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The Role of Probiotic Bacillus Spores and Amino Acids with Immunoglobulins on a Rat Enteropathy Model

Non-steroidal anti-inflammatory drugs (NSAIDs) are some of the most widely used drugs due to their anti-inflammatory, analgesic and antipyretic pharmacological effects. Gastrointestinal side effects are some of the most severe and frequent side effects of NSAIDs. These depend on the balance of the g...

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Autores principales: Neag, Maria-Adriana, Melincovici, Carmen-Stanca, Catinean, Adrian, Muntean, Dana-Maria, Pop, Raluca-Maria, Bocsan, Ioana-Corina, Mitre, Andrei-Otto, Cardos, Mihai-Bogdan, Inceu, Andreea-Ioana, Buzoianu, Anca-Dana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9599762/
https://www.ncbi.nlm.nih.gov/pubmed/36289770
http://dx.doi.org/10.3390/biomedicines10102508
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author Neag, Maria-Adriana
Melincovici, Carmen-Stanca
Catinean, Adrian
Muntean, Dana-Maria
Pop, Raluca-Maria
Bocsan, Ioana-Corina
Mitre, Andrei-Otto
Cardos, Mihai-Bogdan
Inceu, Andreea-Ioana
Buzoianu, Anca-Dana
author_facet Neag, Maria-Adriana
Melincovici, Carmen-Stanca
Catinean, Adrian
Muntean, Dana-Maria
Pop, Raluca-Maria
Bocsan, Ioana-Corina
Mitre, Andrei-Otto
Cardos, Mihai-Bogdan
Inceu, Andreea-Ioana
Buzoianu, Anca-Dana
author_sort Neag, Maria-Adriana
collection PubMed
description Non-steroidal anti-inflammatory drugs (NSAIDs) are some of the most widely used drugs due to their anti-inflammatory, analgesic and antipyretic pharmacological effects. Gastrointestinal side effects are some of the most severe and frequent side effects of NSAIDs. These depend on the balance of the gut microbiome, the abundance of Gram-negative bacteria, and the amount of lipopolysaccharide released. Therefore, restoring or improving gut bacteria balance with probiotic supplements could prove to be an adjuvant therapy against mild NSAID-induced enteropathy. Twenty-five Wistar albino male rats were divided into five groups. The negative control group was administered carboxymethylcellulose and the positive control group diclofenac (DIC), 8 mg/kg for 7 days, which represented the enteropathy model. Treatment groups consisted of a combination of pro-biotic spores (MSB), amino acids and immunoglobulins supplement (MM), which were also administered for 7 days. We analyzed hepatic injury markers (AST, ALT) and creatinine, and inflammatory markers, IL-6, TNF-α, PGE2, iNOS, as well as total antioxidant capacity. The results obtained in the present study suggest that the modulation of the intestinal microbiota by administration of probiotics (Bacillus spores), alone or in combination with immunoglobulins and amino acids, represents an attractive therapy for the prevention of NSAID-induced enteropathy.
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spelling pubmed-95997622022-10-27 The Role of Probiotic Bacillus Spores and Amino Acids with Immunoglobulins on a Rat Enteropathy Model Neag, Maria-Adriana Melincovici, Carmen-Stanca Catinean, Adrian Muntean, Dana-Maria Pop, Raluca-Maria Bocsan, Ioana-Corina Mitre, Andrei-Otto Cardos, Mihai-Bogdan Inceu, Andreea-Ioana Buzoianu, Anca-Dana Biomedicines Article Non-steroidal anti-inflammatory drugs (NSAIDs) are some of the most widely used drugs due to their anti-inflammatory, analgesic and antipyretic pharmacological effects. Gastrointestinal side effects are some of the most severe and frequent side effects of NSAIDs. These depend on the balance of the gut microbiome, the abundance of Gram-negative bacteria, and the amount of lipopolysaccharide released. Therefore, restoring or improving gut bacteria balance with probiotic supplements could prove to be an adjuvant therapy against mild NSAID-induced enteropathy. Twenty-five Wistar albino male rats were divided into five groups. The negative control group was administered carboxymethylcellulose and the positive control group diclofenac (DIC), 8 mg/kg for 7 days, which represented the enteropathy model. Treatment groups consisted of a combination of pro-biotic spores (MSB), amino acids and immunoglobulins supplement (MM), which were also administered for 7 days. We analyzed hepatic injury markers (AST, ALT) and creatinine, and inflammatory markers, IL-6, TNF-α, PGE2, iNOS, as well as total antioxidant capacity. The results obtained in the present study suggest that the modulation of the intestinal microbiota by administration of probiotics (Bacillus spores), alone or in combination with immunoglobulins and amino acids, represents an attractive therapy for the prevention of NSAID-induced enteropathy. MDPI 2022-10-07 /pmc/articles/PMC9599762/ /pubmed/36289770 http://dx.doi.org/10.3390/biomedicines10102508 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Neag, Maria-Adriana
Melincovici, Carmen-Stanca
Catinean, Adrian
Muntean, Dana-Maria
Pop, Raluca-Maria
Bocsan, Ioana-Corina
Mitre, Andrei-Otto
Cardos, Mihai-Bogdan
Inceu, Andreea-Ioana
Buzoianu, Anca-Dana
The Role of Probiotic Bacillus Spores and Amino Acids with Immunoglobulins on a Rat Enteropathy Model
title The Role of Probiotic Bacillus Spores and Amino Acids with Immunoglobulins on a Rat Enteropathy Model
title_full The Role of Probiotic Bacillus Spores and Amino Acids with Immunoglobulins on a Rat Enteropathy Model
title_fullStr The Role of Probiotic Bacillus Spores and Amino Acids with Immunoglobulins on a Rat Enteropathy Model
title_full_unstemmed The Role of Probiotic Bacillus Spores and Amino Acids with Immunoglobulins on a Rat Enteropathy Model
title_short The Role of Probiotic Bacillus Spores and Amino Acids with Immunoglobulins on a Rat Enteropathy Model
title_sort role of probiotic bacillus spores and amino acids with immunoglobulins on a rat enteropathy model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9599762/
https://www.ncbi.nlm.nih.gov/pubmed/36289770
http://dx.doi.org/10.3390/biomedicines10102508
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