Cargando…

Dual-Color Fluorescent Hydrogel Microspheres Combined with Smartphones for Visual Detection of Lactate

Since it is difficult for human eyes to distinguish between two identical colors with only <15% variation in brightness, mono-color fluorescent hydrogel microspheres have some limitations in the detection of lactate. Herein, we prepared novel dual-color fluorescent hydrogel microspheres, which ca...

Descripción completa

Detalles Bibliográficos
Autores principales: Yang, Sisi, Tang, Ziwen, Tian, Yilong, Ji, Xinghu, Wang, Fubing, Xie, Conghua, He, Zhike
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9599631/
https://www.ncbi.nlm.nih.gov/pubmed/36290939
http://dx.doi.org/10.3390/bios12100802
_version_ 1784816641268252672
author Yang, Sisi
Tang, Ziwen
Tian, Yilong
Ji, Xinghu
Wang, Fubing
Xie, Conghua
He, Zhike
author_facet Yang, Sisi
Tang, Ziwen
Tian, Yilong
Ji, Xinghu
Wang, Fubing
Xie, Conghua
He, Zhike
author_sort Yang, Sisi
collection PubMed
description Since it is difficult for human eyes to distinguish between two identical colors with only <15% variation in brightness, mono-color fluorescent hydrogel microspheres have some limitations in the detection of lactate. Herein, we prepared novel dual-color fluorescent hydrogel microspheres, which can achieve hue transformation. Microspheres were prepared by introducing a fluorescent nanoparticle as the reference signal while CdTe QDs were used as the response signal. We used smartphones with image processing software to collect and analyze data. In this way, the signal of lactate was converted to RGB (red, green, and blue) values, which can be quantitatively read. Within 10 to 1500 μM, the R/G values of the microspheres had a linear relationship with the logarithm of the lactate concentration. Moreover, color cards for lactate detection were prepared, from which the color change and concentration of lactate could be easily read by the naked eye. It is worth mentioning that this method was successfully applied to screen patients with hyperlactatemia.
format Online
Article
Text
id pubmed-9599631
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-95996312022-10-27 Dual-Color Fluorescent Hydrogel Microspheres Combined with Smartphones for Visual Detection of Lactate Yang, Sisi Tang, Ziwen Tian, Yilong Ji, Xinghu Wang, Fubing Xie, Conghua He, Zhike Biosensors (Basel) Article Since it is difficult for human eyes to distinguish between two identical colors with only <15% variation in brightness, mono-color fluorescent hydrogel microspheres have some limitations in the detection of lactate. Herein, we prepared novel dual-color fluorescent hydrogel microspheres, which can achieve hue transformation. Microspheres were prepared by introducing a fluorescent nanoparticle as the reference signal while CdTe QDs were used as the response signal. We used smartphones with image processing software to collect and analyze data. In this way, the signal of lactate was converted to RGB (red, green, and blue) values, which can be quantitatively read. Within 10 to 1500 μM, the R/G values of the microspheres had a linear relationship with the logarithm of the lactate concentration. Moreover, color cards for lactate detection were prepared, from which the color change and concentration of lactate could be easily read by the naked eye. It is worth mentioning that this method was successfully applied to screen patients with hyperlactatemia. MDPI 2022-09-28 /pmc/articles/PMC9599631/ /pubmed/36290939 http://dx.doi.org/10.3390/bios12100802 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yang, Sisi
Tang, Ziwen
Tian, Yilong
Ji, Xinghu
Wang, Fubing
Xie, Conghua
He, Zhike
Dual-Color Fluorescent Hydrogel Microspheres Combined with Smartphones for Visual Detection of Lactate
title Dual-Color Fluorescent Hydrogel Microspheres Combined with Smartphones for Visual Detection of Lactate
title_full Dual-Color Fluorescent Hydrogel Microspheres Combined with Smartphones for Visual Detection of Lactate
title_fullStr Dual-Color Fluorescent Hydrogel Microspheres Combined with Smartphones for Visual Detection of Lactate
title_full_unstemmed Dual-Color Fluorescent Hydrogel Microspheres Combined with Smartphones for Visual Detection of Lactate
title_short Dual-Color Fluorescent Hydrogel Microspheres Combined with Smartphones for Visual Detection of Lactate
title_sort dual-color fluorescent hydrogel microspheres combined with smartphones for visual detection of lactate
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9599631/
https://www.ncbi.nlm.nih.gov/pubmed/36290939
http://dx.doi.org/10.3390/bios12100802
work_keys_str_mv AT yangsisi dualcolorfluorescenthydrogelmicrospherescombinedwithsmartphonesforvisualdetectionoflactate
AT tangziwen dualcolorfluorescenthydrogelmicrospherescombinedwithsmartphonesforvisualdetectionoflactate
AT tianyilong dualcolorfluorescenthydrogelmicrospherescombinedwithsmartphonesforvisualdetectionoflactate
AT jixinghu dualcolorfluorescenthydrogelmicrospherescombinedwithsmartphonesforvisualdetectionoflactate
AT wangfubing dualcolorfluorescenthydrogelmicrospherescombinedwithsmartphonesforvisualdetectionoflactate
AT xieconghua dualcolorfluorescenthydrogelmicrospherescombinedwithsmartphonesforvisualdetectionoflactate
AT hezhike dualcolorfluorescenthydrogelmicrospherescombinedwithsmartphonesforvisualdetectionoflactate