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Effect of Cannabidiolic Acid, N-Trans-Caffeoyltyramine and Cannabisin B from Hemp Seeds on microRNA Expression in Human Neural Cells

Given the increasing interest in bioactive dietary components that can modulate gene expression enhancing human health, three metabolites isolated from hemp seeds—cannabidiolic acid, N-trans-caffeoyltyramine, and cannabisin B—were examined for their ability to change the expression levels of microRN...

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Autores principales: Di Palo, Armando, Siniscalchi, Chiara, Crescente, Giuseppina, De Leo, Ilenia, Fiorentino, Antonio, Pacifico, Severina, Russo, Aniello, Potenza, Nicoletta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9600072/
https://www.ncbi.nlm.nih.gov/pubmed/36286061
http://dx.doi.org/10.3390/cimb44100347
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author Di Palo, Armando
Siniscalchi, Chiara
Crescente, Giuseppina
De Leo, Ilenia
Fiorentino, Antonio
Pacifico, Severina
Russo, Aniello
Potenza, Nicoletta
author_facet Di Palo, Armando
Siniscalchi, Chiara
Crescente, Giuseppina
De Leo, Ilenia
Fiorentino, Antonio
Pacifico, Severina
Russo, Aniello
Potenza, Nicoletta
author_sort Di Palo, Armando
collection PubMed
description Given the increasing interest in bioactive dietary components that can modulate gene expression enhancing human health, three metabolites isolated from hemp seeds—cannabidiolic acid, N-trans-caffeoyltyramine, and cannabisin B—were examined for their ability to change the expression levels of microRNAs in human neural cells. To this end, cultured SH-SY5Y cells were treated with the three compounds and their microRNA content was characterized by next-generation small RNA sequencing. As a result, 31 microRNAs underwent major expression changes, being at least doubled or halved by the treatments. A computational analysis of the biological pathways affected by these microRNAs then showed that some are implicated in neural functions, such as axon guidance, hippocampal signaling, and neurotrophin signaling. Of these, miR-708-5p, miR-181a-5p, miR-190a-5p, miR-199a-5p, and miR-143-3p are known to be involved in Alzheimer’s disease and their expression changes are expected to ameliorate neural function. Overall, these results provide new insights into the mechanism of action of hemp seed metabolites and encourage further studies to gain a better understanding of their biological effects on the central nervous system.
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spelling pubmed-96000722022-10-27 Effect of Cannabidiolic Acid, N-Trans-Caffeoyltyramine and Cannabisin B from Hemp Seeds on microRNA Expression in Human Neural Cells Di Palo, Armando Siniscalchi, Chiara Crescente, Giuseppina De Leo, Ilenia Fiorentino, Antonio Pacifico, Severina Russo, Aniello Potenza, Nicoletta Curr Issues Mol Biol Article Given the increasing interest in bioactive dietary components that can modulate gene expression enhancing human health, three metabolites isolated from hemp seeds—cannabidiolic acid, N-trans-caffeoyltyramine, and cannabisin B—were examined for their ability to change the expression levels of microRNAs in human neural cells. To this end, cultured SH-SY5Y cells were treated with the three compounds and their microRNA content was characterized by next-generation small RNA sequencing. As a result, 31 microRNAs underwent major expression changes, being at least doubled or halved by the treatments. A computational analysis of the biological pathways affected by these microRNAs then showed that some are implicated in neural functions, such as axon guidance, hippocampal signaling, and neurotrophin signaling. Of these, miR-708-5p, miR-181a-5p, miR-190a-5p, miR-199a-5p, and miR-143-3p are known to be involved in Alzheimer’s disease and their expression changes are expected to ameliorate neural function. Overall, these results provide new insights into the mechanism of action of hemp seed metabolites and encourage further studies to gain a better understanding of their biological effects on the central nervous system. MDPI 2022-10-21 /pmc/articles/PMC9600072/ /pubmed/36286061 http://dx.doi.org/10.3390/cimb44100347 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Di Palo, Armando
Siniscalchi, Chiara
Crescente, Giuseppina
De Leo, Ilenia
Fiorentino, Antonio
Pacifico, Severina
Russo, Aniello
Potenza, Nicoletta
Effect of Cannabidiolic Acid, N-Trans-Caffeoyltyramine and Cannabisin B from Hemp Seeds on microRNA Expression in Human Neural Cells
title Effect of Cannabidiolic Acid, N-Trans-Caffeoyltyramine and Cannabisin B from Hemp Seeds on microRNA Expression in Human Neural Cells
title_full Effect of Cannabidiolic Acid, N-Trans-Caffeoyltyramine and Cannabisin B from Hemp Seeds on microRNA Expression in Human Neural Cells
title_fullStr Effect of Cannabidiolic Acid, N-Trans-Caffeoyltyramine and Cannabisin B from Hemp Seeds on microRNA Expression in Human Neural Cells
title_full_unstemmed Effect of Cannabidiolic Acid, N-Trans-Caffeoyltyramine and Cannabisin B from Hemp Seeds on microRNA Expression in Human Neural Cells
title_short Effect of Cannabidiolic Acid, N-Trans-Caffeoyltyramine and Cannabisin B from Hemp Seeds on microRNA Expression in Human Neural Cells
title_sort effect of cannabidiolic acid, n-trans-caffeoyltyramine and cannabisin b from hemp seeds on microrna expression in human neural cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9600072/
https://www.ncbi.nlm.nih.gov/pubmed/36286061
http://dx.doi.org/10.3390/cimb44100347
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