosis and Infertility
Zinc finger E-box-binding homeobox 1 (ZEB1) and zinc finger E-box-binding homeobox 2 (ZEB2) are transcription factors that regulate epithelial–mesenchymal transformation (EMT). The aim of this study was to compare levels of ZEB1 and ZEB2 in the peritoneal fluid and plasma between patients with and w...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9599446/ https://www.ncbi.nlm.nih.gov/pubmed/36289723 http://dx.doi.org/10.3390/biomedicines10102460 |
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author | Bartnik, Paweł Kacperczyk-Bartnik, Joanna Goławski, Ksawery Sierdziński, Janusz Mańka, Grzegorz Kiecka, Mariusz Lipa, Michał Warzecha, Damian Spaczyński, Robert Piekarski, Piotr Banaszewska, Beata Jakimiuk, Artur J. Issat, Tadeusz Rokita, Wojciech Młodawski, Jakub Szubert, Maria Sieroszewski, Piotr Raba, Grzegorz Szczupak, Kamil Kluz, Tomasz Kluza, Marek Czajkowski, Krzysztof Wielgoś, Mirosław Koc-Żórawska, Ewa Żórawski, Marcin Laudański, Piotr |
author_facet | Bartnik, Paweł Kacperczyk-Bartnik, Joanna Goławski, Ksawery Sierdziński, Janusz Mańka, Grzegorz Kiecka, Mariusz Lipa, Michał Warzecha, Damian Spaczyński, Robert Piekarski, Piotr Banaszewska, Beata Jakimiuk, Artur J. Issat, Tadeusz Rokita, Wojciech Młodawski, Jakub Szubert, Maria Sieroszewski, Piotr Raba, Grzegorz Szczupak, Kamil Kluz, Tomasz Kluza, Marek Czajkowski, Krzysztof Wielgoś, Mirosław Koc-Żórawska, Ewa Żórawski, Marcin Laudański, Piotr |
author_sort | Bartnik, Paweł |
collection | PubMed |
description | Zinc finger E-box-binding homeobox 1 (ZEB1) and zinc finger E-box-binding homeobox 2 (ZEB2) are transcription factors that regulate epithelial–mesenchymal transformation (EMT). The aim of this study was to compare levels of ZEB1 and ZEB2 in the peritoneal fluid and plasma between patients with and without endometriosis in order to assess their utility in the diagnostic process. Plasma and peritoneal fluid samples were collected from 50 patients with and 48 without endometriosis during planned surgical procedures in eight clinical centers. Quantitative ZEB1 and ZEB2 levels analyses were performed using a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). No significant differences were observed in ZEB1 levels in any of the subanalyses nor any differences regarding ZEB2 levels between patients with and without endometriosis. Plasma ZEB2 levels were significantly higher among patients with infertility compared to fertile women (16.07 ± 12.70 ng/L vs. 12.07 ± 11.92 ng/L; p < 0.04). Both ZEB1 and ZEB2 do not seem to have a significant value in the initial diagnosis of endometriosis as a single marker. The differences in ZEB2 plasma levels between patients with and without infertility indicate the possibility of EMT dysregulation in the pathogenesis of adverse fertility outcomes. |
format | Online Article Text |
id | pubmed-9599446 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-95994462022-10-27 Plasma and Peritoneal Fluid ZEB Levels in Patients with Endometriosis and Infertility Bartnik, Paweł Kacperczyk-Bartnik, Joanna Goławski, Ksawery Sierdziński, Janusz Mańka, Grzegorz Kiecka, Mariusz Lipa, Michał Warzecha, Damian Spaczyński, Robert Piekarski, Piotr Banaszewska, Beata Jakimiuk, Artur J. Issat, Tadeusz Rokita, Wojciech Młodawski, Jakub Szubert, Maria Sieroszewski, Piotr Raba, Grzegorz Szczupak, Kamil Kluz, Tomasz Kluza, Marek Czajkowski, Krzysztof Wielgoś, Mirosław Koc-Żórawska, Ewa Żórawski, Marcin Laudański, Piotr Biomedicines Article Zinc finger E-box-binding homeobox 1 (ZEB1) and zinc finger E-box-binding homeobox 2 (ZEB2) are transcription factors that regulate epithelial–mesenchymal transformation (EMT). The aim of this study was to compare levels of ZEB1 and ZEB2 in the peritoneal fluid and plasma between patients with and without endometriosis in order to assess their utility in the diagnostic process. Plasma and peritoneal fluid samples were collected from 50 patients with and 48 without endometriosis during planned surgical procedures in eight clinical centers. Quantitative ZEB1 and ZEB2 levels analyses were performed using a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). No significant differences were observed in ZEB1 levels in any of the subanalyses nor any differences regarding ZEB2 levels between patients with and without endometriosis. Plasma ZEB2 levels were significantly higher among patients with infertility compared to fertile women (16.07 ± 12.70 ng/L vs. 12.07 ± 11.92 ng/L; p < 0.04). Both ZEB1 and ZEB2 do not seem to have a significant value in the initial diagnosis of endometriosis as a single marker. The differences in ZEB2 plasma levels between patients with and without infertility indicate the possibility of EMT dysregulation in the pathogenesis of adverse fertility outcomes. MDPI 2022-10-01 /pmc/articles/PMC9599446/ /pubmed/36289723 http://dx.doi.org/10.3390/biomedicines10102460 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Bartnik, Paweł Kacperczyk-Bartnik, Joanna Goławski, Ksawery Sierdziński, Janusz Mańka, Grzegorz Kiecka, Mariusz Lipa, Michał Warzecha, Damian Spaczyński, Robert Piekarski, Piotr Banaszewska, Beata Jakimiuk, Artur J. Issat, Tadeusz Rokita, Wojciech Młodawski, Jakub Szubert, Maria Sieroszewski, Piotr Raba, Grzegorz Szczupak, Kamil Kluz, Tomasz Kluza, Marek Czajkowski, Krzysztof Wielgoś, Mirosław Koc-Żórawska, Ewa Żórawski, Marcin Laudański, Piotr Plasma and Peritoneal Fluid ZEB Levels in Patients with Endometriosis and Infertility |
title | Plasma and Peritoneal Fluid ZEB Levels in Patients with Endometriosis and Infertility |
title_full | Plasma and Peritoneal Fluid ZEB Levels in Patients with Endometriosis and Infertility |
title_fullStr | Plasma and Peritoneal Fluid ZEB Levels in Patients with Endometriosis and Infertility |
title_full_unstemmed | Plasma and Peritoneal Fluid ZEB Levels in Patients with Endometriosis and Infertility |
title_short | Plasma and Peritoneal Fluid ZEB Levels in Patients with Endometriosis and Infertility |
title_sort | plasma and peritoneal fluid zeb levels in patients with endometriosis and infertility |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9599446/ https://www.ncbi.nlm.nih.gov/pubmed/36289723 http://dx.doi.org/10.3390/biomedicines10102460 |
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