Cargando…

Design and Optimization of A Magneto-Plasmonic Sandwich Biosensor for Integration within Microfluidic Devices

We designed a magneto-plasmonic biosensor for the immunodetection of antigens in minute sample volume. Both spherical gold nanoparticles (AuNP) and magnetic beads (MB) were conjugated to goat anti-rabbit IgG antibody (Ab) capable of recognizing a model target, rabbit IgG (rIgG). The AuNP bioconjugat...

Descripción completa

Detalles Bibliográficos
Autores principales: Soroush, Mona, Ait Mammar, Walid, Wilson, Axel, Ghourchian, Hedayatollah, Salmain, Michèle, Boujday, Souhir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9599771/
https://www.ncbi.nlm.nih.gov/pubmed/36290935
http://dx.doi.org/10.3390/bios12100799
_version_ 1784816675352215552
author Soroush, Mona
Ait Mammar, Walid
Wilson, Axel
Ghourchian, Hedayatollah
Salmain, Michèle
Boujday, Souhir
author_facet Soroush, Mona
Ait Mammar, Walid
Wilson, Axel
Ghourchian, Hedayatollah
Salmain, Michèle
Boujday, Souhir
author_sort Soroush, Mona
collection PubMed
description We designed a magneto-plasmonic biosensor for the immunodetection of antigens in minute sample volume. Both spherical gold nanoparticles (AuNP) and magnetic beads (MB) were conjugated to goat anti-rabbit IgG antibody (Ab) capable of recognizing a model target, rabbit IgG (rIgG). The AuNP bioconjugate was used as the optical detection probe while the MB one was used as the capture probe. Addition of the target analyte followed by detection probe resulted in the formation of a sandwich immunocomplex which was separated from the unbound AuNP-Ab conjugate by application of an external magnetic field. The readout was executed either in a direct or in indirect way by measuring the UV–Visible spectrum of each fraction in a specially designed microcell. Dose–response curves were established from the optical signal of the immunocomplex and unbound AuNP-Ab conjugate fractions. Finally, the assay was transposed to a microfluidic cell specially designed to enable easy separation of the immunocomplex and AuNP-Ab conjugate fractions and subsequent analysis of the latter fraction and achieve the quantification of the analyte in the ng/mL concentration range.
format Online
Article
Text
id pubmed-9599771
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-95997712022-10-27 Design and Optimization of A Magneto-Plasmonic Sandwich Biosensor for Integration within Microfluidic Devices Soroush, Mona Ait Mammar, Walid Wilson, Axel Ghourchian, Hedayatollah Salmain, Michèle Boujday, Souhir Biosensors (Basel) Article We designed a magneto-plasmonic biosensor for the immunodetection of antigens in minute sample volume. Both spherical gold nanoparticles (AuNP) and magnetic beads (MB) were conjugated to goat anti-rabbit IgG antibody (Ab) capable of recognizing a model target, rabbit IgG (rIgG). The AuNP bioconjugate was used as the optical detection probe while the MB one was used as the capture probe. Addition of the target analyte followed by detection probe resulted in the formation of a sandwich immunocomplex which was separated from the unbound AuNP-Ab conjugate by application of an external magnetic field. The readout was executed either in a direct or in indirect way by measuring the UV–Visible spectrum of each fraction in a specially designed microcell. Dose–response curves were established from the optical signal of the immunocomplex and unbound AuNP-Ab conjugate fractions. Finally, the assay was transposed to a microfluidic cell specially designed to enable easy separation of the immunocomplex and AuNP-Ab conjugate fractions and subsequent analysis of the latter fraction and achieve the quantification of the analyte in the ng/mL concentration range. MDPI 2022-09-27 /pmc/articles/PMC9599771/ /pubmed/36290935 http://dx.doi.org/10.3390/bios12100799 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Soroush, Mona
Ait Mammar, Walid
Wilson, Axel
Ghourchian, Hedayatollah
Salmain, Michèle
Boujday, Souhir
Design and Optimization of A Magneto-Plasmonic Sandwich Biosensor for Integration within Microfluidic Devices
title Design and Optimization of A Magneto-Plasmonic Sandwich Biosensor for Integration within Microfluidic Devices
title_full Design and Optimization of A Magneto-Plasmonic Sandwich Biosensor for Integration within Microfluidic Devices
title_fullStr Design and Optimization of A Magneto-Plasmonic Sandwich Biosensor for Integration within Microfluidic Devices
title_full_unstemmed Design and Optimization of A Magneto-Plasmonic Sandwich Biosensor for Integration within Microfluidic Devices
title_short Design and Optimization of A Magneto-Plasmonic Sandwich Biosensor for Integration within Microfluidic Devices
title_sort design and optimization of a magneto-plasmonic sandwich biosensor for integration within microfluidic devices
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9599771/
https://www.ncbi.nlm.nih.gov/pubmed/36290935
http://dx.doi.org/10.3390/bios12100799
work_keys_str_mv AT soroushmona designandoptimizationofamagnetoplasmonicsandwichbiosensorforintegrationwithinmicrofluidicdevices
AT aitmammarwalid designandoptimizationofamagnetoplasmonicsandwichbiosensorforintegrationwithinmicrofluidicdevices
AT wilsonaxel designandoptimizationofamagnetoplasmonicsandwichbiosensorforintegrationwithinmicrofluidicdevices
AT ghourchianhedayatollah designandoptimizationofamagnetoplasmonicsandwichbiosensorforintegrationwithinmicrofluidicdevices
AT salmainmichele designandoptimizationofamagnetoplasmonicsandwichbiosensorforintegrationwithinmicrofluidicdevices
AT boujdaysouhir designandoptimizationofamagnetoplasmonicsandwichbiosensorforintegrationwithinmicrofluidicdevices