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Dual-Color Fluorescent Hydrogel Microspheres Combined with Smartphones for Visual Detection of Lactate
Since it is difficult for human eyes to distinguish between two identical colors with only <15% variation in brightness, mono-color fluorescent hydrogel microspheres have some limitations in the detection of lactate. Herein, we prepared novel dual-color fluorescent hydrogel microspheres, which ca...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9599631/ https://www.ncbi.nlm.nih.gov/pubmed/36290939 http://dx.doi.org/10.3390/bios12100802 |
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author | Yang, Sisi Tang, Ziwen Tian, Yilong Ji, Xinghu Wang, Fubing Xie, Conghua He, Zhike |
author_facet | Yang, Sisi Tang, Ziwen Tian, Yilong Ji, Xinghu Wang, Fubing Xie, Conghua He, Zhike |
author_sort | Yang, Sisi |
collection | PubMed |
description | Since it is difficult for human eyes to distinguish between two identical colors with only <15% variation in brightness, mono-color fluorescent hydrogel microspheres have some limitations in the detection of lactate. Herein, we prepared novel dual-color fluorescent hydrogel microspheres, which can achieve hue transformation. Microspheres were prepared by introducing a fluorescent nanoparticle as the reference signal while CdTe QDs were used as the response signal. We used smartphones with image processing software to collect and analyze data. In this way, the signal of lactate was converted to RGB (red, green, and blue) values, which can be quantitatively read. Within 10 to 1500 μM, the R/G values of the microspheres had a linear relationship with the logarithm of the lactate concentration. Moreover, color cards for lactate detection were prepared, from which the color change and concentration of lactate could be easily read by the naked eye. It is worth mentioning that this method was successfully applied to screen patients with hyperlactatemia. |
format | Online Article Text |
id | pubmed-9599631 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-95996312022-10-27 Dual-Color Fluorescent Hydrogel Microspheres Combined with Smartphones for Visual Detection of Lactate Yang, Sisi Tang, Ziwen Tian, Yilong Ji, Xinghu Wang, Fubing Xie, Conghua He, Zhike Biosensors (Basel) Article Since it is difficult for human eyes to distinguish between two identical colors with only <15% variation in brightness, mono-color fluorescent hydrogel microspheres have some limitations in the detection of lactate. Herein, we prepared novel dual-color fluorescent hydrogel microspheres, which can achieve hue transformation. Microspheres were prepared by introducing a fluorescent nanoparticle as the reference signal while CdTe QDs were used as the response signal. We used smartphones with image processing software to collect and analyze data. In this way, the signal of lactate was converted to RGB (red, green, and blue) values, which can be quantitatively read. Within 10 to 1500 μM, the R/G values of the microspheres had a linear relationship with the logarithm of the lactate concentration. Moreover, color cards for lactate detection were prepared, from which the color change and concentration of lactate could be easily read by the naked eye. It is worth mentioning that this method was successfully applied to screen patients with hyperlactatemia. MDPI 2022-09-28 /pmc/articles/PMC9599631/ /pubmed/36290939 http://dx.doi.org/10.3390/bios12100802 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yang, Sisi Tang, Ziwen Tian, Yilong Ji, Xinghu Wang, Fubing Xie, Conghua He, Zhike Dual-Color Fluorescent Hydrogel Microspheres Combined with Smartphones for Visual Detection of Lactate |
title | Dual-Color Fluorescent Hydrogel Microspheres Combined with Smartphones for Visual Detection of Lactate |
title_full | Dual-Color Fluorescent Hydrogel Microspheres Combined with Smartphones for Visual Detection of Lactate |
title_fullStr | Dual-Color Fluorescent Hydrogel Microspheres Combined with Smartphones for Visual Detection of Lactate |
title_full_unstemmed | Dual-Color Fluorescent Hydrogel Microspheres Combined with Smartphones for Visual Detection of Lactate |
title_short | Dual-Color Fluorescent Hydrogel Microspheres Combined with Smartphones for Visual Detection of Lactate |
title_sort | dual-color fluorescent hydrogel microspheres combined with smartphones for visual detection of lactate |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9599631/ https://www.ncbi.nlm.nih.gov/pubmed/36290939 http://dx.doi.org/10.3390/bios12100802 |
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